Journal: PLoS ONE

Article Title: Evaluating the field performance of multiple SARS-Cov-2 antigen rapid tests using nasopharyngeal swab samples

doi: 10.1371/journal.pone.0262399

Figure Lengend Snippet: List of tested SARS-CoV-2 Ag-RTDs and manufacturers.

Article Snippet: To detect viral nucleic acid RT-qPCR was conducted on the LightCycler 480 Instrument II platform using the PerkinElmer® SARS-CoV-2 Real-time RT-PCR Assay (PerkinElmer Inc., Waltham, Massachusetts, United States) to detect SARS-CoV-2 ORF1ab and N genes and human Rnase P gene as internal control (IC).

Techniques:

Journal: The Journal of Hospital Infection

Article Title: Efficiency and sensitivity optimization of a protocol to quantify indoor airborne SARS-CoV-2 levels

doi: 10.1016/j.jhin.2022.08.011

Figure Lengend Snippet: Reverse transcriptase (RT) efficiency and sensitivity comparison in detection of SARS-CoV-2 genome target by RT–droplet digital polymerase chain reaction. An RNA plasmid solution containing the ORF1ab target was serially diluted and cDNA was synthesized using two RTs. The number of measured ORF1ab target copies was plotted against the expected copies to obtain the slope, from which efficiency was calculated. RTs used were TranscriptMe (green) and SuperScript II (purple). (A) 10 2 –10 5 plasmid copy number range; (B) 1–10 3 plasmid copy number range.

Article Snippet: An RNA plasmid containing the ORF1ab target (TibMolbiol) was used to compare RT efficiencies, that were experimentally calculated by using a known number of plasmid copies.

Techniques: Reverse Transcription, Comparison, Digital PCR, Plasmid Preparation, Synthesized

Journal: The Journal of Hospital Infection

Article Title: Efficiency and sensitivity optimization of a protocol to quantify indoor airborne SARS-CoV-2 levels

doi: 10.1016/j.jhin.2022.08.011

Figure Lengend Snippet: Efficiency comparison between two reverse transcriptases (RT) in airborne SARS-CoV-2 RNA assessment by RT–droplet digital polymerase chain reaction. RNA isolated from air samples collected from COVID-19 patient rooms was reverse-transcribed using TranscriptMe (green) and SuperScript II (purple). (A) Percentage of samples in which SARS-CoV-2 was detected by analysing the ORF1ab and N1 targets (black circle portion). (B) Mean and standard error of target copy number per reaction. N = 30 per group.

Article Snippet: An RNA plasmid containing the ORF1ab target (TibMolbiol) was used to compare RT efficiencies, that were experimentally calculated by using a known number of plasmid copies.

Techniques: Comparison, Digital PCR, Isolation, Reverse Transcription

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Companies and laboratories that have received EUA for RT-PCR–based tests

Article Snippet: March 26, 2020 , BGI Genomics Co. Ltd , ORF1ab , β-Actin.

Techniques: Control, Luminex

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Examples of companies offering tests that are packaged in cartridges and POC tests

Article Snippet: March 26, 2020 , BGI Genomics Co. Ltd , ORF1ab , β-Actin.

Techniques:

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: EUAs for RT-PCR tests

Article Snippet: March 26, 2020 , BGI Genomics Co. Ltd , ORF1ab , β-Actin.

Techniques: Control, Diagnostic Assay

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: SARS-CoV-2 genome and RT-PCR primer/amplicon sites: Schematic of SARS-CoV-2 genome with localization of various published RT-PCR amplicons in ORF1ab/b, S, E, and N genes. Primer/amplicon sequences aligned with 2 highly similar viral consensus sequences (EPI_ISL_412026 [BetaCoV/Hefei/2/2020] and MT106052.1 [2019-nCoV/USA-CA7/2020]) using NCBI BLAST program. E , Envelope; ORF , Open Reading Frame; RdRp , RNA-dependent RNA polymerase; UTR , untranslated region. Primer sequences from referenced publications. , , US CDC primers (N1, N2, N3) as reported in Udugama et al.

Article Snippet: March 26, 2020 , BGI Genomics Co. Ltd , ORF1ab , β-Actin.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Companies and laboratories that have received EUA for RT-PCR–based tests

Article Snippet: April 18, 2020 , Osang Healthcare , ORF1ab , RNase P.

Techniques: Luminex

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Examples of companies offering tests that are packaged in cartridges and POC tests

Article Snippet: April 18, 2020 , Osang Healthcare , ORF1ab , RNase P.

Techniques:

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: EUAs for RT-PCR tests

Article Snippet: April 18, 2020 , Osang Healthcare , ORF1ab , RNase P.

Techniques: Diagnostic Assay

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: SARS-CoV-2 genome and RT-PCR primer/amplicon sites: Schematic of SARS-CoV-2 genome with localization of various published RT-PCR amplicons in ORF1ab/b, S, E, and N genes. Primer/amplicon sequences aligned with 2 highly similar viral consensus sequences (EPI_ISL_412026 [BetaCoV/Hefei/2/2020] and MT106052.1 [2019-nCoV/USA-CA7/2020]) using NCBI BLAST program. E , Envelope; ORF , Open Reading Frame; RdRp , RNA-dependent RNA polymerase; UTR , untranslated region. Primer sequences from referenced publications. , , US CDC primers (N1, N2, N3) as reported in Udugama et al.

Article Snippet: April 18, 2020 , Osang Healthcare , ORF1ab , RNase P.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Companies and laboratories that have received EUA for RT-PCR–based tests

Article Snippet: March 20, 2020 , Primerdesign Ltd , ORF1ab , None.

Techniques: Control, Luminex

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: Examples of companies offering tests that are packaged in cartridges and POC tests

Article Snippet: March 20, 2020 , Primerdesign Ltd , ORF1ab , None.

Techniques:

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: EUAs for RT-PCR tests

Article Snippet: March 20, 2020 , Primerdesign Ltd , ORF1ab , None.

Techniques: Control, Diagnostic Assay

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Clinical testing for COVID-19

doi: 10.1016/j.jaci.2020.05.012

Figure Lengend Snippet: SARS-CoV-2 genome and RT-PCR primer/amplicon sites: Schematic of SARS-CoV-2 genome with localization of various published RT-PCR amplicons in ORF1ab/b, S, E, and N genes. Primer/amplicon sequences aligned with 2 highly similar viral consensus sequences (EPI_ISL_412026 [BetaCoV/Hefei/2/2020] and MT106052.1 [2019-nCoV/USA-CA7/2020]) using NCBI BLAST program. E , Envelope; ORF , Open Reading Frame; RdRp , RNA-dependent RNA polymerase; UTR , untranslated region. Primer sequences from referenced publications. , , US CDC primers (N1, N2, N3) as reported in Udugama et al.

Article Snippet: March 20, 2020 , Primerdesign Ltd , ORF1ab , None.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification