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Image Search Results
Journal: Journal for Immunotherapy of Cancer
Article Title: Responses of patients with cancer to mRNA vaccines depend on the time interval between vaccination and last treatment
doi: 10.1136/jitc-2023-007387
Figure Lengend Snippet: T cell response to BNT162b2 mRNA vaccination in patients with cancer. (A) Representative flow cytometry plots showing IFNg expression of CD8 + (upper row) and CD4 + (lower row) T cells in a healthy individual after stimulation with DMSO (negative control), spike pool 1 (S1), spike pool 2 (S2) and PMA and Ionomycin (positive control), respectively. Percentage of IFNg expressing CD8 + (B) and CD4 + (C) T cells in patients with cancer receiving checkpoint inhibition (Checkpoint, n=4), tyrosine kinase inhibitors (TKI, n=9), B cell depleting therapies (anti-CD20, n=5), antibody therapy (n=8), immunotherapy with concomitant chemotherapy (Cht-ICI, n=12), chemotherapy (chemo, n=24) and corticosteroids (cortisone, n=31) and healthy individuals (HD, n=9) after stimulation with S1 and S2, respectively. Each dot represents one donor and was calculated by background subtraction. Mann-Whitney U test was performed to calculate significance with *p<0.05, **p<0.01.
Article Snippet: Single cell suspensions were stained using in house labeled monoclonal anti-human antibodies:
Techniques: Flow Cytometry, Expressing, Negative Control, Positive Control, Inhibition, MANN-WHITNEY
Journal: Journal for Immunotherapy of Cancer
Article Title: Responses of patients with cancer to mRNA vaccines depend on the time interval between vaccination and last treatment
doi: 10.1136/jitc-2023-007387
Figure Lengend Snippet: Third BNT162b2 boost vaccination in patients with ongoing B cell depleting therapies. Blood and serum samples were obtained one to 5 months post third vaccination with BNT162b2. (A) Anti-SARS-CoV-2 spike-specific IgG titers (U/mL) compared between patients receiving anti-CD20 therapy (n=9) and healthy donors (HD; n=9). Percentage of IFNg producing CD8 + (B) and CD4 + (C) T cells in after stimulation with spike pool 1 (S1) or spike pool 2 (S2) compared between patients receiving anti-CD20 therapy (n=19) and (HD; n=9). Mann-Whitney U test was performed to calculate significance with *p<0.05, **p<0.01, ***p<0.001 and ns not significant.
Article Snippet: Single cell suspensions were stained using in house labeled monoclonal anti-human antibodies:
Techniques: MANN-WHITNEY
Journal: International Journal of Molecular Sciences
Article Title: Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation
doi: 10.3390/ijms25052801
Figure Lengend Snippet: Monoclonal antibodies used in flow cytometry.
Article Snippet: CD8 , APC-Cy7 ,
Techniques: Cytometry, Conjugation Assay