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Image Search Results
Journal: bioRxiv
Article Title: Multiplexed and Inducible Gene Modulation in Human Pluripotent Stem Cells by CRISPR Interference and Activation
doi: 10.1101/603951
Figure Lengend Snippet: A. Relative locations and sequences of TCF4 gRNAs. Numbers correspond to genetic coordinates in hg38 human genome assembly. B. Confirmation of presence of 3x gRNA insert in multi-gRNA PB vectors by digestion with BamHI restriction enzyme. C. Representative immunostaining for pluripotency markers SSEA4, TRA-1-60, and OCT4 in dCas9-KRAB-PB and dCas9-VPR-PB cells. D. Representative immunostaining for AFP (endoderm), SMA (mesoderm) and β-III Tubulin (ectoderm) following embryoid body formation from dCas9-KRAB-PB and dCas9-VPR-PB cells. Cells are counterstained with DAPI.
Article Snippet: The following primary antibodies were used:
Techniques: Immunostaining