Journal: Scientific reports

Article Title: Fragment size and dynamics of EGFR-mutated tumor-derived DNA provide prognostic information regarding EGFR-TKI efficacy in patients with EGFR-mutated NSCLC.

doi: 10.1038/s41598-022-17848-y

Figure Lengend Snippet: Figure 2. (A) A scatterplot graph and correlation analysis (r = 0.837) results for VAF of the EGFR Ex.19 deletion or L858R mutations (X axis) and the EGFR T790M mutations (Y axis) (B) The cis–trans configuration between the activating and resistance mutations was analyzed by target RNA-Seq analysis of tissue biopsy specimens. This figure shows an example of a cis pattern of Ex.19 deletion and T790M visualized by the IGV genome browser. The region bridging Ex.19 and Ex.20 of EGFR was amplified by RT-PCR and the amplicon was sequenced by NGS. Most sequencing reads with a 15-bp Ex.19 deletion (represented by the blue line and number) harbor T790M mutations on the same RNA molecule. (C) Comparison of the VAF of EGFR T790M at point A between the no disappearance, A → B disappearance, and A → C disappearance groups. NS; no significance. (D) Kaplan–Meier plot of PFS for osimertinib treatment. Comparison of PFS between the no disappearance, A → B disappearance, and A → C disappearance groups (p = 0.442).

Article Snippet: Sequence libraries for the Illumina platform from these PCR products were constructed using the GenNext® NGS Library Prep Kit (Toyobo).

Techniques: RNA Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, Sequencing, Comparison

Journal: Animal Microbiome

Article Title: Bacterial topography of the upper and lower respiratory tract in pigs

doi: 10.1186/s42523-023-00226-y

Figure Lengend Snippet: Relative abundance (%) of the most abundant genera identified in nostrils and trachea by shotgun and 16S rRNA sequencing

Article Snippet: Illumina adapters were added to the partial 16S rRNA gene-specific amplicons, which were further processed using the Quick-16S NGS Library Prep Kit (Zymo Research).

Techniques:

Journal: PLOS Global Public Health

Article Title: qPCR in a suitcase for rapid Plasmodium falciparum and Plasmodium vivax surveillance in Ethiopia

doi: 10.1371/journal.pgph.0000454

Figure Lengend Snippet: Equipment and consumables required for qPCR screening. One extraction kit lasts for 8 x 96-well plates. The number and weight of all consumables and reagents reflects quantities required for 8 x 96 samples.

Article Snippet: DNA storage plate , ThermoFisher AB 0765 , 8 , 670 , .

Techniques: Extraction, Blocking Assay, Transferring, DNA Extraction, Stripping Membranes