o 2 Search Results


o2  (Isotec Inc)
86
Isotec Inc o2
O2, supplied by Isotec Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pm29120636-28-27-7?v=Isotec+Inc
Average 86 stars, based on 1 article reviews
o2 - by Bioz Stars, 2026-08
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g418  (Addgene inc)
93
Addgene inc g418
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
G418, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pm37828515-78-6-34?v=Addgene+inc
Average 93 stars, based on 1 article reviews
g418 - by Bioz Stars, 2026-08
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acetamido 2 deoxy d glucopyranosylidene amino n phenylcarbamate pugnac  (Toronto Research Chemicals)
93
Toronto Research Chemicals acetamido 2 deoxy d glucopyranosylidene amino n phenylcarbamate pugnac
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Acetamido 2 Deoxy D Glucopyranosylidene Amino N Phenylcarbamate Pugnac, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pmc04628934-188-83-91?v=Toronto+Research+Chemicals
Average 93 stars, based on 1 article reviews
acetamido 2 deoxy d glucopyranosylidene amino n phenylcarbamate pugnac - by Bioz Stars, 2026-08
93/100 stars
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multichannel o2 meter  (PreSens Inc)
94
PreSens Inc multichannel o2 meter
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Multichannel O2 Meter, supplied by PreSens Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pm37916608-135-22-27?v=PreSens+Inc
Average 94 stars, based on 1 article reviews
multichannel o2 meter - by Bioz Stars, 2026-08
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anti cathepsin k ctsk antibody  (Proteintech)
95
Proteintech anti cathepsin k ctsk antibody
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Anti Cathepsin K Ctsk Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pmc12908679-33-32-38?v=Proteintech
Average 95 stars, based on 1 article reviews
anti cathepsin k ctsk antibody - by Bioz Stars, 2026-08
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acetamido 2 deoxy d glucopyranosylidene amino n phenyl carbamate pugnac  (Toronto Research Chemicals)
86
Toronto Research Chemicals acetamido 2 deoxy d glucopyranosylidene amino n phenyl carbamate pugnac
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Acetamido 2 Deoxy D Glucopyranosylidene Amino N Phenyl Carbamate Pugnac, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pmc05448127-524-1-12?v=Toronto+Research+Chemicals
Average 86 stars, based on 1 article reviews
acetamido 2 deoxy d glucopyranosylidene amino n phenyl carbamate pugnac - by Bioz Stars, 2026-08
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fluvoxamine  (Toronto Research Chemicals)
93
Toronto Research Chemicals fluvoxamine
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Fluvoxamine, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pmc04109210-256-0-4?v=Toronto+Research+Chemicals
Average 93 stars, based on 1 article reviews
fluvoxamine - by Bioz Stars, 2026-08
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sar9 met o2 11 substance p sp  (Tocris)
94
Tocris sar9 met o2 11 substance p sp
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Sar9 Met O2 11 Substance P Sp, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pm33927636-66-4-20?v=Tocris
Average 94 stars, based on 1 article reviews
sar9 met o2 11 substance p sp - by Bioz Stars, 2026-08
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13c6 102 thioglucose  (Toronto Research Chemicals)
91
Toronto Research Chemicals 13c6 102 thioglucose
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
13c6 102 Thioglucose, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/chem_rxiv___2021___cvvgp-37-112-123?v=Toronto+Research+Chemicals
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13c6 102 thioglucose - by Bioz Stars, 2026-08
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acetamido2 deoxy d glucopyranosylidene amino z nphenylcarbamate pugnac  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology acetamido2 deoxy d glucopyranosylidene amino z nphenylcarbamate pugnac
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
Acetamido2 Deoxy D Glucopyranosylidene Amino Z Nphenylcarbamate Pugnac, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pmc05674511__SC___008___C7SC01966A___s001-10-0-6?v=Santa+Cruz+Biotechnology
Average 90 stars, based on 1 article reviews
acetamido2 deoxy d glucopyranosylidene amino z nphenylcarbamate pugnac - by Bioz Stars, 2026-08
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m6am  (Toronto Research Chemicals)
93
Toronto Research Chemicals m6am
Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under <t>G418</t> selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information
M6am, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o+2/pm34431664-706-39-40?v=Toronto+Research+Chemicals
Average 93 stars, based on 1 article reviews
m6am - by Bioz Stars, 2026-08
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Image Search Results


Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under G418 selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information

Journal: Journal of translational medicine

Article Title: Dissecting transcription of the 8q24-MYC locus in prostate cancer recognizes the equilibration between androgen receptor direct and indirect dual-functions.

doi: 10.1186/s12967-023-04429-4

Figure Lengend Snippet: Fig. 4 CRISPR/Cas9-mediated genomic knock-out (KO) of P10 to evaluate its impact on androgen-elicited MYC transrepression. A A schematic showing of the MYC gene locus with the P10-KO strategy. Highlighted are VCaP AR ChIP-Seq tracks, two pairs of P10 targeting gRNA (U2/U1 for upstream gRNAs under G418 selection; and D1/D2 for downstream gRNAs under Puro selection), together with PCR and DNA-Seq primers. B Genomic DNA of stable pools were amplified by round-1 PCR. The predicted PCR product sizes: control pool (995 bp) and P10-KO pools (371 bp, 317 bp, 313 bp, and 259 bp). C The round-1 PCR products were re-amplified by round-2 PCR (nest-PCR). The predicted PCR product sizes: control pool (941 bp), P10-KO pools (316 bp, 262 bp, 258 bp, and 204 bp). D as loading controls, control PCR tests were conducted with indicated primers to amplify the MYC and PSA loci that are not targeted by gRNA. Arrow heads indicated bands with expected sizes. E The identities of the major nest-PCR bands were assessed and verified by band excision, DNA purification, DNA-Seq, and alignment. The results indicated that editing predominantly occurred at U1 and D2 gRNA sites. F Control (C) and P10-KO pools in androgen-depleted medium were subjected to 10 nM of DHT treatment for indicated time points. TaqMan RT-qPCR was conducted on MYC mRNA expression (GAPDH as internal reference). The normalized read-outs were shown, with the vehicle-treated control samples being set at 1. Data are mean values ± SD for three biologically independent samples. P values are two-sided Student’s t test. *P < 0.05; **P < 0.01. See Additional file 1: Fig. S4, S5 for additional information

Article Snippet: DHT (S4757, SelleckChem, Houston, TX, USA), G418 (10131035, Thermo Fisher, Waltham, MA, USA), Puromycin (NC9138068, Fishersci, Hampton, NH, USA), Hygromycin B (Thermo Fisher, 10687010), Blasticidin (Thermo Fisher, A1113903), Lenti-gRNA-Neo (G418) vector (LGN; Catalog. 104992, Addgene, Watertown, MA, USA) and Lenti-gRNA-Puro vector (LGP; Addgene, Catalog. 104990).

Techniques: CRISPR, Knock-Out, ChIP-sequencing, Selection, DNA Sequencing, Amplification, Control, DNA Purification, Quantitative RT-PCR, Expressing