Journal: ChemistryOpen

Article Title: Effects of 3‐Bromo‐4,5‐dihydroisoxazole Derivatives on Nrf2 Activation and Heme Oxygenase‐1 Expression

doi: 10.1002/open.201800185

Figure Lengend Snippet: Compound 1 is the most efficient Nrf2 and HO‐1 activator in THP‐1 cells. A) Western blot results of THP‐1 cells treated for 6 h with the different compounds. B) Densitometric analysis of Western blots. C+ is the positive control obtained from THP‐1 cells incubated for 6 h with 100 μ m DMF. Results are mean ± SEM of three independent experiments; * p <0.05 vs. control.

Article Snippet: Membranes were then incubated with antibodies directed against Nrf2 (H‐300, Santa Cruz biotechnology, Santa Cruz, USA, 1:1000 dilution in Tris buffer saline Tween 0.2 %, overnight incubation at 4 °C) or HO‐1 (Abcam, UK, 1:1000 dilution in 3 % bovine serum albumin solution prepared with Tris buffer saline‐Tween 0.2 %, 2 h incubation at room temperature).

Techniques: Western Blot, Positive Control, Incubation, Control

Journal: ChemistryOpen

Article Title: Effects of 3‐Bromo‐4,5‐dihydroisoxazole Derivatives on Nrf2 Activation and Heme Oxygenase‐1 Expression

doi: 10.1002/open.201800185

Figure Lengend Snippet: Electrophilic modulators of the Keap1‐Nrf2 pathway.

Article Snippet: Membranes were then incubated with antibodies directed against Nrf2 (H‐300, Santa Cruz biotechnology, Santa Cruz, USA, 1:1000 dilution in Tris buffer saline Tween 0.2 %, overnight incubation at 4 °C) or HO‐1 (Abcam, UK, 1:1000 dilution in 3 % bovine serum albumin solution prepared with Tris buffer saline‐Tween 0.2 %, 2 h incubation at room temperature).

Techniques: