Journal: Neurobiology of disease

Article Title: The BDNF Val66Met Variant Affects Gene Expression through miR-146b

doi: 10.1016/j.nbd.2015.03.004

Figure Lengend Snippet: Expression levels of candidate miR-146b targets and non-targets in the HPC of hBDNF Val/Met and hBDNF Met/Met mice, as compared to BDNF Val/Val mice

Article Snippet: All PCR primers and probes for coding genes were designed at Primer3 web site ( http://frodo.wi.mit.edu/ ) and purchased from Sigma Genosys (Sigma-Aldrich) and the sequences can be found in Supplementary Data Sheet 2 , except for Nptx2 (Mm00479438_m1, Applied Biosystems) and Clec16a (Mm00624340_m1, Applied Biosystems).

Techniques: Expressing, Binding Assay

Journal: World Journal of Psychiatry

Article Title: Serum neuronal pentraxin 2 is related to cognitive dysfunction and electroencephalogram slow wave/fast wave frequency ratio in epilepsy

doi: 10.5498/wjp.v13.i10.714

Figure Lengend Snippet: Serum levels of neuronal pentraxin 2 among study participants. Control: Healthy volunteers ( n = 30); Normal: Epilepsy patients with normal cognitive function ( n = 74); epilepsy patients with cognitive dysfunction ( n = 37). ECD: Epilepsy patients with cognitive dysfunction; NPTX2: Neuronal pentraxin 2.

Article Snippet: Serum NPTX2 was detected using a Human Neuronal pentraxin-2 (NPTX2) enzyme-linked immunosorbent assay kit (CSB-EL016030HU; CUSABIO, Houston, TX, United States).

Techniques: Control

Journal: World Journal of Psychiatry

Article Title: Serum neuronal pentraxin 2 is related to cognitive dysfunction and electroencephalogram slow wave/fast wave frequency ratio in epilepsy

doi: 10.5498/wjp.v13.i10.714

Figure Lengend Snippet: Correlation between Neuronal pentraxin 2 levels and Mini-mental state examination score, age and epilepsy duration. A to C: Correlation between serum levels of NPTX2 and MMSE score in (A), age (B), and epilepsy duration (C) in epilepsy patients with cognitive dysfunction. NPTX2: Neuronal pentraxin 2; MMSE: Mini-mental state examination.

Article Snippet: Serum NPTX2 was detected using a Human Neuronal pentraxin-2 (NPTX2) enzyme-linked immunosorbent assay kit (CSB-EL016030HU; CUSABIO, Houston, TX, United States).

Techniques:

Journal: World Journal of Psychiatry

Article Title: Serum neuronal pentraxin 2 is related to cognitive dysfunction and electroencephalogram slow wave/fast wave frequency ratio in epilepsy

doi: 10.5498/wjp.v13.i10.714

Figure Lengend Snippet: Comparison between neuronal pentraxin 2 serum levels and different variables in patients with epilepsy and cognitive dysfunction. A to E: Gender(A), education level (B), epilepsy type (C), epilepsy drug types (D), and treatment protocol (E). ECD: Epilepsy patients with cognitive dysfunction; NPTX2: Neuronal pentraxin 2; VPA: Valproate.

Article Snippet: Serum NPTX2 was detected using a Human Neuronal pentraxin-2 (NPTX2) enzyme-linked immunosorbent assay kit (CSB-EL016030HU; CUSABIO, Houston, TX, United States).

Techniques: Comparison

Journal: World Journal of Psychiatry

Article Title: Serum neuronal pentraxin 2 is related to cognitive dysfunction and electroencephalogram slow wave/fast wave frequency ratio in epilepsy

doi: 10.5498/wjp.v13.i10.714

Figure Lengend Snippet: Relationship between NPTX2 levels and ECG values in different regions. Correlation between NPTX2 serum levels and EEG values in the frontal (A), central (B), and temporal (C) regions of patients with epilepsy and cognitive dysfunction. ECG: Electrocardiogram; NPTX2: Neuronal pentraxin 2.

Article Snippet: Serum NPTX2 was detected using a Human Neuronal pentraxin-2 (NPTX2) enzyme-linked immunosorbent assay kit (CSB-EL016030HU; CUSABIO, Houston, TX, United States).

Techniques:

Journal: World Journal of Psychiatry

Article Title: Serum neuronal pentraxin 2 is related to cognitive dysfunction and electroencephalogram slow wave/fast wave frequency ratio in epilepsy

doi: 10.5498/wjp.v13.i10.714

Figure Lengend Snippet: Receiver operating characteristic curve for predicting serum level values and electroencephalogram values in temporal region in patients with epilepsy and cognitive dysfunction. ECG: Electrocardiogram; NPTX2: Neuronal pentraxin 2.

Article Snippet: Serum NPTX2 was detected using a Human Neuronal pentraxin-2 (NPTX2) enzyme-linked immunosorbent assay kit (CSB-EL016030HU; CUSABIO, Houston, TX, United States).

Techniques:

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: Schematic structure of the human NPTX2 structure. The mRNA of NPTX2 has 5 exons. The CGI is located around exon 1. “UCSC Genome Browser (Human GRCh 37/hg19)”. https://genome.ucsc.edu/cgi-bin/hgGateway?hgsid=599344653_C3wSlv7f4i4BPmj1dN500QJ2IlmW (accessed on 30 August 2018).

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques:

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: List of primer sets used for PCR.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: Gene Expression, Sequencing

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: Correlation between DNA methylation levels of the NPTX2 in both frozen and FFPE materials of nine tumors (Pearson’s correlation coefficient test: ρ = 0.692, p = 0.039).

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: DNA Methylation Assay

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: ( A ) DNA methylation rate of the NPTX2 gene in thymomas (n = 15) and paired normal thymic tissues (n = 15) ( Left ), and in TC (n = 9) and paired normal thymic tissues (n = 9) ( Right ) (the paired t -test). Samples from the same patient are linked with straight lines. ( B ) DNA methylation rate of the NPTX2 gene in TET according to the World Health Organization histological classification. The upper and lower ends of the whiskers show the upper and lower extremes, the upper and lower edges of the boxes show the upper (75th) and lower (25th) quartiles, and horizontal lines across each box show medians. Data were analyzed using the Kruskal–Wallis test and Steel–Dwass test for multiple comparisons. Asterisks indicate significant differences between groups by steel-Dwass. (** p < 0.01) ( C ) Receiver operating characteristic curve (ROC) was used for determining the accuracy of the methylation signature used for TC detection from all tumors using NPTX2 methylation levels.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: DNA Methylation Assay, Methylation

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: DNA methylation status of the NPTX2 gene according to the Masaoka–Koga staging system. The upper and lower ends of the whiskers show the upper and lower extremes, the upper and lower edges of the boxes show the upper (75th) and lower (25th) quartiles, and the horizontal lines across each box show the medians.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: DNA Methylation Assay

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: ( A ) The mRNA levels of the NPTX2 gene in thymomas and paired normal thymic tissues (n = 7, ( Left )), and in TC and paired normal thymic tissues (n = 7, ( Right )) (the Wilcoxon signed-rank test). Samples from the same patient are linked with straight lines. ( B ) NPTX2 mRNA expression in TET according to the WHO histological classification (A + AB + B1, B2, B3, and TC). The upper and lower ends of the whiskers show the upper and lower extremes, the upper and lower edges of the boxes show the upper (75th) and lower (25th) quartiles, and horizontal lines across each box show medians. Data were analyzed using the Kruskal–Wallis test and Steel–Dwass test. ( C ) Receiver operating characteristic curve (ROC) used to determine the accuracy of the methylation signature used for TC detection from all tumors in NPTX2 mRNA expression. ( D ) Correlation between the DNA methylation and mRNA expression of NPTX2 in 33 tumors and 12 normal thymus tissues. Data were analyzed via Spearman’s rank correlation.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: Expressing, Methylation, DNA Methylation Assay

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: The mRNA expression of the NPTX2 gene according to the Masaoka–Koga staging system. The upper and lower ends of the whiskers show the upper and lower extremes, the upper and lower edges of the boxes show the upper (75th) and lower (25th) quartiles, and the horizontal lines across each box show the medians.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: Expressing

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: ( A ) Representative images of the immunohistochemically detected NPTX2 protein in tumors. Scale bar, 100 µm. ( a ) Nerve cells in the brain were used as a positive control in IHC for the NPTX2 protein. The cytoplasmic staining of NPTX2 was observed in nerve cells. ( b ) No staining was noted in thymoma cells. ( c ) Tumor cells in TC were moderately stained. ( d ) Tumor cells in NECTT were strongly and diffusely stained. ( B ) Positive rate of NPTX2 protein expression in TET according to the WHO histological classification. The positive rate was 56% in thymoma, 67% in TC, and 100% in NETCC. Thymoma type A, B1, B2, and B3, TC; thymic carcinoma, NECTT; neuroendocrine tumors of the thymus ( C ) Relationship between the mRNA expression and immunoreactivity of NPTX2. We performed 39 TET for IHC. Both mRNA and IHC were measured in 31 tumors. In total, 18 (58%) out of 31 tumors showed positive reactivity.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: Positive Control, Staining, Expressing

Journal: Cancers

Article Title: Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors †

doi: 10.3390/cancers16020329

Figure Lengend Snippet: ( A ) Relapse-free survival curves of TET patients with higher and lower DNA methylation levels of NPTX2. The median value (23.8) for the frequency of the DNA methylation of NPTX2 was used to divide patients into the hypermethylated and hypomethylated groups. The dotted line shows the lower DNA methylation of NPTX2 (n = 32), while the solid line shows the higher DNA methylation of NPTX2 (n = 22). p -values were calculated using the Log-rank test. ( B ) Relapse-free survival curves of patients with TET with higher and lower levels of NPTX2 mRNA expression. The median value (0.479) of NPTX2 mRNA expression was used to divide patients into the high- and low-expression groups. The dotted line shows the lower expression of NPTX2 (n = 16), and the solid line shows the higher expression of NPTX2 (n = 25). p -values were calculated using the Log-rank test.

Article Snippet: The Human Protein Atlas revealed moderate to strong cytoplasmic positivity for NPTX2 in rare carcinoid and renal cancers, while all remaining cancer tissues were negative [ ].

Techniques: DNA Methylation Assay, Expressing