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Image Search Results
Journal: Translational Cancer Research
Article Title: Contribution of sphingomyelin phosphodiesterase acid-like 3B to the proliferation, migration, and invasion of ovarian cancer cells
doi: 10.21037/tcr-24-309
Figure Lengend Snippet: SMPDL3B promoted the proliferation of ovarian cancer cells. (A) qRT-PCR assay for measuring the mRNA production of SMPDL3B gene in the Caov-3, SK-OV-3, and HEY cell lines; n=3. (B,C) Western blot analysis of SMPDL3B protein levels following shRNA knockdown in the SK-OV-3 and HEY cell lines; n=3. (D,E) CCK-8 cell proliferation assay assessing the impact of SMPDL3B knockdown on the proliferation of the SK-OV-3 and HEY cell lines; n=3. *, P<0.05; ****, P<0.0001. SMPDL3B , sphingomyelin phosphodiesterase acid-like 3B; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, cell count kit-8; OD, optical density.
Article Snippet: The human ovarian carcinoma cell lines used in our study included the following: Caov-3 cells [cat. no. CTCC-400-0069; China Center for Type Culture Collection (CCTCC), Wuhan, China], characterized as human poorly differentiated ovarian clear cell carcinoma cells; SK-OV-3 cells (cat. no. CTCC-001-0011; CCTCC); and
Techniques: Quantitative RT-PCR, Western Blot, shRNA, Knockdown, CCK-8 Assay, Proliferation Assay, Real-time Polymerase Chain Reaction, Cell Counting
Journal: Oncology Letters
Article Title: LINC00473 modulates protein expression to promote ovarian cancer progression and overcome cisplatin resistance
doi: 10.3892/ol.2026.15456
Figure Lengend Snippet: LINC00473 knockdown efficiency in ovarian cancer cell lines. Quantitative analysis of LINC00473 expression in (A) SK-OV-3 and (B) A2780 cells after transfection with siLINC00473, compared with siNC and untreated controls. Data are presented as the mean±SD. ***P<0.001. LINC, long non-coding RNA; NC, negative control; si, small interfering RNA.
Article Snippet: SK-OV-3 and
Techniques: Knockdown, Expressing, Transfection, Negative Control, Small Interfering RNA
Journal: Oncology Letters
Article Title: LINC00473 modulates protein expression to promote ovarian cancer progression and overcome cisplatin resistance
doi: 10.3892/ol.2026.15456
Figure Lengend Snippet: Silencing LINC00473 significantly inhibits cell proliferation and induces apoptosis. Proliferation of (A) SK-OV-3 and (B) A2780 cells after transfection with siLINC00473, siNC and untreated controls (C). Proliferation inhibition rates of both cell lines post-transfection. Apoptosis status of (D) SK-OV-3 and (E) A2780 cells. Data are presented as the mean±SD. ***P<0.001. LINC, long non-coding RNA; NC, negative control; si, small interfering RNA.
Article Snippet: SK-OV-3 and
Techniques: Transfection, Inhibition, Negative Control, Small Interfering RNA
Journal: Oncology Letters
Article Title: LINC00473 modulates protein expression to promote ovarian cancer progression and overcome cisplatin resistance
doi: 10.3892/ol.2026.15456
Figure Lengend Snippet: Cellular protein expression after siLINC00473 transfection. (A) Differential expression patterns of five proteins in two ovarian cancer cell lines after transfection with siLINC00473 vs. the scrambled siNC. Semi-quantitative analysis of relative expression levels of (B) PARP, (C) caspase-3, (D) Sox2 and (E) YAP1 normalized to GAPDH, demonstrating significant downregulation in siLINC00473-treated cells compared with siNC. Data are presented as the mean±SD; ***P<0.001. LINC, long non-coding RNA; NC, negative control; PARP, poly (adenosine diphosphate-ribose) polymerase; si, small interfering RNA; YAP1, yes-associated protein 1.
Article Snippet: SK-OV-3 and
Techniques: Expressing, Transfection, Quantitative Proteomics, Negative Control, Small Interfering RNA
Journal: Oncology Letters
Article Title: LINC00473 modulates protein expression to promote ovarian cancer progression and overcome cisplatin resistance
doi: 10.3892/ol.2026.15456
Figure Lengend Snippet: Effect of siLINC00473 transfection on cellular sensitivity to CDDP. Comparison of cellular sensitivity to CDDP after transfection of (A) SK-OV-3 and (B) A2780 cells with siLINC00473, compared with the siNC group and the blank control group. ***P<0.001. LINC, long non-coding RNA; CDDP, cisplatin; NC, negative control; si, small interfering RNA.
Article Snippet: SK-OV-3 and
Techniques: Transfection, Comparison, Control, Negative Control, Small Interfering RNA