ntm Search Results


gene exp ntm rn01525004 m1  (Thermo Fisher)
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Thermo Fisher gene exp ntm rn01525004 m1
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iglon2  (OriGene)
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OriGene iglon2
Membrane proteins identified as bona-fide IgLON5 interactors.
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iglon2  (Proteintech)
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Proteintech iglon2
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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gene exp ntm mm00549431 m1  (Thermo Fisher)
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Thermo Fisher gene exp ntm mm00549431 m1
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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ntm: zeitschrift für ge- schichte der wissenschaften, technik und medizin  (Technik GmbH)
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Technik GmbH ntm: zeitschrift für ge- schichte der wissenschaften, technik und medizin
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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genotype mycobacterium cm/ntm-dr  (Hain Lifescience)
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Hain Lifescience genotype mycobacterium cm/ntm-dr
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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advansure rv real-time pcr kit  (LG Life)
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LG Life advansure rv real-time pcr kit
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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real-time pcr real-q mtb & ntm  (BioSewoom Inc)
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FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
Real Time Pcr Real Q Mtb & Ntm, supplied by BioSewoom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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broth microdilution method brothmic rgm  (Kyokuto Pharmaceutical Industrial Co Ltd)
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FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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broth mic ntm  (Kyokuto Pharmaceutical Industrial Co Ltd)
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FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
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genotype ntm-dr kits  (Hain Lifescience)
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Hain Lifescience genotype ntm-dr kits
Comparison of gene sequencing methods and <t> GenoType </t> NTM-DR for the identification of 173 Mycobacterium avium complex species
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tb/ntm kit detection test  (CapitalBio Corporation)
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Comparison of gene sequencing methods and <t> GenoType </t> NTM-DR for the identification of 173 Mycobacterium avium complex species
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Image Search Results


Membrane proteins identified as bona-fide IgLON5 interactors.

Journal: Frontiers in Immunology

Article Title: Patients’ IgLON5 autoantibodies interfere with IgLON5-protein interactions

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: Membrane proteins identified as bona-fide IgLON5 interactors.

Article Snippet: To validate cis -interactions (within the same cell) of IgLON5 with identified interactors, HEK cells were co-transfected in pairs: IGLON5 (SC317071, Origene) plus: 1) IGLON1 (RG213594, Origene), 2) IGLON2 (RG226879, Origene), 3) IGLON3 (RG207618, Origene), 4) IGLON4 (RG216034 Origene), 5) KIDINS220 (RC216441Origene), 6) IGSF21 (RG203559, Origene), 7) CACNA2D2 (RC219452, Origene) and 8) CASPR2 (SC115167, Origene), as a non-binding control.

Techniques: Membrane

Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Journal: Frontiers in Immunology

Article Title: Patients’ IgLON5 autoantibodies interfere with IgLON5-protein interactions

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Article Snippet: To validate cis -interactions (within the same cell) of IgLON5 with identified interactors, HEK cells were co-transfected in pairs: IGLON5 (SC317071, Origene) plus: 1) IGLON1 (RG213594, Origene), 2) IGLON2 (RG226879, Origene), 3) IGLON3 (RG207618, Origene), 4) IGLON4 (RG216034 Origene), 5) KIDINS220 (RC216441Origene), 6) IGSF21 (RG203559, Origene), 7) CACNA2D2 (RC219452, Origene) and 8) CASPR2 (SC115167, Origene), as a non-binding control.

Techniques: Western Blot, Immunoprecipitation, Transfection, Incubation

FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Journal: Frontiers in immunology

Article Title: Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions.

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Article Snippet: The presence of the interacting proteins was detected by incubating the membrane with the following antibodies (dilution 1:500, overnight at 4°C): IgLON1 (STJ94590), IgLON2 (STJ94442), IgLON3 (STJ116461), IgLON4 (STJ94394, St. John’s Laboratory, London, UK), KIDINS220 (21856-1-AP, Proteintech, Rosemont, IL, USA), IGSF21 (21465-1-AP; Proteintech), CACNA2D2 (SAB1401461, SIGMA) and CASPR2 (ab33994, Abcam) followed by incubation with appropriate secondary antibodies (dilution 1:1000) for 1 hour at room temperature.

Techniques: Western Blot, Immunoprecipitation, Transfection, Incubation

Comparison of gene sequencing methods and GenoType NTM-DR for the identification of 173 Mycobacterium avium complex species

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of GenoType NTM-DR Assay for Identification of Mycobacterium chimaera

doi: 10.1128/JCM.00009-17

Figure Lengend Snippet: Comparison of gene sequencing methods and GenoType NTM-DR for the identification of 173 Mycobacterium avium complex species

Article Snippet: We also thank Hain Lifescience for reducing the cost of GenoType NTM-DR kits used in this study.

Techniques: Comparison, Sequencing