Journal: Journal of Cellular and Molecular Medicine

Article Title: Bazi Bushen attenuates osteoporosis in SAMP6 mice by regulating PI3K ‐ AKT and apoptosis pathways

doi: 10.1111/jcmm.70161

Figure Lengend Snippet: Bazi Bushen improved the expression of stemness biomarkers and aging‐related proteins in BMSCs. (A) Western blot analysis of NANOG, SOX2, OCT4 and GAPDH in BMSCs. (B) Quantification of band intensities of NANOG relative to GAPDH in BMSCs. (C) Quantification of band intensities of SOX2 relative to GAPDH in BMSCs. (D) Quantification of band intensities of OCT4 relative to GAPDH in BMSCs. (E) Western blot analysis of p16, p21 and GAPDH in BMSCs. (F) Quantification of band intensities of p16 relative to GAPDH in BMSCs. (G) Quantification of band intensities of p21 relative to GAPDH in BMSCs. ## p < 0.01, # p < 0.05 versus the SAMR1 group; ** p < 0.01, * p < 0.05 versus the SAMP6 group ( n = 3–4).

Article Snippet: OCT4 (A7920), SOX2 (A0561), and NANOG (A3232) were provided by ABclonal Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Oncotarget

Article Title: NFATc3 plays an oncogenic role in oral/oropharyngeal squamous cell carcinomas by promoting cancer stemness via expression of OCT4

doi: 10.18632/oncotarget.26774

Figure Lengend Snippet: ( A ) Effect of NFATc3 on pluripotent transcription factors (NANOG, OCT4, KLF4, LIN28, and SOX2) expression was determined by qPCR. Their levels in NOKSI/NFATc3 were plotted as fold change against those in NOKSI/EV. * P < 0.001. ( B ) Effect of NFATc3 on OCT4 promoter activity was determined by luciferase promoter assay. Cells were transfected with pGL3-Basic (promoter-less) or pGL3 vectors containing the 1.5-kb upstream (-1545∼ -24) of Oct4. * P < 0.001. ( C ) Sequence analysis reveals a consensus NFAT binding site (5′-GGAAA-3′) at -1088 ∼ -1084 indicated by star (upper diagram). OSCC cells were lysed and performed a ChIP assay. The fragment (-1191∼ -1061) containing the NFAT binding site was enriched with NFATc3, and the fragment (-2930∼ -2783) was amplified as a control. * P < 0.01. ( D ) Correlation analysis of NFATc3 and OCT4 mRNA was determined based on their expression levels in 18 human SCC cell lines by qPCR.

Article Snippet: The mammalian pCMV6-Entry Vector encoding OCT4 was purchased from OriGene (RC211998).

Techniques: Expressing, Activity Assay, Luciferase, Promoter Assay, Transfection, Sequencing, Binding Assay, Amplification

Journal: Oncotarget

Article Title: NFATc3 plays an oncogenic role in oral/oropharyngeal squamous cell carcinomas by promoting cancer stemness via expression of OCT4

doi: 10.18632/oncotarget.26774

Figure Lengend Snippet: ( A ) The effect of OCT4 knockdown on self-renewal capacity of NOKSI/NFATc3 was determined by tumor sphere formation assay. OCT4 was knocked down in NOKSI/NFATc3 using siRNA against OCT4 (Oct4i). The cells transfected with control siRNA (CTLi) were included for comparison. * P < 0.05. ( B ) The effect of OCT4 knockdown on migration ability in NOKSI/NFATc3 was determined by transwell migration assay. ** P < 0.01. ( C ) The effect of OCT4 knockdown on self-renewal capacity of SCC4 was determined by tumor sphere formation assay. ( D ) The effect of OCT4 knockdown on self-renewal capacity of SCC4 was determined by transwell migration assay. ( E ) OCT4 expression was forced in non-tumorigenic immortalized oral epithelial cells, NOKSI, by vector expressing recombinant Myc-DDK-tagged OCT4, and its ectopic expression was confirmed by Western blot analysis using anti-DDK antibody. ( F ) Effect of ectopic OCT4 expression on self-renewal capacity of NOKSI was determined by tumor sphere formation assay. Representative images of tumor spheres formed by NOKSI/EV and NOKSI/Oct4 are shown on the right. ( G ) Effect of ectopic OCT4 expression on migration ability of NOKSI was determined by transwell migration assay. ( H ) Effect of ectopic OCT4 expression on the expression of NFAT isoforms (NFATc1-c4) in NOKSI was determined by qPCR. Their levels in NOKSI/Oct4 were plotted as fold induction against those in NOKSI/EV.

Article Snippet: The mammalian pCMV6-Entry Vector encoding OCT4 was purchased from OriGene (RC211998).

Techniques: Tube Formation Assay, Transfection, Migration, Transwell Migration Assay, Expressing, Plasmid Preparation, Recombinant, Western Blot

Journal: BMC Ophthalmology

Article Title: Comparison of two different optical coherence tomography angiography devices in detecting healthy versus glaucomatous eyes – an observational cross-sectional study

doi: 10.1186/s12886-020-01701-9

Figure Lengend Snippet: Representative images from AngioVue and SweptSource optical coherence tomography angiography (OCTA) devices. Optic disc scans using AngioVue are divided into ( a ) vitreous, ( b ) radial peripapillary capillaries, ( c ) nerve head, and ( d ) disc choroid layers. Macular scans using AngioVue are divided into ( e ) superficial retina, ( f ) deep retina, ( g ) outer retina, and ( h ) choroid layers. Example of quadrant division of ( i ) optic disc scan and ( j ) macular scan using AngioVue. Optic disc scans (raw images shown) using SweptSource are divided into ( k ) superficial retina, ( l ) deep retina, and ( m ) choroid layers. Similarly, macular scans (raw images shown) using SweptSource are divided into ( n ) superficial retina, ( o ) deep retina, and ( p ) choroid layers

Article Snippet: The Swept Source OCTA (SS-OCTA) (DRI-OCT Atlantis OCT, Topcon Corporation, Japan) uses a 1050 nm wavelength system and has an A-scan rate of 100,000 scans per second.

Techniques: Tomography