Journal: Cell reports

Article Title: Functional Oocytes Derived from Granulosa Cells.

doi: 10.1016/j.celrep.2019.11.080

Figure Lengend Snippet: Figure 2. gPSCs Acquire High Germline Competence (A) Morphology and expression of pluripotency markers of gPSCs and female ESCs. Scale bar, 30 mm. (B) Low level of methylation at Oct4 and Nanog promoter regions in gPSCs. White dots indicate unmethylated loci, and dark spots indicate methylated loci. (C) Germline competency of gPSCs and female ESCs by injection into 4- to 8-cell embryos and genotyping by D12Mit136 microsatellite assay (right panel). Albino BALB/c mice served as embryo donors, and pseudo-pregnant albino Kunming mice served as surrogate mothers. (D) Summary of chimeras and germline transmission (GT) pups by injection into 4- to 8-cell embryos of gPSCs and female MEF-CiPSCs. (E) Scatterplots of genome-wide transcription. Parallel diagonal lines indicate the two-fold threshold in expression difference (p < 0.05).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Zscan4 Millipore Cat#: AB4340 Pan-Kcr PTM biolabs Cat#: PTM-502 Oct4 Santa Cruz Cat#: sc5279 b-Actin Abmart Cat#: P30002 Ki67 Millipore Cat#: AB9260 LaminA Abcam Cat#: ab26300 Foxl2 Abcam Cat#: ab5096 H3K27me3 Millipore Cat#: 07-499 Sox2 Millipore Cat#: AB5603 Nanog Abcam Cat#: ab80892 Lin28 CST Cat#: 3978S bIII-tubulin Chemicon Cat#: CBL412 a-SMA Abcam Cat#: ab5694 AFP Dako Cat#: DAK-N1501 Nestin Millipore Cat#: MAB353 a-Tubulin Sigma Cat#: SAB4800087 SCP1 Abcam Cat#: ab15090 SCP3 Novus Cat#: NB300-230 MLH1 BD Cat#: 550838 Texas Red-conjugated Phalloidin Abcam Cat#: ab176757 anti-SSEA1 conjugated with magnetic beads Miltenyi Cat#: 130-094-530 anti-SSEA1 conjugated with Alexa Fluor 647 eBioscience Cat#: 50-8813-42 anti-CD61 conjugated with PE BioLegend Cat#: 104307 FITC Goat Anti-Mouse IgG (H+L) Jackson Cat#: 115-095-003 Alexa Fluor 594 Goat Anti-Rabbit IgG (H+L) Jackson Cat#: 111-585-003 Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) Life Cat#: A11008 Alexa Fluor 594 Goat Anti-Mouse IgG (H+L) Life Cat#: A-11005 Alexa Fluor 594 Donkey Anti-Goat IgG (H+L) Abcam Cat#: ab150132 Goat anti-Rabbit IgG-HRP GE Healthcare Cat#: NA934V Goat anti-Mouse IgG (H+L)/HRP ZSGB-BIO Cat#: ZB2305 Bacterial and Virus Strains Trans1-T1 Phage Resistant Chemically Competent Cell Transgene Cat#: CD501-03 Chemicals, Peptides, and Recombinant Proteins Y27632 Selleck Cat#: S1049 PD0325901 Miltenyi Cat#: 04-0006 CHIR99021 Selleck Cat#: S1263 ESGRO (LIF) Millipore Cat#: ESG1107 Human bFGF PeproTech Cat#: 96-100-18B VPA Sigma Cat#: P4543 Repsox Selleck Cat#: S7223 Parnate Sigma Cat#: P8511 Forskolin Selleck Cat#: S2449 AM580 Tocris Cat#: 0760-10 (Continued on next page) e1 Cell Reports 29, 4256–4267.e1–e9, December 24, 2019

Techniques: Expressing, Methylation, Injection, Transmission Assay, Genome Wide

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

doi: 10.12659/MSM.912109

Figure Lengend Snippet: POU5F1B controls the expression of OCT4. ( A ) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the mRNA levels of OCT4 after transfected with si-POU5F1B or si-NC. The data are represented as the mean ±SD. * P<0.05. ( B ) Western blot analysis was performed to determine the OCT4 protein expression after transfection with si-POU5F1B or si-NC.

Article Snippet: The membranes were blocked with 5% dried skimmed milk powder for an hour and incubated in primary antibodies to OCT4 (Boster, Wuhan, China) at 4°C overnight and GAPDH (Beyotime, Haimen, China) at 4°C overnight.

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Western Blot