Journal: European Journal of Endocrinology

Article Title: Monocarboxylate transporter 8 deficiency: altered thyroid morphology and persistent high triiodothyronine/thyroxine ratio after thyroidectomy

doi: 10.1530/eje-11-0369

Figure Lengend Snippet: Figure 3 Histological and morphometric alterations in thyroids from Mct8-deficient mice. (A) QPCR analyses in the murine thyroid gland. Mct8 is the most abundantly expressed T3 transporter. Seven out of 14 transporters tested are shown. Liver-specific Slc10a1 was used as a negative control. Inset: Mct8 protein expression in thyroid compared with brain. Transferrin receptor (TfR) served as membrane protein control. (B) Immunohistochemistry for Mct8 in the murine thyroid. The protein is located in the basolateral membrane of thyrocytes. Thyroid tissue from an Mct8K/y mouse (right) served as negative control. Follicles are indicated by broken lines. Scale bar 50 mm. (C) Follicular cell tumour with papillary structures and nuclear features of papillary carcinoma (nuclear infoldings, grooves and other irregularities, elongation and overlapping) in Mct8-deficient thyroids as analysed by H&E staining, immunohistochemistry for thyroglobulin (Tg) and sodium/iodide symporter (NIS). Note the basolateral localisation of NIS in the aberrant epithelium. Scale bars 50 mm. (D) Follicular size is increased in Mct8-deficient thyroids. Cross-sectional follicular areas were measured, grouped according to size and the fraction of follicles falling into each group was plotted against the area. (E) Follicular epithelial extension is significantly increased in Mct8-deficient thyroids. ***P!0.001, Student’s t-test, two-sided, unpaired. (F) Activity of type I deiodinase is unaltered in Mct8-deficient thyroids. nZ6 animals/group.

Article Snippet: Immunohistochemical stainings were performed as described (11) with antibodies against Mct8 (1:250, Atlas Antibodies), thyroglobulin (Tg; 1:750, Thermo Fisher Scientific, Waltham, MD, USA) and sodium/iodide symporter (NIS; 1:1200, Acris Antibodies, Herford, Germany).

Techniques: Negative Control, Expressing, Membrane, Control, Immunohistochemistry, Staining, Activity Assay

Journal: PLoS ONE

Article Title: Resveratrol Inhibits Sodium/Iodide Symporter Gene Expression and Function in Rat Thyroid Cells

doi: 10.1371/journal.pone.0107936

Figure Lengend Snippet: Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

Article Snippet: After this transfer, the membranes were incubated according to the manufacturer instructions, using a mouse monoclonal anti-NIS antibody (NBP1-70342, Novus Biologicals Europe, Cambridge, UK).

Techniques: Control, Immunofluorescence, Staining, Negative Control, Microscopy