Journal: Marine Drugs

Article Title: Anti-Inflammatory Effects of Marine-Derived Resorcylic Acid Lactone Derivatives in Ulcerative Colitis via the MAPK/ERK Pathway

doi: 10.3390/md24020069

Figure Lengend Snippet: Compound 4 exhibits potent in vivo anti-inflammatory activity in DSS-induced colitis. ( A ) Schematic diagram of colitis. C57BL/6 mice were given 3% DSS water for 8 days. 5-ASA (100 mg/kg) and compound 4 (10, 20 mg/kg) were administered daily by gavage for 9 consecutive days, and the mice were sacrificed on day 9. ( B ) At the end of the experiment, the representative results of colon length. The weight ( C ) and DAI score ( D ) of mice in each group were recorded daily. ( E ) spleen index of each group. ( F ) Histopathological images of the colon in each group of mice. Scale bar = 100 µm. ( G ) Expression levels of ZO-1, Occludin, and Claudin-1 in colon tissues of each group. ( H – K ) The concentration of LPS, TNF- α , IL-6, and IL-1 β in the serum of each group. The samples were derived from the same experiment, and the blots were processed in parallel. Data represent mean ± SD, ns: not significant; ## p < 0.01 vs. control group; ** p < 0.01 vs. DSS group.

Article Snippet: After blocking with 5% BSA for 2 h, the membranes were incubated at 4 °C overnight with primary antibodies against the following target proteins: β -Actin (CST, #4970), P-ERK (CST, #5726), ERK (CST, #4695), ZO-1 (Affinity, #AF5145), Occludin (Proteintech, #27260-1-AP), and Claudin-1 (Proteintech, #28674-1-AP).

Techniques: In Vivo, Activity Assay, Expressing, Concentration Assay, Derivative Assay, Control

Journal: Frontiers in psychiatry

Article Title: Evaluation of serum zonulin and occludin levels in obsessive-compulsive disorder and the effect of major depressive disorder comorbidity.

doi: 10.3389/fpsyt.2024.1395235

Figure Lengend Snippet: FIGURE 2 Dot Plots Showing The Goups’ Serum Zonulin and Occludin Levels According to Y-BOCS and HDRS Scores.

Article Snippet: Zonulin and occludin levels were measured with the ELISA method using a Human Zonulin ELISA kit (Catalog No. EELH5560; Elabscience Biotechnology Inc., Houston, TX, USA) and a Human Occludin ELISA kit (Catalog No. E-ELH1073; Elabscience Biotechnology Inc.) in line with the manufacturer’s instructions.

Techniques:

Journal: Frontiers in psychiatry

Article Title: Evaluation of serum zonulin and occludin levels in obsessive-compulsive disorder and the effect of major depressive disorder comorbidity.

doi: 10.3389/fpsyt.2024.1395235

Figure Lengend Snippet: FIGURE 4 Scatter-Plots Between Serum Zonulin and Occludin Levels and Y-BOCS and HDRS Scores.

Article Snippet: Zonulin and occludin levels were measured with the ELISA method using a Human Zonulin ELISA kit (Catalog No. EELH5560; Elabscience Biotechnology Inc., Houston, TX, USA) and a Human Occludin ELISA kit (Catalog No. E-ELH1073; Elabscience Biotechnology Inc.) in line with the manufacturer’s instructions.

Techniques: