nicd1 Search Results


pt3 ef1a nicd1  (Addgene inc)
93
Addgene inc pt3 ef1a nicd1
Pt3 Ef1a Nicd1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pt3 ef1a nicd1/product/Addgene inc
Average 93 stars, based on 1 article reviews
pt3 ef1a nicd1 - by Bioz Stars, 2026-03
93/100 stars
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pt3 ef1ah nicd1  (Addgene inc)
93
Addgene inc pt3 ef1ah nicd1
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
Pt3 Ef1ah Nicd1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pt3 ef1ah nicd1/product/Addgene inc
Average 93 stars, based on 1 article reviews
pt3 ef1ah nicd1 - by Bioz Stars, 2026-03
93/100 stars
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xin chen  (Addgene inc)
91
Addgene inc xin chen
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
Xin Chen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xin chen/product/Addgene inc
Average 91 stars, based on 1 article reviews
xin chen - by Bioz Stars, 2026-03
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expression plasmids for nicd1 and mst1  (General Biosystems Inc)
90
General Biosystems Inc expression plasmids for nicd1 and mst1
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
Expression Plasmids For Nicd1 And Mst1, supplied by General Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression plasmids for nicd1 and mst1/product/General Biosystems Inc
Average 90 stars, based on 1 article reviews
expression plasmids for nicd1 and mst1 - by Bioz Stars, 2026-03
90/100 stars
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r26nicd1 strain  (Jackson Laboratory)
90
Jackson Laboratory r26nicd1 strain
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
R26nicd1 Strain, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r26nicd1 strain/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
r26nicd1 strain - by Bioz Stars, 2026-03
90/100 stars
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mini-ph meter nicd-1  (METTLER TOLEDO)
90
METTLER TOLEDO mini-ph meter nicd-1
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
Mini Ph Meter Nicd 1, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mini-ph meter nicd-1/product/METTLER TOLEDO
Average 90 stars, based on 1 article reviews
mini-ph meter nicd-1 - by Bioz Stars, 2026-03
90/100 stars
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gfp–nicd1 plasmid  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare gfp–nicd1 plasmid
(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. <t>n-NICD1</t> <t>(nuclear-NICD1),</t> n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.
Gfp–Nicd1 Plasmid, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp–nicd1 plasmid/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
gfp–nicd1 plasmid - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. n-NICD1 (nuclear-NICD1), n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.

Journal: bioRxiv

Article Title: Growth hormone induces mitotic catastrophe of podocytes and contributes to proteinuria

doi: 10.1101/597344

Figure Lengend Snippet: (A) qRT-PCR analysis showing the expression of Notch1 and Jag1 in HPC treated with or without conditioned medium (CM) of 50% from CTL and 50% GH treated podocytes for 48h. Mean±SD. (n=6). ****p<0.0001 by student t-test. mRNA levels were normalized to β-Actin and presented as fold-change on the y-axis. (B) Immunoblotting analysis from HPC treated with or without CM of 50% from CTL and 50% GH treated podocytes for 48hr. (n=3). (C) Immunoblotting analysis from HPC treated with or without GH (250 and 500ng/ml), TGFβ-1 (5ng/ml), GH (500ng/ml) + SB431542 (100nM/ml) and GH (500ng/ml) + AG490 (10μM/ml) for 48hr. (n=3). SB431542 is an inhibitor for TGFBR1 and AG490 is an inhibitor for GHR. n-NICD1 (nuclear-NICD1), n-HES1 (nuclear HES1) and n-HEY1(nuclear HEY1). (D) HPC cells transfected with specific siRNA targeting TGFBR1 or scramble RNA (Scr-RNA) were subjected to immunoblotting. (n=3). (E) Immunofluorescence for nuclear co-localization of NICD1 (red color), HES1 (purple color) and counterstained with DAPI (blue color) in HPC from CTL vs treatments for 48hr. Magnification x630. Scale bar=2Oμm. (n=3). (F) HES1 reporter activity was measured in HPC from CTL vs treatment for 48h. Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. DAPT (5μg/ml). (G) qRT-PCR analysis of Notch1 and JAG1 expression in MPC from CTL or GH (1.5mg/kg bw), GH+ SB431542 (1mg/kg bw) and GH+ AG490 (1mg/kg bw) administered mice (each group, n=6). Mean±SD. (n=6). ****p<0.0001 by Student’s t-test. Each dot represents the average value of three independent experiments from a single mouse. (H) Immunoblotting analysis for MPC from CTL vs treatment group of mice. (n=3). β-Actin and Lamin-B1 served as an internal control.

Article Snippet: The transient transfection of pT3-EF1aH NICD1; an overexpressing vector from Addgene (#86500) and its empty parental vector, pT3-EF1aH to the podocytes using Xfect polymer (DSS Takara Bio, New Delhi, India) as per manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Transfection, Immunofluorescence, Activity Assay

(A&B) Immunoblotting analysis for indicated genes expression in HPC (48hr treatment) and MPC from CTL vs treatment group. (n=3). (C) Immunoblotting analysis from HPC under ectopic expression of NICD1 (NICD1-OE). Empty vector denotes negative control. (n=3). (D) Immunofluo-rescence for the RhoA (green color) and counterstained with DAPI (blue color) in HPC from CTL vs treatment for 48hr. Magnification x630. Scale bar=20μm. (n=3). White arrowhead indicates the dislocalization of RhoA from midbody. (E-H) Immunoblotting analysis from HPC and MPC from CTL vs treatment group. (n=3). (I) HPC from CTL vs treatment for 48hr, stained with FITC AnnexinV and PI, and analyzed by flow cytometry. (n=4). The values of the representative histograms indicate the percentage of podocytes, in the lower left quadrant (Live cells), lower right quadrant (early apoptosis), upper right quadrant (late apoptosis) and upper left quadrant (necrotic cells). (J) Representative TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining by DAB in glomerular sections from CTL vs treatment mice group. Magnification x630, Scale bar=20μm.(n=3). β-Actin served as internal control.

Journal: bioRxiv

Article Title: Growth hormone induces mitotic catastrophe of podocytes and contributes to proteinuria

doi: 10.1101/597344

Figure Lengend Snippet: (A&B) Immunoblotting analysis for indicated genes expression in HPC (48hr treatment) and MPC from CTL vs treatment group. (n=3). (C) Immunoblotting analysis from HPC under ectopic expression of NICD1 (NICD1-OE). Empty vector denotes negative control. (n=3). (D) Immunofluo-rescence for the RhoA (green color) and counterstained with DAPI (blue color) in HPC from CTL vs treatment for 48hr. Magnification x630. Scale bar=20μm. (n=3). White arrowhead indicates the dislocalization of RhoA from midbody. (E-H) Immunoblotting analysis from HPC and MPC from CTL vs treatment group. (n=3). (I) HPC from CTL vs treatment for 48hr, stained with FITC AnnexinV and PI, and analyzed by flow cytometry. (n=4). The values of the representative histograms indicate the percentage of podocytes, in the lower left quadrant (Live cells), lower right quadrant (early apoptosis), upper right quadrant (late apoptosis) and upper left quadrant (necrotic cells). (J) Representative TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining by DAB in glomerular sections from CTL vs treatment mice group. Magnification x630, Scale bar=20μm.(n=3). β-Actin served as internal control.

Article Snippet: The transient transfection of pT3-EF1aH NICD1; an overexpressing vector from Addgene (#86500) and its empty parental vector, pT3-EF1aH to the podocytes using Xfect polymer (DSS Takara Bio, New Delhi, India) as per manufacturer’s instructions.

Techniques: Western Blot, Expressing, Plasmid Preparation, Negative Control, Staining, Flow Cytometry, TUNEL Assay

(A) Representative images of immunohistochemical staining for TGFβ-1 and NICD1 by DAB in the glomerulus sections from healthy (n=8) and DN group (n=14). Magnification x630. Scale bar=20μm. (n=3). (B&C) Representative image of H&E stain in glomerular sections from healthy and DN group. Black arrowhead indicates bi-nucleated and detached podocyte. Magnification x630. Scale bar=20μm. Zoomed picture emphasizes a bi-nucleated and detached podocyte. (D) Representative image of MT stain in glomerular sections from healthy and DN group. Magnification x630. Scale bar=20μm. (E) Immunoblotting analysis for TGF-β1 in the urine samples from healthy (n=4) and DN group (n=9). (F) Quantification of TGF-β1 in the urine samples from healthy (n=8) and DN (n=14). Mean±SD. **p<0.001 by student’s t-test. (G) Urine samples from healthy (n=4) and DN (n=9) were resolved on SDS-PAGE and stained with Coomassie Blue. BSA= Bovine serum albumin. M=protein marker. (H) Nephroseq ( www.nephroseq.org ) analysis comparing HES1, MIKI67, PCNA, RHOA, TGFBR1, and TP53 expression levels in non-diabetic (n=13) versus diabetic nephropathy (n=9) (Woroniecka Diabetes glom, nephroseq.org). Data indicate that the expression of these genes increased >1.5-fold in the DN. (I) Schematic illustration of GH action on podocyte cell cycle entry and apoptosis via TGF-β1 mediated Notch1 activation.

Journal: bioRxiv

Article Title: Growth hormone induces mitotic catastrophe of podocytes and contributes to proteinuria

doi: 10.1101/597344

Figure Lengend Snippet: (A) Representative images of immunohistochemical staining for TGFβ-1 and NICD1 by DAB in the glomerulus sections from healthy (n=8) and DN group (n=14). Magnification x630. Scale bar=20μm. (n=3). (B&C) Representative image of H&E stain in glomerular sections from healthy and DN group. Black arrowhead indicates bi-nucleated and detached podocyte. Magnification x630. Scale bar=20μm. Zoomed picture emphasizes a bi-nucleated and detached podocyte. (D) Representative image of MT stain in glomerular sections from healthy and DN group. Magnification x630. Scale bar=20μm. (E) Immunoblotting analysis for TGF-β1 in the urine samples from healthy (n=4) and DN group (n=9). (F) Quantification of TGF-β1 in the urine samples from healthy (n=8) and DN (n=14). Mean±SD. **p<0.001 by student’s t-test. (G) Urine samples from healthy (n=4) and DN (n=9) were resolved on SDS-PAGE and stained with Coomassie Blue. BSA= Bovine serum albumin. M=protein marker. (H) Nephroseq ( www.nephroseq.org ) analysis comparing HES1, MIKI67, PCNA, RHOA, TGFBR1, and TP53 expression levels in non-diabetic (n=13) versus diabetic nephropathy (n=9) (Woroniecka Diabetes glom, nephroseq.org). Data indicate that the expression of these genes increased >1.5-fold in the DN. (I) Schematic illustration of GH action on podocyte cell cycle entry and apoptosis via TGF-β1 mediated Notch1 activation.

Article Snippet: The transient transfection of pT3-EF1aH NICD1; an overexpressing vector from Addgene (#86500) and its empty parental vector, pT3-EF1aH to the podocytes using Xfect polymer (DSS Takara Bio, New Delhi, India) as per manufacturer’s instructions.

Techniques: Immunohistochemical staining, Staining, Western Blot, SDS Page, Marker, Expressing, Activation Assay