Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation

doi: 10.1177/10406387211044192

Figure Lengend Snippet: Conventional Bactec MGIT (MGIT tube containing PANTA-OADC antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and TiKa supplement B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A. Mycobacterium bovis SB0121 (10,000–10 cfu) and B. M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t -test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance ( p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium , bars with a different superscript are significantly different at p < 0.01.

Article Snippet: TiKa liquid cultures used 7-mL MGIT tubes containing 800 μL of antimicrobial growth supplement mixture (PANTA + OADC; Becton Dickinson), 8.5 μL of growth supplement B (TiKa Diagnostics), and 500 μL of each treated sample.

Techniques: Sterility, Control

Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation

doi: 10.1177/10406387211044192

Figure Lengend Snippet: Comparative mycobacterial culture results for tissue and respiratory samples (bronchial alveolar lavage fluid and trunk washes) collected from 26 known Mycobacterium tuberculosis complex (MTBC)-infected African buffaloes, white rhinoceros (25 samples from 6 animals), and African elephants (3 samples from 2 animals) from bovine tuberculosis endemic wildlife parks and a zoo (1 elephant) within South Africa.

Article Snippet: TiKa liquid cultures used 7-mL MGIT tubes containing 800 μL of antimicrobial growth supplement mixture (PANTA + OADC; Becton Dickinson), 8.5 μL of growth supplement B (TiKa Diagnostics), and 500 μL of each treated sample.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Decontamination Effect of Hypochlorous Acid Dry Mist on Selected Bacteria, Viruses, Spores, and Fungi as Well as on Components of Electronic Systems

doi: 10.3390/ijms25137198

Figure Lengend Snippet: List of strains of bacteria, fungi, and corresponding media, incubation conditions, as well as the number of test microorganisms obtained in the initial suspension, and environmental parameters at the beginning of the disinfection process.

Article Snippet: Mycobacterium avium , Middlebrook Agar z supplement (OADC)/(Becton Dickson GmbH, Heidelberg, Germany) , 37 °C/21 days, increased humidity conditions , 1.25 × 10 8 , 64.8 , 21.3.

Techniques: Bacteria, Incubation, Suspension

Journal: Scientific Reports

Article Title: The influence of AccD5 on AccD6 carboxyltransferase essentiality in pathogenic and non-pathogenic Mycobacterium

doi: 10.1038/srep42692

Figure Lengend Snippet: ( A ) Growth in 7H9/OADC medium with (solid line) and without (dashed line) acetamide was followed over time by measuring the OD 600 nm (top) and number of viable cells (bottom). Values are means ± standard errors from three independent experiments. ( B) (left) Images of the mutant liquid cultures after 24 h of growth with (+) and without (−) acetamide. (right) Mutant growth on 7H10/OADC agar with (+) and without (−) acetamide. ( C) (top) Monitoring of the accD4 Msm expression level. The accD4 Msm transcript level was measured in mutant strain growing for 12 and 24 h in 7H9/OADC medium with (+) and without (−) acetamide. The expression level of the same transcript was also measured in the ∆ accD6 parental strain after 24 h of culture. (bottom) Thin-layer chromatography of 14 C-labeled fatty acid methyl esters (FAMEs) and mycolic acid methyl esters (MAMEs) extracted from the mutant after 3, 12 and 24 h of culture in 7H9/OADC medium with (+) and without (−) acetamide. Equal counts (10 000 cpm) were loaded onto TLC plate and developed in hexane – ethyl acetate (19:1, vol/vol). The symbols: α, α′, and e correspond to α-mycolates, α′-mycolates and epoxy-mycolates, respectively.

Article Snippet: Strains based on M. tuberculosis and M. smegmatis were maintained on Middlebrook 7H10 agar or 7H9 broth (Becton Dickinson) with 10% OADC (oleic acid, albumin, dextrose, catalase) enrichment (Becton Dickinson).

Techniques: Mutagenesis, Expressing, Thin Layer Chromatography, Labeling