Journal: BMC Research Notes

Article Title: A survey of pathogens associated with Cyperus esculentus L (tiger nuts) tubers sold in a Ghanaian city

doi: 10.1186/1756-0500-7-343

Figure Lengend Snippet: Zone of inhibition recorded by bacteria isolated from tiger nuts bought from different locations. (a) Staphylococcus spp (b) Enterobacter cloacae (c) Klebsiella oxytoca (d) Proteus vulgaris (e) Enterobacter spp. The antibiotics used, and the value of diameter measurement that were considered sensitive are: AS- ampicillin ≥15, BA- cotrimoxazole ≥16, CH- chloramphenicol ≥18, CP- ciprofloxacin ≥21, CI-ceftizoxime ≥ 25, GM-gentamicin ≥ 15, TZP- piperacillin ≥21, AK-amikacin ≥ 17, LE-levofloxacin ≥ 17, OF-ofloxacin ≥ 16, TE-tetracycline ≥ 15, CF- cefotaxime ≥26. Numbers on each bar indicate the exact measurement of the inhibition zone in mm.

Article Snippet: The antibiotics tested included gentamicin, amikacin, tetracycline, cotrimoxazole, cefotaxime, ceftizoxime, ampicillin, piperacillin, chloramphenicol, ciprofloxacin, levofloxacin and ofloxacin (Oxoid Ltd., Basingstoke, UK).

Techniques: Inhibition, Bacteria, Isolation

Journal: BMC Research Notes

Article Title: A survey of pathogens associated with Cyperus esculentus L (tiger nuts) tubers sold in a Ghanaian city

doi: 10.1186/1756-0500-7-343

Figure Lengend Snippet: Antibiotic sensitivity pattern of bacteria isolated from tiger nuts

Article Snippet: The antibiotics tested included gentamicin, amikacin, tetracycline, cotrimoxazole, cefotaxime, ceftizoxime, ampicillin, piperacillin, chloramphenicol, ciprofloxacin, levofloxacin and ofloxacin (Oxoid Ltd., Basingstoke, UK).

Techniques: Bacteria, Isolation

Journal: Toxics

Article Title: The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish ( Danio rerio )

doi: 10.3390/toxics13020122

Figure Lengend Snippet: Developmental toxicity of levofloxacin exposure in zebrafish embryos. ( A ) Hatching rates of zebrafish embryos exposed to levofloxacin at 72 hpf; ( B ) survival rates of zebrafish embryos exposed to levofloxacin at 96 hpf; ( C ) survival rates of zebrafish larvae at different time points under different concentrations of levofloxacin. * p < 0.05; ** p < 0.01 vs. 0 mM.

Article Snippet: We obtained the following: levofloxacin (CAS: 100986-85-4, purity > 99%, MedChemexpress, Monmouth Junction, NJ, USA); TRIzol reagent (15596018, Thermo Fisher Scientific, Waltham, MA, USA); Reverse Transcription Kit (0521751, Suzhou, Nearshore Protein Science and Technology Co., Hangzhou, China) Magic SYBR Mixture (CW3008M, Kangwei Century, Beijing, China); embryo culture medium (Shanghai Feixi Biotechnology Co., Ltd., Shanghai, China); and 3-ethoxyphenylmethanesulfonate (CAS: 886-86-2, Aladdin, Shanghai, China).

Techniques:

Journal: Toxics

Article Title: The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish ( Danio rerio )

doi: 10.3390/toxics13020122

Figure Lengend Snippet: Morphological effects of levofloxacin exposure on zebrafish larvae at 96 hpf and 120 hpf. ( A ) Body length at 96 hpf; ( B ) yolk sac area at 96 hpf; ( C ) swim bladder area at 96 hpf; ( D ) body length at 120 hpf; ( E ) yolk sac area at 120 hpf; ( F ) swim bladder area at 120 hpf. * p < 0.05; ** p < 0.01; **** p < 0.0001 vs. 0 mM. ( G ) Comparison of body length between 96 hpf and 120 hpf; ( H ) comparison of yolk sac area between 96 hpf and 120 hpf; ( I ) comparison of swim bladder area between 96 hpf and 120 hpf. # p < 0.05; ## p < 0.01; ### p < 0.001; #### p < 0.0001 vs. same concentration at 96 hpf.

Article Snippet: We obtained the following: levofloxacin (CAS: 100986-85-4, purity > 99%, MedChemexpress, Monmouth Junction, NJ, USA); TRIzol reagent (15596018, Thermo Fisher Scientific, Waltham, MA, USA); Reverse Transcription Kit (0521751, Suzhou, Nearshore Protein Science and Technology Co., Hangzhou, China) Magic SYBR Mixture (CW3008M, Kangwei Century, Beijing, China); embryo culture medium (Shanghai Feixi Biotechnology Co., Ltd., Shanghai, China); and 3-ethoxyphenylmethanesulfonate (CAS: 886-86-2, Aladdin, Shanghai, China).

Techniques: Comparison, Concentration Assay

Journal: Toxics

Article Title: The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish ( Danio rerio )

doi: 10.3390/toxics13020122

Figure Lengend Snippet: Morphological effects of levofloxacin exposure on zebrafish larvae. The red arrow indicates the yolk sac, the blue arrow indicates the swim bladder, and the red dashed line highlights the pericardial area. SV-BA: Length of sinus venosus to bulbus arteriosus.

Article Snippet: We obtained the following: levofloxacin (CAS: 100986-85-4, purity > 99%, MedChemexpress, Monmouth Junction, NJ, USA); TRIzol reagent (15596018, Thermo Fisher Scientific, Waltham, MA, USA); Reverse Transcription Kit (0521751, Suzhou, Nearshore Protein Science and Technology Co., Hangzhou, China) Magic SYBR Mixture (CW3008M, Kangwei Century, Beijing, China); embryo culture medium (Shanghai Feixi Biotechnology Co., Ltd., Shanghai, China); and 3-ethoxyphenylmethanesulfonate (CAS: 886-86-2, Aladdin, Shanghai, China).

Techniques:

Journal: Toxics

Article Title: The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish ( Danio rerio )

doi: 10.3390/toxics13020122

Figure Lengend Snippet: Cardiotoxic effects of levofloxacin exposure on zebrafish larvae. ( A ) Pericardial area of zebrafish larvae; ( B ) SV-BA length of zebrafish larvae; ( C ) ratio of SV-BA to body length of zebrafish larvae; ( D ) heart rate (beats per minute) of zebrafish larvae after 72 h of levofloxacin exposure; ( E – H ) expression of cardiac development marker genes ( nppa , myh6 , myl7 , cacna1ab ); ( I – L ) expression levels of major transcription factors ( gata4 , nkx2.5 , tbx2b , tbx5b ). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. 0 mM.

Article Snippet: We obtained the following: levofloxacin (CAS: 100986-85-4, purity > 99%, MedChemexpress, Monmouth Junction, NJ, USA); TRIzol reagent (15596018, Thermo Fisher Scientific, Waltham, MA, USA); Reverse Transcription Kit (0521751, Suzhou, Nearshore Protein Science and Technology Co., Hangzhou, China) Magic SYBR Mixture (CW3008M, Kangwei Century, Beijing, China); embryo culture medium (Shanghai Feixi Biotechnology Co., Ltd., Shanghai, China); and 3-ethoxyphenylmethanesulfonate (CAS: 886-86-2, Aladdin, Shanghai, China).

Techniques: Expressing, Marker

Journal: Toxics

Article Title: The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish ( Danio rerio )

doi: 10.3390/toxics13020122

Figure Lengend Snippet: Effects of 96 h levofloxacin exposure on zebrafish larval swimming behavior. ( A ) Low-to-moderate speed movement distance of zebrafish larvae in each concentration group; ( B ) high-speed movement distance of zebrafish larvae in each concentration group; ( C ) total movement distance of zebrafish larvae in each concentration group; ( D ) swimming trajectories of zebrafish larvae in each concentration group. Green trajectories represent low-to-moderate speed movement (1–12 cm/s), and red trajectories represent high-speed movement (>12 cm/s). ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. 0 mM.

Article Snippet: We obtained the following: levofloxacin (CAS: 100986-85-4, purity > 99%, MedChemexpress, Monmouth Junction, NJ, USA); TRIzol reagent (15596018, Thermo Fisher Scientific, Waltham, MA, USA); Reverse Transcription Kit (0521751, Suzhou, Nearshore Protein Science and Technology Co., Hangzhou, China) Magic SYBR Mixture (CW3008M, Kangwei Century, Beijing, China); embryo culture medium (Shanghai Feixi Biotechnology Co., Ltd., Shanghai, China); and 3-ethoxyphenylmethanesulfonate (CAS: 886-86-2, Aladdin, Shanghai, China).

Techniques: Concentration Assay

Journal: Biopharmaceutics & drug disposition

Article Title: Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells.

doi: 10.1002/bdd.679

Figure Lengend Snippet: Figure 2. Na1 (A) and pH (B) dependence of cellular uptake of levofloxacin in LS180 cells. Cells were incubated with [14C]levofloxacin for 15min at 371C (open circles) or 41C (closed circles) in the presence of 20mM unlabeled levofloxacin. Each point represents the mean7SE for 4–14 measurements. po0.05: significantly different from pH 7.4 at 371C

Article Snippet: Levofloxacin hydrochloride was purchased from LKT Laboratories (St Paul, MN, USA).

Techniques: Incubation

Journal: Biopharmaceutics & drug disposition

Article Title: Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells.

doi: 10.1002/bdd.679

Figure Lengend Snippet: Figure 3. Inhibitory effect of various compounds on cellular uptake of levofloxacin in LS cells. Cells were incubated with [14C]levofloxacin for 15 min in the presence of 100 mM unlabeled levofloxacin and various compounds. Closed column represents the mean7SE for 15 measurements, and open columns represent the mean7SE for 6–8 measurements. po0.05: significantly different from the control

Article Snippet: Levofloxacin hydrochloride was purchased from LKT Laboratories (St Paul, MN, USA).

Techniques: Incubation, Control

Journal: Biopharmaceutics & drug disposition

Article Title: Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells.

doi: 10.1002/bdd.679

Figure Lengend Snippet: Figure 4. Inhibitory effect of various compounds, including OATP inhibitors, on cellular uptake of levofloxacin in LS180 cells. Cells were incubated with [14C]levofloxacin for 15 min in the presence of 100 mM unlabeled levofloxacin and various compounds. Closed column represents the mean7SE for 15 measurements, and open columns represent the mean7SE for 6–8 measurements. po0.05: significantly different from the control

Article Snippet: Levofloxacin hydrochloride was purchased from LKT Laboratories (St Paul, MN, USA).

Techniques: Incubation, Control

Journal: Biopharmaceutics & drug disposition

Article Title: Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells.

doi: 10.1002/bdd.679

Figure Lengend Snippet: Figure 7. Inhibitory effect of choline chloride on cellular uptake of L-carnitine and levofloxacin in LS180 cells. (A) Cells were incubated with L-[3H]carnitine for 15min in the presence of 10mM unlabeled L-carnitine and choline chloride. (B) Cells were incubated with [14C]levofloxacin for 15 min in the presence of 10mM unlabeled levofloxacin and choline chloride. Each column represents the mean7SE for six measurements. po0.05: significantly different from the control

Article Snippet: Levofloxacin hydrochloride was purchased from LKT Laboratories (St Paul, MN, USA).

Techniques: Incubation, Control

Journal: Biopharmaceutics & drug disposition

Article Title: Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells.

doi: 10.1002/bdd.679

Figure Lengend Snippet: Figure 8. Inhibitory effect of ACE inhibitors on cellular uptake of levofloxacin in LS180 cells. Cells were incubated with [14C]levofloxacin for 15 min in the presence of 100 mM unlabeled levofloxacin and 1 mM ACE inhibitors. Closed column represents the mean7SE for 10 measurements, and open columns represent the mean7SE for 5–7 measurements. po0.05: significantly different from the control

Article Snippet: Levofloxacin hydrochloride was purchased from LKT Laboratories (St Paul, MN, USA).

Techniques: Incubation, Control