Journal: PloS one

Article Title: Transplantation of specific human astrocytes promotes functional recovery after spinal cord injury.

doi: 10.1371/journal.pone.0017328

Figure Lengend Snippet: Figure 1. Generation of two distinct types of astrocytes from human glial precursors after treatment with BMP4 or CNTF. Both BMP and CNTF promote the differentiation of hGPCs into GFAP-positive astrocytes expressing S100b but with otherwise distinct morphological and antigenic phenotypes. hGDAsBMP express lower levels of GFAP and exhibit a more compact morphology. hGDAsCNTF have a more elongated morphology and expressed high levels of GFAP. hGDAsCNTF also expressed high levels of neurite-outgrowth inhibitory chondroitin sulfate proteoglycans, phosphacan and CSPG4, as well as the transcription factor Olig2 - all of which have been found to be upregulated in glial scar

Article Snippet: Western blot analysis of chondroitin sulfate proteoglycans and Olig2 was performed as previously described [57], using anti-phosphacan monoclonal (1:1000, 3F8, Developmental Studies Hybridoma Bank), anti-CSPG4/NG2 monoclonal (1:2000, Chemicon), anti-Olig2 polyclonal (1:4000, Chemicon) and anti-b-tubulin monoclonal antibody (1:1000, Santa Cruz).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Mesenchymal Transition and PDGFRA Amplification/Mutation Are Key Distinct Oncogenic Events in Pediatric Diffuse Intrinsic Pontine Gliomas

doi: 10.1371/journal.pone.0030313

Figure Lengend Snippet: Panel A: Radial plot showing the expression of oligodendroglial markers in the two groups of DIPG, in log2 ratios related each other. The red circle represent the median expression level of the whole population of DIPG. Group 1 expresses higher levels of oligodendroglial markers than group 2 DIPG. Panel B: Morphological oligodendroglial differenciation in group 1 tumors (HES staining, ×40). Panel C: Morphological astrocytic differenciation in group 2 tumors (HES staining ×40). Panel D: Olig2 immunohistochemistry in a group 1 DIPG showing that probably not all cells in the biopsy are tumoral (×40). Panel E: Dual immunohistochemistry for Olig2 and GFAP showing that tumor cells in mitosis are GFAP negative but Olig2 positive (×100). Panel F: Overall survival of 55 DIPG according to the presence (red) or absence (blue) of oligodendroglial differenciation. Median OS was shorter in patients with oligodendroglial type of tumors (7.73 vs 12.37, p = 0.045, log rank test).

Article Snippet: Sections were then incubated with various commercial monoclonal primary antibodies against Olig2 (AF 2418, 1/150, R/D system, CA, USA), P53 (DO-1, 1/1, Ventana) and MIB-1 (1/100; Dako, Glostrup, Denmark).

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Inflammation

Article Title: MLKL Modulates Necroptosis and Neuroinflammation in a Mouse Model of MS

doi: 10.1007/s10753-025-02323-3

Figure Lengend Snippet: NSA inhibited microglia and astrocyte activation and activated oligodendrocytes in EAE mice. A - C Immunofluorescence staining was used to detect the protein expression of anti-Olig2, anti-Iba-1, and anti-GFAP in the lumbar spinal cords of the EAE and EAE + NSA groups. (Each Group n = 3. The data are expressed as the mean ± SEM. * P < 0.05, *** p < 0.001). D The protein expression levels of Olig2, Iba-1, and GFAP in the lumbar spinal lysate were detected by immunoblotting ( n = 6 per group). The data are expressed as the mean ± SEM. ** P < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: The proteins from the gel were transferred to PVDF membranes, and the membrane was blocked in rapid blocking solution for 15 min. Then incubated respectively with MLKL (Cell Signaling Technology, D2I6 N, Monoclonal, Rabbit IgG, 1:1000), RIPK3 (Cell Signaling Technology, E7 A7 F, Monoclonal, Rabbit IgG, 1:1000), P-MLKL (Affinity Biosciences, AF7420, Polyclonal, Rabbit, 1:1000), MyD88 (Abcam, ab219413, Rabbit monoclonal, 1:1000), IL-1β (ELK Biotechnology, ES3931, Polyclonal, Rabbit, 1:1000), Olig2 (Cell Signaling Technology, 65915, Monoclonal, Rabbit IgG, 1:1000), Iba-1 (Cell Signaling Technology, E404 W, Monoclonal, Rabbit IgG, 1:1000), GFAP (Cell Signaling Technology, D1 F4Q, Monoclonal, Rabbit IgG, 1:1000), NF-κB P65 (Cell Signaling Technology, D14E12, Monoclonal, Rabbit IgG, 1:1000), NLRP3 (Proteintech Group, 68102–1-Ig, Monoclonal, Mouse/IgG2a, 1:1000), GSDMD (Abcam, AB209845 , Monoclonal, Rabbit IgG, 1:1000), and caspase-1–20 (Proteinteech, 22915–1-AP, Polyclonal, Rabbit, 1:1000) antibodies overnight at 4 °C.

Techniques: Activation Assay, Immunofluorescence, Staining, Expressing, Western Blot

Journal: STAR Protocols

Article Title: Utilizing mouse optic nerve crush to examine CNS remyelination

doi: 10.1016/j.xpro.2021.100796

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Olig2 (1:300) , Novus biologicals , NBP1-28667.

Techniques: Transduction, Virus, Recombinant, Sterility, Saline, Imaging, Positive Control, Software, Microscopy