Journal: Pharmaceutical Biology

Article Title: Methamphetamine leads to the alterations of microRNA profiles in the nucleus accumbens of rats

doi: 10.1080/13880209.2020.1803366

Figure Lengend Snippet: The effects of METH on neuron differentiation by immunofluorescence. The NSE positive cells decreased (A) while GFAP positive cells (B) increased in the striatum, hippocampus, and NAc after METH treatment.

Article Snippet: The primary antibody of NSE (neuron specific enolase, catalog No. AF5169) and GFAP (glial fibrillary acidic protein, catalog No. AF2594) was obtained from the R&D system (Minneapolis, MN, USA).

Techniques: Immunofluorescence

Journal: Cell Reports Methods

Article Title: Methods for culturing adult CNS neurons reveal a CNS conditioning effect

doi: 10.1016/j.crmeth.2022.100255

Figure Lengend Snippet:

Article Snippet: Biotin-Antibody Cocktail , Miltenyi , 130-115-390.

Techniques: Control, Recombinant, Sterility, Flow Cytometry, Isolation, Software

Journal: PLOS ONE

Article Title: Short communication: Upregulation of hypoxia/reoxygenation-induced Shc3 by downregulated miR-455-5p, suppresses trophoblast invasion and is associated with placental inflammation and angiogenesis in preeclampsia

doi: 10.1371/journal.pone.0314544

Figure Lengend Snippet: (A) Heatmap of RNA-Seqs. (B) Selection of potential miR-455-5p downstream genes. (C) Schematic diagram of luciferase reporter-Shc3 3’UTR constructs. (D) The outcomes of miR-455-5p overexpression on the luciferase activity in HTR8/SVneo cells transfected with the WT or MUT luciferase reporter constructs. (E) The effect of miR-455-5p inhibition and overexpression on Shc3 mRNA expression in HTR8/SVneo cells. (F) The level of Shc3 protein expression after miR-455-5p inhibition and overexpression in HTR8/SVneo. Data presented shows the average values ± standard deviation (**p < 0.01, *p < 0.05). The findings presented were obtained from a minimum of three independent experiments.

Article Snippet: SHC3, Beta Actin antibodies and HRP-conjugated Affinipure Goat Anti Rabbit IgG(H+L) were obtained from Proteintech (Wuhan, China).

Techniques: Selection, Luciferase, Construct, Over Expression, Activity Assay, Transfection, Inhibition, Expressing, Standard Deviation

Journal: PLOS ONE

Article Title: Short communication: Upregulation of hypoxia/reoxygenation-induced Shc3 by downregulated miR-455-5p, suppresses trophoblast invasion and is associated with placental inflammation and angiogenesis in preeclampsia

doi: 10.1371/journal.pone.0314544

Figure Lengend Snippet: (A) The migration and invasion of HTR8/SVneo cells with miR-455-5p overexpression plasmids co-cultured with the plasmids of Shc3, or Shc3-NC in H/R. (B) Flow cytometry data of the apoptosis of HTR8/SVneo cells with miR-455-5p overexpression plasmids co-cultured with the plasmids expressing Shc3 or Shc3-NC in H/R. (C) Western blot results of Shc3 expression in HTR8/SVneo cells transfected with the miR-455-5p mimic, Shc3, or Shc3-NC. (D) The results of Shc3 expression examined by immunohistochemistry. Data presented indicate the average values ± standard deviation (**p < 0.01, *p < 0.05). The findings presented were obtained from a minimum of three independent experiments.

Article Snippet: SHC3, Beta Actin antibodies and HRP-conjugated Affinipure Goat Anti Rabbit IgG(H+L) were obtained from Proteintech (Wuhan, China).

Techniques: Migration, Over Expression, Cell Culture, Flow Cytometry, Expressing, Western Blot, Transfection, Immunohistochemistry, Standard Deviation

Journal: PLOS ONE

Article Title: Short communication: Upregulation of hypoxia/reoxygenation-induced Shc3 by downregulated miR-455-5p, suppresses trophoblast invasion and is associated with placental inflammation and angiogenesis in preeclampsia

doi: 10.1371/journal.pone.0314544

Figure Lengend Snippet: (A~B). H/R promotes the production of Shc3, which may be transported to the extracellular space through medium/large extracellular vesicles. (A) Western blot results of Shc3 expression in hypoxia, H/R, and normoxia, respectively. (B) Volcano plot of Shc3 mRNA expression in placental tissue, extracellular medium/large vesicles and small vesicles. (C~E). Shc3 is involved in placental inflammation and angiogenesis inhibition. (C) Hematoxylin-eosin staining results on normotensive placental tissues and high Shc3 expression PE placental tissues. (D) The level of Shc3 in EA.hy926 after transfection with the plasmids of Shc3, and Shc3-NC, in H/R compared with controls. (E) Angiogenesis of EA.hy926 after transfection with the plasmids of Shc3, and Shc3-NC in H/R, as determined by the number of master junction and master segment length.

Article Snippet: SHC3, Beta Actin antibodies and HRP-conjugated Affinipure Goat Anti Rabbit IgG(H+L) were obtained from Proteintech (Wuhan, China).

Techniques: Western Blot, Expressing, Inhibition, Staining, Transfection