Journal: Cells

Article Title: 2,3-Bisphosphoglycerate Mutase (BPGM), a Metabolic Player Shaping Stress-Adaptive Transcriptional States in Clear Cell Renal Cell Carcinoma

doi: 10.3390/cells15070633

Figure Lengend Snippet: BPGM expression is elevated in human clear cell renal cell carcinoma. ( a ) Representative immunoblot showing BPGM protein levels in ccRCC tumor tissue of four representative patients (P1–4) and matched adjacent normal kidney samples. ( b ) Paired analysis of BPGM protein expression in matched control and tumor samples from individual patients ( n = 61 paired samples). Each data point represents one biological replicate (individual patient sample). ( c ) Violin plot depicting the distribution of BPGM expression levels in normal kidney tissue and ccRCC samples, illustrating increased expression and interindividual variability in tumor tissue. The dashed line indicates median and dotted lines the interquartile range. Statistical analysis was performed using a paired Student’s t -test. Exact p -values are indicated in the figure.

Article Snippet: BPGM protein was detected using a polyclonal rabbit anti-BPGM antibody (catalog #NBP1-86064, Novus Biologicals, Centennial, CO, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cells

Article Title: 2,3-Bisphosphoglycerate Mutase (BPGM), a Metabolic Player Shaping Stress-Adaptive Transcriptional States in Clear Cell Renal Cell Carcinoma

doi: 10.3390/cells15070633

Figure Lengend Snippet: ccRCC cells show limited sensitivity to epigenetic stress and elevated BPGM expression. ( a , b ) Crystal violet assays of ( a ) 2102EP and ( b ) A498 cells after 24 h treatment with increasing concentrations of Vorinostat (VS; 2–8 µM). ( a ) n = 10 per condition. ( b ) n = 10 (control, 2 µM, 8 µM) and n = 9 (3 µM, 4 µM; one outlier removed). ( c , d ) Basal BPGM protein expression in 2102EP and A498 cells shown as representative immunoblot ( c ) and quantification ( d ) ( n = 6 per condition). ( e ) BPGM mRNA expression after 24 h VS treatment in 2102EP and A498 cells. Expression is shown relative to control. n = 6 (2102EP); n = 5 (A498 control; one outlier removed) and n = 6 (A498 VS). Data are presented as box plots (median with interquartile range; whiskers indicate min–max). Each data point represents one biological replicate. Statistical analysis was performed using Brown–Forsythe ANOVA with Dunnett’s T3 multiple comparisons test ( a , b ), Welch’s t -test ( d ), and unpaired Student’s t -test (2102EP) or Welch’s t -test (A498) for ( e ). Details on test selection and normality assessment are provided in Methods. For clarity, only step-wise comparisons along the concentration series are displayed in panels ( a , b ). Complete multiple comparisons are provided in . Color code: white: 2102EP control; beige: A498 control; red: 2102EP + vorinostat; blue: A498 + vorinostat. Exact p -values are indicated in the figure.

Article Snippet: BPGM protein was detected using a polyclonal rabbit anti-BPGM antibody (catalog #NBP1-86064, Novus Biologicals, Centennial, CO, USA).

Techniques: Expressing, Control, Western Blot, Selection, Concentration Assay

Journal: Military Medical Research

Article Title: Endophilin A2 controls touch and mechanical allodynia via kinesin-mediated Piezo2 trafficking.

doi: 10.1186/s40779-024-00520-z

Figure Lengend Snippet: Fig. 9 Hypothetical model illustrating that endophilin A2 (EndoA2) interacts with Piezo2 and KIF5B to form a complex to regulate the membrane trafficking of Piezo2 in sensory neurons and thus contributes to mechanical hypersensitivity

Article Snippet: DRG neurons from L4–L6 of mice were cultured for 2 d. Following fixation, DRG coverslips were treated with primary antibodies against Piezo2 (rabbit, 1:200, Novus, USA, NBP1-78,624) and NF200 (mouse, 1:200, Sigma, USA, N0142) and the appropriate secondary antibodies.

Techniques: Membrane