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ocelloscope  (Unisensor)
90
Unisensor ocelloscope
Cell morphology of MG1655/CTX-M-1, MG1655/IncI1/CTX-M-1, MG1655/NCS and MG1655/IncI1/NCS cultured with and without cefotaxime. (A) Filmentation at different cefotaxime concentrations. Pictures are taken in early exponential phase, except where stated otherwise. (1–5) MG1655/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 32 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. (6–10) MG1655/IncI1/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 64 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. Picture (5) and (10) were taken in late stationary phase. (11–12) MG1655/NCS cultured without cefotaxime and with 0.016 mg/L cefotaxime, respectively. (13–14) MG1655/IncI1/NCS cultured without cefotaxime and with 0.032 mg/L cefotaxime, respectively. (B) Filamentation and growth phase dependency. The growth curves are representative drawings, where the morphology of the cells (resistant or sensitive) is marked at different growth phases. The strains were grown in MH-2 broth with and without cefotaxime on an <t>oCelloScope.</t>
Ocelloscope, supplied by Unisensor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell morphology of MG1655/CTX-M-1, MG1655/IncI1/CTX-M-1, MG1655/NCS and MG1655/IncI1/NCS cultured with and without cefotaxime. (A) Filmentation at different cefotaxime concentrations. Pictures are taken in early exponential phase, except where stated otherwise. (1–5) MG1655/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 32 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. (6–10) MG1655/IncI1/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 64 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. Picture (5) and (10) were taken in late stationary phase. (11–12) MG1655/NCS cultured without cefotaxime and with 0.016 mg/L cefotaxime, respectively. (13–14) MG1655/IncI1/NCS cultured without cefotaxime and with 0.032 mg/L cefotaxime, respectively. (B) Filamentation and growth phase dependency. The growth curves are representative drawings, where the morphology of the cells (resistant or sensitive) is marked at different growth phases. The strains were grown in MH-2 broth with and without cefotaxime on an oCelloScope.

Journal: BMC Microbiology

Article Title: Extended spectrum β-lactamase-producing Escherichia coli forms filaments as an initial response to cefotaxime treatment

doi: 10.1186/s12866-015-0399-3

Figure Lengend Snippet: Cell morphology of MG1655/CTX-M-1, MG1655/IncI1/CTX-M-1, MG1655/NCS and MG1655/IncI1/NCS cultured with and without cefotaxime. (A) Filmentation at different cefotaxime concentrations. Pictures are taken in early exponential phase, except where stated otherwise. (1–5) MG1655/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 32 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. (6–10) MG1655/IncI1/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 64 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. Picture (5) and (10) were taken in late stationary phase. (11–12) MG1655/NCS cultured without cefotaxime and with 0.016 mg/L cefotaxime, respectively. (13–14) MG1655/IncI1/NCS cultured without cefotaxime and with 0.032 mg/L cefotaxime, respectively. (B) Filamentation and growth phase dependency. The growth curves are representative drawings, where the morphology of the cells (resistant or sensitive) is marked at different growth phases. The strains were grown in MH-2 broth with and without cefotaxime on an oCelloScope.

Article Snippet: Bacterial morphology was investigated by performing growth experiments on an oCelloScope (Unisensor, Alleroed, Denmark) for 24 hours at 37°C (no shaking).

Techniques: Cell Culture