Journal: Advanced Science

Article Title: WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma

doi: 10.1002/advs.202506529

Figure Lengend Snippet: BMP7 is a target of H4K8 lactylation in ESCC cells. A) The binding density of H4K8la was visualized by deepTools: the heatmap illustrates the CUT&Tag tag counts on the various H4K8la enrichment peaks in shNC and shPDIA3P1 cells. B) Distribution of H4K8la sites relative to translation start site (TSS). C Genome‐wide distribution of downregulated H4K8la‐binding peaks in shPDIA3P cells. D) KEGG analysis of the decreased H4K8la binding peaks at candidate target genes. E) Transcriptome sequencing was performed in control and shPDIA3P1 cells. F) Venn Diagram of CUT&Tag, RNA‐seq, GEO, and pubmed database to identify the potential downstream targets of H4K8la. G) Integrative Genomics Viewer tracks of CUT&Tag showing enriched H4K8la in the promotors of BMP7. The red rectangles indicate the peak regions of H4K8la on target‐gene promoters. H‐K) BMP7 mRNA and protein levels were measured in PDIA3P1‐KD cells (H, J) or PDIA3P1‐OE cells (I, K). L) Western blotting analysis of BMP7 expression in PDIA3P1‐KD cells cultured in lactate for 24 h. M) Western blot analysis of BMP7 expression in PDIA3P1‐OE cells cultured in 2‐DG or oxamate for 24 h. N, O) Using antibodies against H4K8la, CUT&Tag‐qPCR analysis for binding status at the BMP7 promotor of PDIA3P1‐KD cells (N) or PDIA3P1‐OE cells (O). These data represent the mean ± S.D. of triplicates. ** P < 0.01; *** P < 0.001.

Article Snippet: Immunohistochemistry (IHC) staining was performed with an antibody specific for BMP7 (12221‐1‐AP, Proteintech) and Ki‐67 (HA721115, HUABIO).

Techniques: Binding Assay, Genome Wide, Sequencing, Control, RNA Sequencing, Western Blot, Expressing, Cell Culture

Journal: Advanced Science

Article Title: WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma

doi: 10.1002/advs.202506529

Figure Lengend Snippet: PDIA3P1 promotes tumorigenesis of esophageal squamous cell carcinoma through BMP7 both in vitro and in vivo. A) Western blot detection of BMP7 expression to reflect the effect of transfection of BMP7 by lentivirus. B‐G) Using lentivirus to construct BMP7 stable overexpression cell lines in TE‐1 and Eca‐109 cells with stably PDIA3P1‐KD. B) Proliferation of cells was analyzed using CCK8 assay. C) Tumor growth of TE‐1 and Eca‐109 cells was evaluated by colony formation assay. D) The proliferative abilities of ESCC cells were investigated using EdU assays. EdU scale bar: 100 µm. E) The flow cytometry showing apoptosis of cells by Annexin V‐APC and 7‐AAD staining. F) The migration and invasion ability was assessed by transwell assays. Transwell scale bar: 10 µm. G) Western blot shows expression levels of E‐Cadherin, N‐Cadherin, Vimentin, Snail, and BMP7. H‐K) Eca‐109 cells transfected with control, shPDIA3P1 or shPDIA3P1 and BMP7 were injected subcutaneously to establish a tumorigenesis model in nude mice ( n = 6). H) Photograph and comparison of tumor sizes in the indicated groups. I) The parameters of subcutaneous tumors were measured and recorded every 5 days, and calculated the volume of the tumor according to the formula below: tumor volume = 0.5 × length × width × width (mm3). J) Tumor weights in the indicated groups. K) Ki‐67 and BMP7 expression of representative IHC of nude mice tumor tissues. Scale bar, 20 µm. IF staining of TUNEL in subcutaneous tumor tissue sections. Scale bar: 50 µm. These data represent the mean ± S.D. of triplicates. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Immunohistochemistry (IHC) staining was performed with an antibody specific for BMP7 (12221‐1‐AP, Proteintech) and Ki‐67 (HA721115, HUABIO).

Techniques: In Vitro, In Vivo, Western Blot, Expressing, Transfection, Construct, Over Expression, Stable Transfection, CCK-8 Assay, Colony Assay, Flow Cytometry, Staining, Migration, Control, Injection, Comparison, TUNEL Assay

Journal: International Journal of Molecular Sciences

Article Title: Effect of Inducible BMP-7 Expression on the Osteogenic Differentiation of Human Dental Pulp Stem Cells

doi: 10.3390/ijms22126182

Figure Lengend Snippet: ( A ) Representative images of the DPSC-BMP-7 cell line incubated in the presence (DOX) or absence (−DOX) of doxycycline. ( B ) Western blot analysis of DPSC-BMP7 cell lysates.

Article Snippet: Human BMP7 ELISA Kit (Proteintech Group, Rosemont, IL, USA) was used to assess the level of BMP-7 in the cell supernatants.

Techniques: Incubation, Western Blot

Journal: Nephro-urology monthly

Article Title: Changes in the Expression of Bone Morphogenetic Protein 7 and Tamm– Horsfall Protein in the Early Stages of Diabetic Nephropathy

doi: 10.5812/numonthly.2124

Figure Lengend Snippet: The Western Blot Analysis of the Protein Expression of BMP7 (a), Podocin (b) and THP (c). The Expression of β-actin Was Used as a Loading Control for the Normalization of the Expression of Three Interested Genes.

Article Snippet: The primary antibodies were prepared in TBST and incubated at 30°C for 2 h. The primary antibodies used to detect BMP7 and podocin (Wuhan Boster Biotechnology Co. Ltd.) were used at a 1:500 dilution and a 1:300 dilution, respectively.

Techniques: Western Blot, Expressing, Control

Journal: Nephro-urology monthly

Article Title: Changes in the Expression of Bone Morphogenetic Protein 7 and Tamm– Horsfall Protein in the Early Stages of Diabetic Nephropathy

doi: 10.5812/numonthly.2124

Figure Lengend Snippet: Quantification of the Expression of BMP7, Podocin, and THP in the Early Stage of Diabetic Nephropathy

Article Snippet: The primary antibodies were prepared in TBST and incubated at 30°C for 2 h. The primary antibodies used to detect BMP7 and podocin (Wuhan Boster Biotechnology Co. Ltd.) were used at a 1:500 dilution and a 1:300 dilution, respectively.

Techniques: Expressing