Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: The bar graph represents mean values of the indicated miRNAs in plasma of DM1 patients compared to controls (CTR). Average values of cel-miR-39, miR106a and 17-5p were used for normalization (DM1 n = 103, CTR n = 111; ****p < 0.0001, *p < 0.05).

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: ( a ) Box plot of miR-133b levels in the plasma of DM1 and controls (CTR; ****p < 0.0001). ( b ) ROC curve illustrating sensitivity and specificity of plasma miR-133b in discriminating healthy from diseased patients. ( c ) Spearman’s inverse correlation between miRNA-133b fold change compared to CTR and muscle strength measured by MRC megascore. ( d ) Spearman’s direct correlation between miRNA-133b fold change compared to CTR and CK plasma levels. For all panels, DM1 n = 103, CTR n = 111.

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: The DM1-miRNA score was calculated averaging the fold changes of miR-1, -133a, -133b, -140-3p, -206, -27b, -454 and -574 in the plasma of DM1 and controls (DM1 n = 103, CTR n = 111). ( a ) Box plot of DM1-miRNA score levels in the plasma of DM1 and controls (CTR; ****p < 0.0001). ( b ) ROC curve illustrating sensitivity and specificity of the DM1-miRNA score in discriminating healthy from diseased patients. ( c ) Spearman’s inverse correlation between the DM1-miRNA score and muscle strength measured by MRC megascore. ( d ) Spearman’s direct correlation between the DM1-miRNA score and CK plasma levels.

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: The miR-133a/b score was calculated averaging the fold changes of miRNA-133a and -133b in the plasma of DM1 and controls (DM1 n = 103, CTR n = 111). ( a ) Box plot of miR-133a/b score levels in the plasma of DM1 and controls (CTR; ****p < 0.0001). ( b ) ROC curve illustrating sensitivity and specificity of the miR-133a/b score in discriminating healthy from diseased patients. ( c ) Spearman’s inverse correlation between the miR-133a/b score and muscle strength measured by MRC megascore. ( d ) Spearman’s direct correlation between the miR-133a/b score and CK plasma levels.

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: Multiple regression analysis, DM1 patients with stage of disease higher than 2.

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques:

Journal: Scientific Reports

Article Title: Validation of plasma microRNAs as biomarkers for myotonic dystrophy type 1

doi: 10.1038/srep38174

Figure Lengend Snippet: ( a ) The bar graph represents mean values of the indicated miRNAs in plasma of DM2 patients compared to controls (CTR). Average values of cel-miR-39, miR106a and 17-5p were used for normalization (****p < 0.0001, ***p < 0.001, **p < 0.01,*p < 0.05). ROC curves illustrating sensitivity and specificity of DM2-miRNA score ( b ), myomiR ( c ) and miR-133a/b score ( d ) in discriminating healthy from disease patients. (DM2 n = 30, CTR n = 30).

Article Snippet: Total RNA was extracted as previously described using NucleoSpin miRNA Plasma columns (Macherey-Nagel) .

Techniques: Clinical Proteomics