nitrocefin Search Results


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Biosynth Carbosynth nitrocefin addition
Figure 3. Cellular activity of AIP analogues. A) Activation dose–response curves for AIP-I (^) and analogues hb-Asp AIP-I (*), D5A hb-Ala AIP-I (~), and D5A b-Ala AIP-I (*). AIPs were added in increasing concentrations to an exponential phase culture of a group I S. aureus reporter strain (RN9222). Samples were removed after 60 min and assayed for b-lactamase activity by the <t>nitrocefin</t> method. Data are plotted as percent maximal activation, nor- malized for cell density, versus peptide concentration. Errors; SEM, n=3. B) Inhibition dose–response curves for D5A AIP (*) and analogues D5A hb- Ala AIP-I (~), D5A b-Ala AIP-I (*), Hcy AIP-I (^), Hcy AIP-III (&), and hb-Asp AIP-I (!). Group II reporter cells (RN9367) were treated with a fixed concen- tration of AIP-II (125 nm) and varying concentrations of the indicated ana- logues. b-lactamase activity was measured, and the normalized data are pre- sented as in panel A. The dose–response data for the D5A b-Ala AIP-I ana- logue do not fit an inhibition curve. Errors; SEM, n=3. Additional cellular activity data is provided in Figures S3–S5.
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Toronto Research Chemicals nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
Nitrocefin, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson nitrocefin becton dickinson biosciences
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
Nitrocefin Becton Dickinson Biosciences, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson dryslide nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Becton Dickinson nitrocefin (ncf)
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
Nitrocefin (Ncf), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson nitrocefin-based test cefinase
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
Nitrocefin Based Test Cefinase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Unipath Limited nitrocefin (δε 482 = +17,500 m −1 cm −1 )
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
Nitrocefin (δε 482 = +17,500 M −1 Cm −1 ), supplied by Unipath Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson nitrocefin disk test cefinase
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Becton Dickinson bbl™ dryslide™ nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Unipath Limited nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Unipath Limited chromogenic cephalosporin nitrocefin
Effect of Gracilaria sp. extracts on VarG. Using <t>nitrocefin</t> as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.
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Image Search Results


Figure 3. Cellular activity of AIP analogues. A) Activation dose–response curves for AIP-I (^) and analogues hb-Asp AIP-I (*), D5A hb-Ala AIP-I (~), and D5A b-Ala AIP-I (*). AIPs were added in increasing concentrations to an exponential phase culture of a group I S. aureus reporter strain (RN9222). Samples were removed after 60 min and assayed for b-lactamase activity by the nitrocefin method. Data are plotted as percent maximal activation, nor- malized for cell density, versus peptide concentration. Errors; SEM, n=3. B) Inhibition dose–response curves for D5A AIP (*) and analogues D5A hb- Ala AIP-I (~), D5A b-Ala AIP-I (*), Hcy AIP-I (^), Hcy AIP-III (&), and hb-Asp AIP-I (!). Group II reporter cells (RN9367) were treated with a fixed concen- tration of AIP-II (125 nm) and varying concentrations of the indicated ana- logues. b-lactamase activity was measured, and the normalized data are pre- sented as in panel A. The dose–response data for the D5A b-Ala AIP-I ana- logue do not fit an inhibition curve. Errors; SEM, n=3. Additional cellular activity data is provided in Figures S3–S5.

Journal: Chembiochem : a European journal of chemical biology

Article Title: Increasing AIP Macrocycle Size Reveals Key Features of agr Activation in Staphylococcus aureus.

doi: 10.1002/cbic.201500006

Figure Lengend Snippet: Figure 3. Cellular activity of AIP analogues. A) Activation dose–response curves for AIP-I (^) and analogues hb-Asp AIP-I (*), D5A hb-Ala AIP-I (~), and D5A b-Ala AIP-I (*). AIPs were added in increasing concentrations to an exponential phase culture of a group I S. aureus reporter strain (RN9222). Samples were removed after 60 min and assayed for b-lactamase activity by the nitrocefin method. Data are plotted as percent maximal activation, nor- malized for cell density, versus peptide concentration. Errors; SEM, n=3. B) Inhibition dose–response curves for D5A AIP (*) and analogues D5A hb- Ala AIP-I (~), D5A b-Ala AIP-I (*), Hcy AIP-I (^), Hcy AIP-III (&), and hb-Asp AIP-I (!). Group II reporter cells (RN9367) were treated with a fixed concen- tration of AIP-II (125 nm) and varying concentrations of the indicated ana- logues. b-lactamase activity was measured, and the normalized data are pre- sented as in panel A. The dose–response data for the D5A b-Ala AIP-I ana- logue do not fit an inhibition curve. Errors; SEM, n=3. Additional cellular activity data is provided in Figures S3–S5.

Article Snippet: Immediately following Nitrocefin addition, b-lactam hydrolysis was monitored by OD490 readings taken every 45 s over 20 min. All peptide stock solution concentrations were determined by amino acid analysis (AAA) carried out by New England Peptide, Inc. (Gardner, MA).

Techniques: Activity Assay, Analogues, Activation Assay, Concentration Assay, Inhibition

Effect of Gracilaria sp. extracts on VarG. Using nitrocefin as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.

Journal: ACS Omega

Article Title: Characterization and Potentiating Effects of the Ethanolic Extracts of the Red Seaweed Gracillaria sp. on the Activity of Carbenicillin against Vibrios

doi: 10.1021/acsomega.2c05288

Figure Lengend Snippet: Effect of Gracilaria sp. extracts on VarG. Using nitrocefin as a substrate, and the variety of OD486 was measured. EDTA was used as positive control with the concentration of 1 mM. GE was used with the concentration of 1 μg/mL. All treatments were performed in triplicate and expressed as mean ± SD ( n = 3). Significantly different between different groups and control are showed with *.

Article Snippet: Nitrocefin was obtained from Toronto Research Chemicals, Canada.

Techniques: Positive Control, Concentration Assay, Control