Journal: PLoS ONE

Article Title: Loss of the V-ATPase B1 Subunit Isoform Expressed in Non-Neuronal Cells of the Mouse Olfactory Epithelium Impairs Olfactory Function

doi: 10.1371/journal.pone.0045395

Figure Lengend Snippet: (A) A schematic diagram showing the subunit composition of the V-ATPase. The cytosolic V 1 domain is composed of subunits A through H (shown in white or light gray, marked with blue letters). The transmembrane V 0 domain is composed of subunits a, c, c” (or b), d, e, and Ac45 (shown in blue, marked with white letters). Some of the subunit interactions are putative. (B) Section from a 3-D image reconstruction showing that B1 V-ATPase (red) localizes to the microvilli of olfactory sustentacular cells in a 2-week old female mouse pup. Apical cilia of olfactory sensory neurons are immunostained for CNGA2 (green). DAPI (blue) stains cell nuclei. Bar = 30 µm.

Article Snippet: We used the following commercially available antibodies as olfactory markers: a goat polyclonal antibody against olfactory marker protein (OMP) (WAKO Chemicals USA, Richmond, VA), a rabbit polyclonal antibody against cytokeratin-18 (CK-18) (Abcam, Cambridge, MA), a rabbit polyclonal antibody against cyclic nucleotide gated cation channel 2 (CNGA2) (Alomone Labs, Jerusalem, Israel), a mouse monoclonal anti-tubulin antibody and a rat monoclonal anti-L-CAM/E-cadherin antibody (Sigma-Aldrich, St. Louis, MO).

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