Journal: Nucleic Acids Research

Article Title: Functional transitions of the Aspergillus fumigatus iron regulator HapX are governed by conserved domains cooperatively binding [2Fe-2S] clusters

doi: 10.1093/nar/gkaf796

Figure Lengend Snippet: In liquid media, growth inhibition by combinatorial mutation of at least CRR-B and -C is more severe in +Fe compared to −Fe and accelerates with increased expression of the corresponding hapX alleles. For each strain, ( A ) pxylP:hapX , ( B ) pxylP:hapX A/B/C/D , ( C ) pxylP:hapX B/C , and ( D ) pxylP:hapX A/B/C/D,464 , 10 4 conidia were inoculated in triplicate into 96-well microplates in 0.1 mL minimal medium reflecting iron limitation (−Fe; in green) and iron sufficiency (+Fe; 10 μM FeSO 4 ; in red) supplemented with 0.1% xylose for moderate and 1% xylose for high expression of the respective hapX allele. Using the oCelloScope (BioSense Solutions, Denmark) and its UniExplorer software (version 14.0), growth was scored hourly at 37 °C from 2 h post-inoculation (graphs start at the 5 h time point as there was very limited growth before that) until 24 h incubation. In the software, the “ fungi filamentous growth module ” was used to assess the hyphal area up to 2.75 × 10 5 μm 2 when the growth measurement reached saturation. The values in the growth curves represent the mean ± standard deviation of biological triplicates. Below the growth curves are examples of microscopic images of the fungal cultures taken automatically by the oCelloScope at two time points, 12 h and 24 h, underlining the scored growth data.

Article Snippet: Therefore, we applied automated microbial live cell imaging and analysis using the oCelloScope (BioSense Solutions ApS, Denmark) measuring growth by determining hyphal area (Fig. ).

Techniques: Inhibition, Mutagenesis, Expressing, Software, Incubation, Standard Deviation

Journal: BMC Microbiology

Article Title: Extended spectrum β-lactamase-producing Escherichia coli forms filaments as an initial response to cefotaxime treatment

doi: 10.1186/s12866-015-0399-3

Figure Lengend Snippet: Cell morphology of MG1655/CTX-M-1, MG1655/IncI1/CTX-M-1, MG1655/NCS and MG1655/IncI1/NCS cultured with and without cefotaxime. (A) Filmentation at different cefotaxime concentrations. Pictures are taken in early exponential phase, except where stated otherwise. (1–5) MG1655/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 32 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. (6–10) MG1655/IncI1/CTX-M-1 cultured without cefotaxime, with 4 mg/L cefotaxime, with 16 mg/L cefotaxime, with 64 mg/L cefotaxime and with 256 mg/L cefotaxime, respectively. Picture (5) and (10) were taken in late stationary phase. (11–12) MG1655/NCS cultured without cefotaxime and with 0.016 mg/L cefotaxime, respectively. (13–14) MG1655/IncI1/NCS cultured without cefotaxime and with 0.032 mg/L cefotaxime, respectively. (B) Filamentation and growth phase dependency. The growth curves are representative drawings, where the morphology of the cells (resistant or sensitive) is marked at different growth phases. The strains were grown in MH-2 broth with and without cefotaxime on an oCelloScope.

Article Snippet: Bacterial morphology was investigated by performing growth experiments on an oCelloScope (Unisensor, Alleroed, Denmark) for 24 hours at 37°C (no shaking).

Techniques: Cell Culture