Journal: Retrovirology

Article Title: HTLV-1 Tax mutants that do not induce G 1 arrest are disabled in activating the anaphase promoting complex

doi: 10.1186/1742-4690-4-35

Figure Lengend Snippet: Immunoblot analyses of HeLa cells transduced with wild-type or mutant tax alleles . HeLa cells transduced with the wild-type or the respective mutant tax allele were harvested. Cell lysates were prepared, resolved by SDS-12% PAGE, and probed with antibodies against Tax, cyclin B1, I-κBα, NF-κB, p52, p27 KIP1 , p21 CIP1 / WAF1 , Skp2, and actin, respectively.

Article Snippet: Total cell proteins were then resolved by SDS/12% PAGE, transferred to nitrocellulose membrane and probed with antibodies (Santa Cruz Biotechnology) against cyclin B1 (sc-752), actin (sc-1616), I-κBα (sc-1643), p52-NFκB2 (sc-7386), Skp2 (sc-7164), p21 CIP1 / WAF1 (sc-397), and p27 KIP 1 (sc-1641).

Techniques: Western Blot, Transduction, Mutagenesis

Journal: Journal of Cell Communication and Signaling

Article Title: Long non-coding RNA DIO3OS/let-7d/NF-κB2 axis regulates cells proliferation and metastasis of thyroid cancer cells

doi: 10.1007/s12079-020-00589-w

Figure Lengend Snippet: Effects of DIO3OS on thyroid cancer cells. a DIO3OS knockdown was generated in BCPAP and TPC-1 cells by the transfection of si1-DIO3OS or si2-DIO3OS, as confirmed by real-time PCR. Next, BCPAP and TPC-1 cells were transfected with si2-DIO3OS and examined for b cell viability by CCK-8 assay; c DNA synthesis capacity by EdU assay; d, e invasion capacity by Transwell assay and migration capacity by Wound healing assay; f the protein levels of ki-67 and PCNA by Immunoblotting. N = 3, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: NF-κB2 knockdown in cells was generated by the transfection of si1-NF-κB2 and si2- NF-κB2 (GenePharma). let-7d overexpression or inhibition in cells was generated by the transfection of let-7d mimics or let-7d inhibitor (GenePharma, Shanghai, China).

Techniques: Knockdown, Generated, Transfection, Real-time Polymerase Chain Reaction, CCK-8 Assay, DNA Synthesis, EdU Assay, Transwell Assay, Migration, Wound Healing Assay, Western Blot

Journal: Journal of Cell Communication and Signaling

Article Title: Long non-coding RNA DIO3OS/let-7d/NF-κB2 axis regulates cells proliferation and metastasis of thyroid cancer cells

doi: 10.1007/s12079-020-00589-w

Figure Lengend Snippet: Expression and function of NF-κB2 in thyroid cancer. a mRNAs that are co-expressed with DIO3OS were analyzed and selected based on online TCGA data; selected mRNAs were applied for KEGG signaling annotation analysis and these mRNAs were enriched in hsa04514-Cell adhesion molecules (CAMs) and Cytokine-cytokine receptor interaction. Key factors of these signaling pathways, ICAM-1 and VCAM1, were significantly correlated with DIO3OS. b Transcription factor enrichment analysis was performed to screen for transcription factors related to DIO3OS, ICAM-1, and VCAM1. NF-κB2 was selected. c The protein content and distribution of NF-κB2 in thyroid cancer and normal tissues were examined by IHC staining. d BCPAP and TPC-1 cells were transfected with si1-DIO3OS or si2-DIO3OS and examined for the protein levels of NF-κB2 by Immunoblotting. e NF-κB2 knockdown was generated in BCPAP and TPC-1 cells by the transfection of si1-NF-κB2 or si2-NF-κB2, as confirmed by real-time PCR. Next, BCPAP and TPC-1 cells were transfected with si-NF-κB2 or si2-NF-κB2 and examined for f cell viability by CCK-8 assay; g DNA synthesis capacity by EdU assay; h, i invasion capacity by Transwell assay and migration capacity by Wound healing assay; j the protein levels of ki-67, PCNA, ICAM-1, and VCAM by Immunoblotting. N = 3, *P < 0.05, **P < 0.01

Article Snippet: NF-κB2 knockdown in cells was generated by the transfection of si1-NF-κB2 and si2- NF-κB2 (GenePharma). let-7d overexpression or inhibition in cells was generated by the transfection of let-7d mimics or let-7d inhibitor (GenePharma, Shanghai, China).

Techniques: Expressing, Protein-Protein interactions, Immunohistochemistry, Transfection, Western Blot, Knockdown, Generated, Real-time Polymerase Chain Reaction, CCK-8 Assay, DNA Synthesis, EdU Assay, Transwell Assay, Migration, Wound Healing Assay