Journal: Journal of Extracellular Vesicles

Article Title: Bioorthogonally surface‐edited extracellular vesicles based on metabolic glycoengineering for CD44‐mediated targeting of inflammatory diseases

doi: 10.1002/jev2.12077

Figure Lengend Snippet: In vivo biodistribution of PHA‐EVs in inflammatory animal models with tumour and RA. (a) In vivo fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in CIA mice. (b) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the inflamed joints of CIA mice as a function of time ( n = 3). (c) Distribution of CD44 and EV in the inflamed synovium of CIA mice. (d) Whole‐body fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in PC3 tumour‐bearing mice. (e) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the tumour site as a function of time ( n = 3). (f) Distribution of CD44 and EV in tumour tissue. (b and e) * P < 0.05, analyzed by two‐way ANOVA. Error bars represent standard error

Article Snippet: Cy5.5 NHS ester dye was purchased from GE Healthcare (IL, USA).

Techniques: In Vivo, Fluorescence

Journal: Nano letters

Article Title: Energy Transfer from Perovskite Nanocrystals to Dye Molecules Does Not Occur by FRET.

doi: 10.1021/acs.nanolett.9b03779

Figure Lengend Snippet: Figure 3: Polarization anisotropy of the PL of single NCs and Cy3 molecules excited by

Article Snippet: Samples with immobilized NC/dye pairs were prepared by spin coating a ~100 nm thick film of PMMA from a solution of PMMA, NCs and either Cy3 (Cyanin 3 NHS-ester from Lumiprobe, article number 11020) or Cy5 (Cyanin 5 NHS-ester from Lumiprobe, article number 13020) in toluene at nanomolar concentrations onto a transparent substrate.

Techniques:

Journal: Nano letters

Article Title: Energy Transfer from Perovskite Nanocrystals to Dye Molecules Does Not Occur by FRET.

doi: 10.1021/acs.nanolett.9b03779

Figure Lengend Snippet: Figure 4. Spectra and spectral dynamics of single NCs and single EET-pumped Cy3

Article Snippet: Samples with immobilized NC/dye pairs were prepared by spin coating a ~100 nm thick film of PMMA from a solution of PMMA, NCs and either Cy3 (Cyanin 3 NHS-ester from Lumiprobe, article number 11020) or Cy5 (Cyanin 5 NHS-ester from Lumiprobe, article number 13020) in toluene at nanomolar concentrations onto a transparent substrate.

Techniques:

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Biotin receptor-mediated intracellular delivery of synthetic polypeptide-protein complexes.

doi: 10.1016/j.jconrel.2023.03.051

Figure Lengend Snippet: Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein (Cy5-lyso zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: To monitor cellular internalization of the protein cargo, lysozyme used in this H. Li et al. Journal of Controlled Release 357 (2023) 333–341 experiment was fluorescently labelled using sulfo-Cyanine 5 (Cy5) succinimidyl (NHS) ester (Lumiprobe), according to the manufacturer’s protocol (as described in Supporting information, Methods).

Techniques: Confocal Microscopy, Incubation, Staining