Journal: Reproductive Toxicology (Elmsford, N.y.)

Article Title: Effects of spike protein and toxin-like peptides found in COVID-19 patients on human 3D neuronal/glial model undergoing differentiation: possible implications for SARS-CoV-2 impact on brain development

doi: 10.1016/j.reprotox.2022.04.011

Figure Lengend Snippet: Genes and probes ID used for qPCR analysis (all from Thermo-Fisher).

Article Snippet: Oligodendrocyte Transcription Factor 1 , OLIG1 , Hs00744293_s1.

Techniques:

Journal: iScience

Article Title: miRNA-194-3p represses NF-κB in gliomas to attenuate iPSC genes and proneural to mesenchymal transition

doi: 10.1016/j.isci.2023.108650

Figure Lengend Snippet:

Article Snippet: Taqman gene expression assay probes were used to measure the mRNA levels of 194-1 (Thermo Fisher: Cat# Hs04231530_s1), 194-2 (Thermo Fisher: Cat# Hs04331541_s1), ALDH1A3 (Thermo Fisher: Cat# Hs00167476_m1), CD44 (Thermo Fisher: Cat# Hs05662929_s1), LYN (Thermo Fisher: Cat# Hs01015818_g1), WT1 (Thermo Fisher: Cat# Hs01103751_m1), CD133 (Thermo Fisher: Cat# Hs01009259_m1), Nestin (Thermo Fisher: Cat# Hs04187831_g1), OLIG1 (Thermo Fisher: Cat# Hs00907227_s1), SOX2 (Thermo Fisher: Cat# Hs04234836_s1), klf4 (Thermo Fisher: Cat# Hs00358836_m1), OCT4 (Thermo Fisher: Cat# Hs00999632_g1), FUT4 (Thermo Fisher: Cat# Hs01106466_s1), NCAM1 (Thermo Fisher: Cat# Hs00941830_m1), Nanog (Thermo Fisher: Cat# Hs02387400_g1), TAB2 (Thermo Fisher: Cat# Hs00248373_m1), CMYC (Thermo Fisher: Cat# Hs00153408_m1), DOCK5 (Thermo Fisher: Cat# Hs07287975_m1), FAS (Thermo Fisher: Cat# Hs00236330_m1), HFE (Thermo Fisher: Cat# Hs00373474_m1), LGALS3 (Thermo Fisher: Cat# Hs07288627_m1), MAL (Thermo Fisher: Cat# Hs00707014_s1), RHOF (Thermo Fisher: Cat# Hs00368032_m1), SOWAHA (Thermo Fisher: Cat# Hs00703106_s1), C21orf91 (Thermo Fisher: Cat# Hs05040994_s1), TRIM23 (Thermo Fisher: Cat# Hs01106626_m1).

Techniques: Recombinant, Protease Inhibitor, Transfection, Isolation, cDNA Synthesis, MTT Assay, Extraction, Transcription Factor Assay, Expressing, Plasmid Preparation, Reporter Assay, Staining, Negative Control, shRNA, Software

Journal: Journal of Neuroscience

Article Title: Ascl1/Mash1 Promotes Brain Oligodendrogenesis during Myelination and Remyelination

doi: 10.1523/jneurosci.0805-13.2013

Figure Lengend Snippet: Figure 1. Neonatal subsets of Ascl1-expressing cells in SVZ and CC include neuronal or oligodendroglial progenitors and OPCs. A–E, Sections through the dorsal SVZ and CC of P2 wild-type neonatesshowingbyimmunofluorescencetheexpressionofAscl1proteintogetherwithOlig2,Sox9,Sox10,andNkx2.2inPDGFR cells(OPCs,arrows)orPDGFR cells(arrowheads).A,Ascl1 iscoexpressedwithOlig2inimmatureprogenitors(PDGFR cells)andOPCsbothinSVZandCC,whereasAscl1iscoexpressedwithOlig1inPDGFR OPCsandfewPDGFR cellsoftheCC(B). C,Ascl1iscoexpressedwithSox9inallPDGFR OPCsandmostAscl1 /PDGFR cells,30%ofSVZcellsbeingSox9 .Ascl1iscoexpressedwithSox10(D)andNkx2.2(E)inallPDGFR OPCs butnotinPDGFR cells.F,Pan-Dlxantibody(recognizingDlx1/2/5/6proteins)labelshalfofAscl1 /PDGFR cells(grayarrowheadscomparedwithwhitearrowheads)butnotOPCs(arrows). G,Histogramsrepresentingthepercentageofcells(DAPI )intheSVZandCCthatexpressAscl1togetherwithotherTFs,usingPDGFRtolabelOPCs.Ascl1 representsAscl1-only-expressingcells (light gray), Ascl1 TF are PDGFR /Ascl1 cells coexpressing the other TF (light blue), TF Ascl1 are TF-only-expressing cells (dark blue). H, Summary figure representing in circles the Ascl1 populations both in SVZ and CC. In the SVZ, 10% of Ascl1 ,PDGFR cells are Ascl1-only-expressing cells (light gray), whereas 90% are Olig2 /Sox9 , and these can be subdivided in two halves by their expression of Dlx/Gsx2 (gray) or not (light blue). These two last populations are also present in the forming CC, where half of Ascl1 cells are OPCs (expressing PDGFR, Olig2, Olig1, Sox9, Sox10, and Nkx2.2). Dlx and Gsx2 are not expressed in OPCs, and most likely Ascl1 /Olig2 /Sox9 /Gsx2 /Dlx cells are oligodendroglial progenitors. Dotted line represents the border between SVZ and CC. Scale bars, 20 m.

Article Snippet: Human brain tissues were immunostained as previously described (Nait-Oumesmar et al., 2007) with mouse monoclonal antibodies for human Ascl1 (Cosmo Bio, 1:500), CD68 (clone KP1, Dako, 1:100), rabbit antibodies for Olig2 (Millipore, 1:100), Olig1 (R&D Systems, 1:50), and goat antibody for Sox10 (R&D Systems, 1:50).

Techniques: Expressing

Journal: PLoS Medicine

Article Title: Aberrant DNA Methylation of OLIG1, a Novel Prognostic Factor in Non-Small Cell Lung Cancer

doi: 10.1371/journal.pmed.0040108

Figure Lengend Snippet: OLIG1 immunohistochemistry in H1299 cells and OLIG1 deletion analysis. (A) Real-time PCR data for OLIG1 , BAHD1, and DMRTA1 normalized to the expression of CAMKK2 . The error bars indicate the standard deviation of nine different measurements per gene. The value for normal lung was established by taking the average mRNA expression of three different samples so as to try to capture the potential variability in the normal expression of each of the genes. ***; significant at the 97.5% confidence level; N, normal lung; A, adenocarcinoma; S, SCC. (B) OLIG1, BAHD1, and DMRTA1 mRNA expression in A549 and H1299 cell lines treated with 1 μM 5-aza-dC for 72 h. The error bars indicate the standard deviation of nine individual measurements. (C) OLIG1 immunohistochemistry on untreated and 1 μM 5-aza-dC treated H1299 cells. Treatment times are indicated underneath each panel. Photographs were taken at 400× magnification. (D) OLIG1 DNA level for adenocarcinomas and SCCs. Each bar represents one sample. The error bars indicate the standard deviation of three independent measurements. Samples for which the level of OLIG1 DNA was significantly lower than that of its matching tumor-free lung DNA ( p < 0.050, one-tail Student's t-test) are indicated with a star.

Article Snippet: A mouse monoclonal anti-OLIG1 antibody (R&D Systems, http://www.rndsystems.com ) was used at 1:1,000 dilution for immunohistochemical detection.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation

Journal: PLoS Medicine

Article Title: Aberrant DNA Methylation of OLIG1, a Novel Prognostic Factor in Non-Small Cell Lung Cancer

doi: 10.1371/journal.pmed.0040108

Figure Lengend Snippet: (A) OLIG1 gene diagram and luciferase activity determined for four deletion constructs in A549 cells. The gene is represented by a gray box with an arrow indicating the transcription start site. The location of the AscI site is indicated. The E2F3a promoter was used as a positive control for luciferase activity. The error bars indicate the standard deviation of three independent triplicate transfections. The gene diagram and constructs are drawn up to scale. (B) Bisulfite DNA sequencing of OLIG1 in two adenocarcinomas, two SCCs, and four tumor-free lung samples derived from the same patients. The gene diagram on top indicates the relative location of the sequenced products in relation to the CpG sites within the CpG island (vertical lines), the transcription start site (horizontal arrow), and the exon (gray box). A total of eight to ten clones were sequenced per sample. Each line represents an individual clone and each circle represents a CpG dinucleotide. Solid circle, methylated cytosines; open circle, unmethylated cytosines; vertical arrow, AscI site.

Article Snippet: A mouse monoclonal anti-OLIG1 antibody (R&D Systems, http://www.rndsystems.com ) was used at 1:1,000 dilution for immunohistochemical detection.

Techniques: Luciferase, Activity Assay, Construct, Positive Control, Standard Deviation, Transfection, DNA Sequencing, Derivative Assay, Clone Assay, Methylation

Journal: PLoS Medicine

Article Title: Aberrant DNA Methylation of OLIG1, a Novel Prognostic Factor in Non-Small Cell Lung Cancer

doi: 10.1371/journal.pmed.0040108

Figure Lengend Snippet: OLIG1 immunohistochemistry on (A and E) tumor-free lung, (B) an OLIG1 negative adenocarcinoma, and (F) an OLIG1 negative SCC; (C) a low OLIG1 expressing adenocarcinoma and (G) a low OLIG1 expressing SCC are shown; (D) a high OLIG1 expressing adenocarcinoma and (H) a high OLIG1 expressing SCC are shown. All images were acquired at 400× magnification.

Article Snippet: A mouse monoclonal anti-OLIG1 antibody (R&D Systems, http://www.rndsystems.com ) was used at 1:1,000 dilution for immunohistochemical detection.

Techniques: Immunohistochemistry, Expressing