Journal: Frontiers in Neuroanatomy

Article Title: Neuronize: a tool for building realistic neuronal cell morphologies

doi: 10.3389/fnana.2013.00015

Figure Lengend Snippet: (A) Confocal microscopy image of an intracellularly injected layer III pyramidal neuron of the human cingulate cortex. (B) High magnification image showing basal dendrites and spines (inset). (C) Computer-aided tracing in 3D (Neurolucida) of the neuron shown in (A) . (D) Same image illustrated in (B) showing a superimposed 3D tracing of the positions of the dendritic spines. (E) Digital representation of the pyramidal neuron shown in (A) and (C) . Left: the neuron definition where each row represents a morphological point (identifier, type of point, (X,Y,Z) coordinates, radius at that point, and parent identifier). Right: depiction of the morphological skeleton defined on the left. Scale bar in (D) : 50 μm in (A) , 10 μm in ( B, D ) (4.5 μm inset).

Article Snippet: Data points of neuron morphology were extracted using Neurolucida Confocal package (MicroBrightField; Figures ).

Techniques: Confocal Microscopy, Injection

Journal: Frontiers in Neuroanatomy

Article Title: Neuronize: a tool for building realistic neuronal cell morphologies

doi: 10.3389/fnana.2013.00015

Figure Lengend Snippet: Comparison of the same neuron using different 3D representations . Models generated using NeuroConstruct (A,D) , Neurolucida (B,E) and our proposed method (C,F) . Note the unconnected elements and the absence of a realistic soma in (A,B). (D–F) Close-up view of the same bifurcation with the three different technologies.

Article Snippet: Data points of neuron morphology were extracted using Neurolucida Confocal package (MicroBrightField; Figures ).

Techniques: Comparison, Generated