Journal: BMC Neuroscience

Article Title: Development of a central nervous system axonal myelination assay for high throughput screening

doi: 10.1186/s12868-016-0250-2

Figure Lengend Snippet: Flow scheme for the cortical cell myelination assay. a Dissociated cells from the cortex containing neurons and glial progenitor cells were cultured from E18 rat embryos onto poly- d -lysine/laminin coated 96-well plates. b On DIV4, when axonal projections ( red ) are apparent in the neuronal population, the growing co-culture was changed to MyM media to induce OL differentiation and initiate myelination. The following day test compounds were added and cultures were left undisturbed for an additional 8 days. c Cells were fixed and immunostained for MBP, Olig2 and DAPI on DIV13. Images were acquired using automated microscopy and scored phenotypically for myelination as described in the “ ”

Article Snippet: The dissection of E18 rat (Charles River, Wilmington, MA, USA) cortex is similar to that described previously [ – ] with some modifications.

Techniques: Cell Culture, Co-Culture Assay, Microscopy