Journal: Biomedicines

Article Title: Special delEVery: Extracellular Vesicles as Promising Delivery Platform to the Brain

doi: 10.3390/biomedicines9111734

Figure Lengend Snippet: Overview unmodified EVs applied for brain delivery. This table summarizes the experimental set ups and read-outs of the different studied EV types in brain delivery or brain disease EV treatment studies. Furthermore, information about the EV characterization based on the Minimal Information for Studies of EVs (MISEV) guidelines is included [ 3 ]. The “+” symbol in the protein marker column indicates the assessment of typical EV markers whereas the “-” symbol indicates the assessment of non-EV markers. Abbreviations: EV: extracellular vesicle; MoA: mode of action; ND: not determined; IV: intravenous; IP: intraperitoneal; IN: intranasal; UC: ultracentrifugation; SEC: size exclusion chromatography; TEM: transmission electron microscopy; AFM: atomic force microscopy; cpm: counts per minute; NTA: nanoparticle tracking analysis; DLS: dynamic light scattering; TRPS: tunable resistive pulse sensing; MRI: magnetic resonance imaging; CNP: cellular nanoporation; IVIS: in vivo imaging system; SRM: label-free selective reaction monitoring; Dil: 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine; DiR: 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide; CM: conditioned medium; PEG: Polyethylene glycol; PEI: polyethyleneimine; LDLR: Low density lipoprotein receptor; GBM: glioblastoma; DC: dendritic cell; NSC: neuronal stem cells; MSC: mesenchymal stem cells; ESC: embryonic stem cells; LPS: lipopolysaccharide; WGA: wheatgerm agglutinin; 6-OHDA: 6-hydroxydopamine; α-syn: alpha-synuclein; PFF: preformed fibril; PD: Parkinson’s disease; MCAO: middle cerebral artery occlusion; OGD: Oxygen-glucose deprivation; SCI: Spinal cord injury; HNSCC: head and neck squamous cell carcinoma; HPV: Human papilloma virus.

Article Snippet: EL-4 lymphoblast cell line (mouse) , 10,000 g centrifugation pellet + sucrose gradient centrifugation (post-loading) , ND , ND , ND , In vivo: 3 therapeutic models: -LPS brain inflammation -MOG-peptide induced EAE MS mouse model and -GL26-Luciferase brain tumor-bearing model mouse , 10 μg EV protein/mouse , IN , ND , IRDye800 label DiR label PKH26 label (incubation) Curcumin or JSI-124 (incubation) , DiR labelled EV detection in brain (Odyssey laser scanning imager, Carestream Molecular Imaging system) Brain sections PKH26 labelled EVs (confocal imaging) Curcumin load detection in brain + functional effects , Zhuang et al. [ ] .

Techniques: Marker, Size-exclusion Chromatography, Transmission Assay, Electron Microscopy, Microscopy, Tunable Resistive Pulse Sensing, Magnetic Resonance Imaging, In Vivo Imaging, Virus, Isolation, In Vivo, In Vitro, Injection, Incubation, Imaging, Expressing, Transfection, Functional Assay, Ligation, Variant Assay, Luciferase, Centrifugation, Gradient Centrifugation, Binding Assay, Single Photon Emission Computed Tomography, Labeling, Sonication, Derivative Assay, Produced, Mouse Assay, Confocal Microscopy, Construct, Transduction