Journal: Methods and Protocols

Article Title: Novel In Vivo Mouse Cryoablation Model to Explore Unique Therapeutic Approaches for Premalignant Columnar Lesions

doi: 10.3390/mps4010006

Figure Lengend Snippet: Preliminary results using the cryoablation procedure. ( A ) The experimental set-up of the validation of the cryoablation procedure is according to with minor adjustments. ( B ) Fluorescent image of untreated K5-GFP mice stomach. ( C ) Fluorescent image of vehicle-treated K5-GFP mice, 14 days post-cryoablation. ( D ) Fluorescent image of K5-GFP mice, 14 days post-cryoablation, treated with mNoggin (orally, 1× day). The delimitation of the original SCJ that separated the squamous and the columnar epithelium before the ablation is indicated by the dotted white line. i.p.: intraperitoneal injection; PO: Per os. , oral administration.

Article Snippet: Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA, USA; cat. no.: 10010023); Formalin solution, neutral buffered, 10% (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: HT501128-4L); Ethanol (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: 51976-500ML-F); Xylene (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: 108298); Hematoxylin solution according to Mayer (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: 51275); Eosin Y solution 1% (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: 1170811000); DPX Mountant (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: 06522-100ML); Tissue-Plus TM O.C.T. compound (Fisher Scientific, Waltham, MA, USA; cat. no.: 23-730-571); DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) (Thermo Fisher Scientific, Waltham, MA, USA; cat. no.: 62247); Recombinant mouse noggin protein (R&D Systems, McKinley, MN, USA; cat. no.: 1967-NG); Tamoxifen (Sigma-Aldrich, St. Louis, MO, USA; cat. no.: T5648).

Techniques: Biomarker Discovery, Injection