Journal:

Article Title: Tumorigenic Role of Orphan Nuclear Receptor NR0B1 in Lung Adenocarcinoma

doi: 10.2353/ajpath.2009.090010

Figure Lengend Snippet: High NR0B1 expression in SP of A549. A: Dot blot analysis of A549 cells stained with Hoechst 33342 dye in the absence (left) or presence (right) of verapamil. SP and MP cells were boxed. B: Quantitative real-time RT-PCR was performed with mRNA obtained from SP and MP of A549. The amount of NR0B1 mRNA was normalized for the amount of GAPDH mRNA. The values represent the mean ± SE of three experiments. *P < 0.01 by the Student’s t-test.

Article Snippet: The human lung adenocarcinoma cell lines A549 and PC-14 were purchased from the American Type Culture Collection (Rockville, MD) and the Riken Bioresource Center (Tsukuba, Japan), respectively.

Techniques: Expressing, Dot Blot, Staining, Quantitative RT-PCR

Journal:

Article Title: Tumorigenic Role of Orphan Nuclear Receptor NR0B1 in Lung Adenocarcinoma

doi: 10.2353/ajpath.2009.090010

Figure Lengend Snippet: Expression of apoptosis- and invasion-related genes in NR0B1-knocked down A549 cells. A: Semiquantitative RT-PCR. B: Real-time quantitative RT-PCR to examine the expression level of Bcl-2 and MMP-2. The value is shown as the mean ± SE. *P < 0.01 by the Student’s t-test.

Article Snippet: The human lung adenocarcinoma cell lines A549 and PC-14 were purchased from the American Type Culture Collection (Rockville, MD) and the Riken Bioresource Center (Tsukuba, Japan), respectively.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Pharmaceuticals

Article Title: Metformin Enhances TKI-Afatinib Cytotoxic Effect, Causing Downregulation of Glycolysis, Epithelial–Mesenchymal Transition, and EGFR-Signaling Pathway Activation in Lung Cancer Cells

doi: 10.3390/ph15030381

Figure Lengend Snippet: ( A ) Cytotoxic effect of afatinib alone or in combination with metformin in H1975, HCC827, and A549 NSCLC cell lines. Cells were seeded and treated with the previously described schemes for 72 h and MTT assays were performed. Points represent the mean of 3 independent experiments by triplicate. Statistical analysis was performed through two-way ANOVA. ** p ≤ 0.01. ( B ) Combination index plots from NSCLC cell lines. Plots show the different afatinib concentrations for each cell line, in combination with metformin. We observed that the H1975 cell line had synergism with the two highest concentrations of afatinib (2 and 3 µM), the HCC827 cell line had a degree of synergy with the lowest afatinib concentration (3 nM), while a synergic effect in the three combined treatments of the A549 cell line was observed.

Article Snippet: NSCLC cell lines were treated with afatinib (LC laboratories.

Techniques: Concentration Assay

Journal: Pharmaceuticals

Article Title: Metformin Enhances TKI-Afatinib Cytotoxic Effect, Causing Downregulation of Glycolysis, Epithelial–Mesenchymal Transition, and EGFR-Signaling Pathway Activation in Lung Cancer Cells

doi: 10.3390/ph15030381

Figure Lengend Snippet: EMT biomarkers in NSCLC cell lines treated with metformin, afatinib, and the combination scheme. Cells were seeded and treated for 72 h with their respective metformin–afatinib concentrations. GAPDH was used as constitutive control, Western blot images were analyzed by image (NIH) and represented as bars. Images are representative of three independent experiments and results of area are presented as mean ± SD. For the zimogram assay, images are representative of two independent experiments. Data were normalized regarding endogenous control and statistically analyzed by one-way ANOVA. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

Article Snippet: NSCLC cell lines were treated with afatinib (LC laboratories.

Techniques: Control, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: The expression of NKILA was higher in normal human bronchial epithelial cell line. For (A) and (B), the expression level of lncRNA-NKILA was analyzed by Q-PCR. The expression of NKILA was analyzed in NSCLC tissues (C), Kaplan-Meier analyses of the correlations between lncRNA-NKILA expression level and survival (D). Values are means ± SEM for n = 7-8. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.

Article Snippet: A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Expressing, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: NKILA inhibited the proliferation and migration of NSCLC adenocarcinoma cell lines. A. The expression of NKILA after transfected with Ad-GFP or Ad-GFP-NKILA. B. MTT assay was performed after transfected with Ad-GFP or Ad-GFP-NKILA. C, D. The migration of NSCLC cells after transfected with Ad-GFP or Ad-GFP-NKILA. Values are means ± SEM for n = 7-8. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.

Article Snippet: A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Migration, Expressing, Transfection, MTT Assay, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: NKILA inhibited IL-11 mRNA expression in NSCLC cells. (A, B) The mRNA levels of IL-11 after transfected with Ad-GFP or Ad-GFP-NKILA measured by Q-PCR in different NSCLC cells. (C) The stability of IL-11 mRNA over time was measured by Q-PCR relative to time 0 after induced RNA degradation with si-NKILA (D, E) IL-11 mRNA levels were increased in a dose-dependent manner in NCI-H1975 cells with si-NKILA. Values are means 6 SEM for n = 6. *P < 0.05 vs. control.

Article Snippet: A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Expressing, Transfection, Control