Journal: Molecular medicine reports

Article Title: Plumbagin induces apoptosis in human osteosarcoma through ROS generation, endoplasmic reticulum stress and mitochondrial apoptosis pathway.

doi: 10.3892/mmr.2017.7222

Figure Lengend Snippet: Figure 1. Plumbagin decreases cell viability in osteosarcoma cells. (A) The structure of plumbagin. (B) The MG63, U2OS and HOS osteosarcoma cells and osteoblast cells hFOB 1.19 were treated with indicated concentrations of plumbagin for 1 and 2 day, and cell viability was assessed by MTT assay. (C) The cells were treated incubated with plumbagin for 10 days. The colony forming cells were stained with crystal violet and photographed. The quantitative data are presented in the right‑lower panel. (D) MG63 cells were incubated with indicated conditions of plumbagin for 24 h, and the nucleus morphology were assessed by DAPI staining. Magnification, x40. Results are expressed as the mean ± standard error of the mean. *P<0.05 vs. controls.

Article Snippet: The human osteosarcoma cell lines (MG-63, U2OS, HOS) and osteoblast cell line (hFOB 1.19) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Incubation, Staining