Journal:

Article Title: Compensatory Adaptation to the Loss of Biological Fitness Associated with Acquisition of Fusidic Acid Resistance in Staphylococcus aureus

doi: 10.1128/AAC.49.4.1426-1431.2005

Figure Lengend Snippet: Plasmids used in this work

Article Snippet: Antibiotics were used at the following final concentrations: ampicillin, 100 μg/ml; chloramphenicol, 10 μg/ml; erythromycin, 10 μg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Plasmid Relevant characteristic(s) a Source or reference pUC19 Cloning vector; Amp r New England Biolabs pSB1 pUC19 carrying fusA of S. aureus RN4220 3 pHPS9 E. coli-S. aureus shuttle and expression vector; Ery r Cm r 11 pSB2 pHPS9 carrying fusA of S. aureus RN4220 3 pSB2-P406L pSB2 with mutant fusA (P406L) 3 pSB2-H457Y pSB2 with mutant fusA (H457Y) 3 pSB2-A67T pSB2 with mutant fusA (A67T) This work pSB2-A67T/P406L pSB2 with mutant fusA (A67T, P406L) This work pSB2-S416F pSB2 with mutant fusA (S416F) This work pSB2-S416F/H457Y pSB2 with mutant fusA (S416F, H457Y) This work Open in a separate window a The mutant fusA alleles on the pSB2 derivatives encode EF-G with the amino acid exchanges given in parentheses.

Techniques: Clone Assay, Plasmid Preparation, Expressing, Mutagenesis

Journal:

Article Title: Prevalence of, Antibody Response to, and Immunity Induced by Haemophilus ducreyi Hemolysin

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: E. coli BL21(DE3) Host for protein expression Novagen, Madison, Wis. H. aphrophilus ATCC 33389 Arnold Smith H. ducreyi 35000-TcA Strain 35000 with Tn 916 inserted in hhdB , nonhemolytic 50 H. ducreyi 35000-KmA Strain 35000 with Tn 1545-Δ3 inserted in hhdB , nonhemolytic 50 H. ducreyi 35000ΔAPC Strain 35000 with cat cassette in hhdA gene, nonhemolytic 54 H. haemoglobinophilus ATCC 19416 ATCC, Manassas, Va. H. haemolyticus ATCC 33390 Arnold Smith H. influenzae ATCC 33911 Arnold Smith H. influenzae Rd Marilyn Roberts H. parainfluenzae ATCC 33392 Arnold Smith H. paraphrophilus ATCC 29237 Arnold Smith H. segmis ATCC 33393 Arnold Smith T. equigenitalis ATCC 35865 Arnold Smith Plasmids pUC19 E. coli cloning vector, Ap r GibcoBRL pCR2.1 TA cloning vector for cloning PCR products, Amp r Kan r Invitrogen, San Diego, Calif. pET24+ Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, no translation initiation signals, Kan r Novagen pET24a+ Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, Kan r Novagen pBCKS Cm r plasmid derived from pUC19 with KS multiple cloning site Stratagene, La Jolla, Calif. pPT384-ETa hhdBA genes cloned into pET24a+ Sst I- Sal I This study pLSSK Shuttle vector with ori , sulA , and strA genes from pLS88 and multiple cloning sites and lacZ gene from pBluescript SK 54 pPT384 hhdBA gene region in pTZ18 in same orientation as lac promoter 50 pPT376BCKS 5.8-kb Bgl II fragment containing part of hhdB and all of hhdA cloned into pBCKS Sst I- Sal I 50 pETBABN hhdBA genes cloned into pET24+ Bam HI- Sal I This study pLSBAHis+ hhdBA genes cloned into pLSSK in same orientation as lac promoter.

Techniques: Plasmid Preparation, Cloning, Expressing, TA Cloning, Sequencing, Derivative Assay, Clone Assay

Journal: Cell reports

Article Title: Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs

doi: 10.1016/j.celrep.2024.113777

Figure Lengend Snippet:

Article Snippet: Plasmids for mammalian cell expression of Cas12a were gifted from various labs (Zhang, Kleinstiver, Li, and Ekker)., , , , The plasmid encoding crRNAs under U6 promoter was obtained from Joung and Kleinstiver labs (Addgene #114087) and subsequently modified at the scaffold region to create the nine distinct crRNAs via site-directed mutagenesis.

Techniques: Virus, Recombinant, Protease Inhibitor, DNA Extraction, Mutagenesis, Nucleic Acid Purification, Cloning, Plasmid Preparation, Software