neurod1 Search Results


gene exp neurod1 mm01946604 s1  (Thermo Fisher)
85
Thermo Fisher gene exp neurod1 mm01946604 s1
Gene Exp Neurod1 Mm01946604 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/us12583928-592-44--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp neurod1 mm01946604 s1 - by Bioz Stars, 2026-07
85/100 stars
  Buy from Supplier
af2746  (R&D Systems)
93
R&D Systems af2746
Af2746, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pm37323565-141-28-25?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
af2746 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
2010 addgene  (Addgene inc)
90
Addgene inc 2010 addgene
2010 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pm30638745-231-84-85?v=Addgene+inc
Average 90 stars, based on 1 article reviews
2010 addgene - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
rabbit polyclonal anti neurod1  (Proteintech)
93
Proteintech rabbit polyclonal anti neurod1
Rabbit Polyclonal Anti Neurod1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pmc11615295__41467_2024_54927_MOESM4_ESM-46-0-4?v=Proteintech
Average 93 stars, based on 1 article reviews
rabbit polyclonal anti neurod1 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
neurod1  (Rockland Immunochemicals)
90
Rockland Immunochemicals neurod1
Neurod1, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pmc02275424-175-22-35?v=Rockland+Immunochemicals
Average 90 stars, based on 1 article reviews
neurod1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
goat anti neurod1  (R&D Systems)
99
R&D Systems goat anti neurod1
Goat Anti Neurod1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/bio_rxiv__730150-148-48-50?v=R%26D+Systems
Average 99 stars, based on 1 article reviews
goat anti neurod1 - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
human neurod1 shrna  (OriGene)
91
OriGene human neurod1 shrna
Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes <t>(NEUROD1,</t> HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.
Human Neurod1 Shrna, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pm36563694-309-11-18?v=OriGene
Average 91 stars, based on 1 article reviews
human neurod1 shrna - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
ef1a neurod1 p2a hygro barcode  (Addgene inc)
94
Addgene inc ef1a neurod1 p2a hygro barcode
Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes <t>(NEUROD1,</t> HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.
Ef1a Neurod1 P2a Hygro Barcode, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pmc08515093__mmc6-133-9-10?v=Addgene+inc
Average 94 stars, based on 1 article reviews
ef1a neurod1 p2a hygro barcode - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
shrna ctrl plasmids origene cat  (OriGene)
91
OriGene shrna ctrl plasmids origene cat
Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes <t>(NEUROD1,</t> HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.
Shrna Ctrl Plasmids Origene Cat, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pm36563694-160-27-30?v=OriGene
Average 91 stars, based on 1 article reviews
shrna ctrl plasmids origene cat - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
neurod1  (Addgene inc)
90
Addgene inc neurod1
Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes <t>(NEUROD1,</t> HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.
Neurod1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pm23773995-239-6-13?v=Addgene+inc
Average 90 stars, based on 1 article reviews
neurod1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
neurod1  (Novus Biologicals)
90
Novus Biologicals neurod1
Histological and immunohistochemical examination of pancreatic tissue in patients with congenital hyperinsulinism and in controls. Hematoxylin and eosin staining, insulin, <t>NeuroD1</t> and Nkx2.2, Isl1, chromogranin A, somatostatin and DR1, DR2, DR5, SSTR2 and SSTR5; x50. Note: HEO – hematoxylin and eosin, Somat – somatostatin, ChrA – chromogranin A.
Neurod1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/pmc08419459-120-81-89?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
neurod1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
nd1  (Novus Biologicals)
92
Novus Biologicals nd1
Figure 4. Western blot analysis of mitochondrial proteins. A, 5 g of total mitochondrial proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with antibodies specific for <t>ND1,</t> ND4, ND5, ND6, CO1, CYTB, and ATP6 and with VDAC as a loading control, respectively. B, quantification of mitochondrial proteins. The levels of mitochondrial proteins in mutant and control cell lines were determined as described elsewhere (17, 32). The average of three determinations for each cell line is shown. Graph details and symbols are explained in the legend to Fig. 2.
Nd1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurod1/10__1074_slash_jbc__ra119__010598-240-11-31?v=Novus+Biologicals
Average 92 stars, based on 1 article reviews
nd1 - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier

Image Search Results


Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes (NEUROD1, HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.

Journal: Cell stem cell

Article Title: An insulin hypersecretion phenotype precedes pancreatic β cell failure in MODY3 patient-specific cells.

doi: 10.1016/j.stem.2022.12.001

Figure Lengend Snippet: Figure 4. Membrane potential-related genes are deregulated in MODY3 b cells (A) Schematic of the purification of CPEP+/NKX6-1+ S7 b cells (green) from 1-corA and 1-mut lines by MARIS (method for analyzing RNA following intracellular sorting)29 prior to bulk RNA sequencing. n = 4 independent experiments. (B) RT-qPCR of CPEP+/NKX6-1+ S7 cells assessing MODY genes (NEUROD1, HNF4A, PAX4, and RFX6), ion channels (KCNA3, KCNH6, KCNK9, HCN1, ATP2A3, and CACNA1B), membrane potential regulators (SRC and ANK2), and the KATP subunits (ABCC8 and KCNJ11). n = 7 (1-corB) or 12 (1-corA and 1-mut) independent experiments. Data are represented as mean + SD. Wilcoxon matched-pairs signed-rank test analysis. *, **, and *** represent statistical significance at p % 0.05, p % 0.01, and p % 0.001, respectively. (C) Overrepresented GO terms related to ion transport and membrane potential regulation within the RNA sequencing dataset. (D) Heatmap of differentially expressed genes from the GO term ‘‘regulation of membrane potential.’’ The S7 cells were generated in four independent exper- iments (1–4). See also Figures S4 and S5.

Article Snippet: Then the HEK 293T cells were transfected with 5 mg of human NEUROD1 shRNA or shRNA Ctrl plasmids (OriGene, TL311201), 6 mg of packaging plasmids (OriGene, TR30037) in 1.5 mL of Opti-MEM (Life Technologies, 31985-070), and 33 mL of TurboFectin Transfection Reagent (OriGene, TF81001) based on the manufacturer’s recommendations.

Techniques: Membrane, RNA Sequencing, Quantitative RT-PCR, Generated

Histological and immunohistochemical examination of pancreatic tissue in patients with congenital hyperinsulinism and in controls. Hematoxylin and eosin staining, insulin, NeuroD1 and Nkx2.2, Isl1, chromogranin A, somatostatin and DR1, DR2, DR5, SSTR2 and SSTR5; x50. Note: HEO – hematoxylin and eosin, Somat – somatostatin, ChrA – chromogranin A.

Journal: Frontiers in Endocrinology

Article Title: Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children

doi: 10.3389/fendo.2021.710947

Figure Lengend Snippet: Histological and immunohistochemical examination of pancreatic tissue in patients with congenital hyperinsulinism and in controls. Hematoxylin and eosin staining, insulin, NeuroD1 and Nkx2.2, Isl1, chromogranin A, somatostatin and DR1, DR2, DR5, SSTR2 and SSTR5; x50. Note: HEO – hematoxylin and eosin, Somat – somatostatin, ChrA – chromogranin A.

Article Snippet: Immunohistochemical analysis of pancreatic samples from 35 patients with CHI and 10 patients from the control group was carried out according to a standard method ( ) using antibodies to: chromogranin A (rabbit polyclonal antibody, dilution 1:400, Diagnostic BioSystems, The Netherlands); insulin (mouse monoclonal antibody, clone K36aC10, 1:50 dilution, Diagnostic BioSystems, The Netherlands); Isl1 (rabbit polyclonal antibody, 1:1000 dilution, ThermoFisher, USA); Nkx 2.2 (rabbit monoclonal antibody, clone EPR14638, dilution 1:20, Abcam, UK); somatostatin (SST, rabbit polyclonal antibody, dilution 1:1000, Dako, Denmark); NeuroD1 (mouse monoclonal antibody, clone 3H8, dilution 1:1000, Novus Biologicals, USA); somatostatin receptor type 2 (SSTR2, rabbit monoclonal antibody, clone EP149, dilution 1:200, Abcam, UK); somatostatin receptor type 5 (SSTR5, rabbit monoclonal antibody, clone UMB4, dilution 1:100, Abcam, UK); type 1 dopamine receptor (DR1, rabbit polyclonal antibody, dilution 1:50, Novus Biologicals, USA); type 2 dopamine receptor (DR2, mouse monoclonal antibody, clone B10, dilution 1:100, Santa Cruz Biotechnology, USA); and type 5 dopamine receptor (DR5, rabbit polyclonal antibody, 1:100 dilution, ThermoFisher, USA).

Techniques: Immunohistochemical staining, Staining

Pancreas of a patient with a focal form of congenital hyperinsulinism. (A) – NeuroD1, (B) – insulin; x50.

Journal: Frontiers in Endocrinology

Article Title: Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children

doi: 10.3389/fendo.2021.710947

Figure Lengend Snippet: Pancreas of a patient with a focal form of congenital hyperinsulinism. (A) – NeuroD1, (B) – insulin; x50.

Article Snippet: Immunohistochemical analysis of pancreatic samples from 35 patients with CHI and 10 patients from the control group was carried out according to a standard method ( ) using antibodies to: chromogranin A (rabbit polyclonal antibody, dilution 1:400, Diagnostic BioSystems, The Netherlands); insulin (mouse monoclonal antibody, clone K36aC10, 1:50 dilution, Diagnostic BioSystems, The Netherlands); Isl1 (rabbit polyclonal antibody, 1:1000 dilution, ThermoFisher, USA); Nkx 2.2 (rabbit monoclonal antibody, clone EPR14638, dilution 1:20, Abcam, UK); somatostatin (SST, rabbit polyclonal antibody, dilution 1:1000, Dako, Denmark); NeuroD1 (mouse monoclonal antibody, clone 3H8, dilution 1:1000, Novus Biologicals, USA); somatostatin receptor type 2 (SSTR2, rabbit monoclonal antibody, clone EP149, dilution 1:200, Abcam, UK); somatostatin receptor type 5 (SSTR5, rabbit monoclonal antibody, clone UMB4, dilution 1:100, Abcam, UK); type 1 dopamine receptor (DR1, rabbit polyclonal antibody, dilution 1:50, Novus Biologicals, USA); type 2 dopamine receptor (DR2, mouse monoclonal antibody, clone B10, dilution 1:100, Santa Cruz Biotechnology, USA); and type 5 dopamine receptor (DR5, rabbit polyclonal antibody, 1:100 dilution, ThermoFisher, USA).

Techniques:

The proportion of exocrinocytes expressing NeuroD1 in the field of view in various forms of congenital hyperinsulinism. Data are presented as M ± SD. (*) - the difference compared to the control is statistically significant at p <0.01.

Journal: Frontiers in Endocrinology

Article Title: Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children

doi: 10.3389/fendo.2021.710947

Figure Lengend Snippet: The proportion of exocrinocytes expressing NeuroD1 in the field of view in various forms of congenital hyperinsulinism. Data are presented as M ± SD. (*) - the difference compared to the control is statistically significant at p <0.01.

Article Snippet: Immunohistochemical analysis of pancreatic samples from 35 patients with CHI and 10 patients from the control group was carried out according to a standard method ( ) using antibodies to: chromogranin A (rabbit polyclonal antibody, dilution 1:400, Diagnostic BioSystems, The Netherlands); insulin (mouse monoclonal antibody, clone K36aC10, 1:50 dilution, Diagnostic BioSystems, The Netherlands); Isl1 (rabbit polyclonal antibody, 1:1000 dilution, ThermoFisher, USA); Nkx 2.2 (rabbit monoclonal antibody, clone EPR14638, dilution 1:20, Abcam, UK); somatostatin (SST, rabbit polyclonal antibody, dilution 1:1000, Dako, Denmark); NeuroD1 (mouse monoclonal antibody, clone 3H8, dilution 1:1000, Novus Biologicals, USA); somatostatin receptor type 2 (SSTR2, rabbit monoclonal antibody, clone EP149, dilution 1:200, Abcam, UK); somatostatin receptor type 5 (SSTR5, rabbit monoclonal antibody, clone UMB4, dilution 1:100, Abcam, UK); type 1 dopamine receptor (DR1, rabbit polyclonal antibody, dilution 1:50, Novus Biologicals, USA); type 2 dopamine receptor (DR2, mouse monoclonal antibody, clone B10, dilution 1:100, Santa Cruz Biotechnology, USA); and type 5 dopamine receptor (DR5, rabbit polyclonal antibody, 1:100 dilution, ThermoFisher, USA).

Techniques: Expressing, Control

Confocal laser scanning microscopy of the pancreas in congenital hyperinsulinism. (A–D) focal form, (E, F) diffuse form. (A, F) blue fluorescence of cell nuclei (DAPI). (B, F) green fluorescence of NeuroD1 in nuclei. (C, G) red fluorescence of ChrA in cytoplasm. (D, H) greenish-blue/turquoise fluorescence of NeuroD1 and red fluorescence of ChrA in the same cells; x400.

Journal: Frontiers in Endocrinology

Article Title: Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children

doi: 10.3389/fendo.2021.710947

Figure Lengend Snippet: Confocal laser scanning microscopy of the pancreas in congenital hyperinsulinism. (A–D) focal form, (E, F) diffuse form. (A, F) blue fluorescence of cell nuclei (DAPI). (B, F) green fluorescence of NeuroD1 in nuclei. (C, G) red fluorescence of ChrA in cytoplasm. (D, H) greenish-blue/turquoise fluorescence of NeuroD1 and red fluorescence of ChrA in the same cells; x400.

Article Snippet: Immunohistochemical analysis of pancreatic samples from 35 patients with CHI and 10 patients from the control group was carried out according to a standard method ( ) using antibodies to: chromogranin A (rabbit polyclonal antibody, dilution 1:400, Diagnostic BioSystems, The Netherlands); insulin (mouse monoclonal antibody, clone K36aC10, 1:50 dilution, Diagnostic BioSystems, The Netherlands); Isl1 (rabbit polyclonal antibody, 1:1000 dilution, ThermoFisher, USA); Nkx 2.2 (rabbit monoclonal antibody, clone EPR14638, dilution 1:20, Abcam, UK); somatostatin (SST, rabbit polyclonal antibody, dilution 1:1000, Dako, Denmark); NeuroD1 (mouse monoclonal antibody, clone 3H8, dilution 1:1000, Novus Biologicals, USA); somatostatin receptor type 2 (SSTR2, rabbit monoclonal antibody, clone EP149, dilution 1:200, Abcam, UK); somatostatin receptor type 5 (SSTR5, rabbit monoclonal antibody, clone UMB4, dilution 1:100, Abcam, UK); type 1 dopamine receptor (DR1, rabbit polyclonal antibody, dilution 1:50, Novus Biologicals, USA); type 2 dopamine receptor (DR2, mouse monoclonal antibody, clone B10, dilution 1:100, Santa Cruz Biotechnology, USA); and type 5 dopamine receptor (DR5, rabbit polyclonal antibody, 1:100 dilution, ThermoFisher, USA).

Techniques: Confocal Laser Scanning Microscopy, Fluorescence

Confocal laser scanning microscopy of the pancreas in congenital hyperinsulinism. (A–D) focal form, (E, F) diffuse form. (A, F) blue fluorescence of cell nuclei (DAPI). (B, F) green fluorescence of NeuroD1 in nuclei. (C, G) red fluorescence of DR2 in cytoplasm. (D, H) greenish-blue/turquoise fluorescence of NeuroD1 and red fluorescence of DR2 in the same cells; x400.

Journal: Frontiers in Endocrinology

Article Title: Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children

doi: 10.3389/fendo.2021.710947

Figure Lengend Snippet: Confocal laser scanning microscopy of the pancreas in congenital hyperinsulinism. (A–D) focal form, (E, F) diffuse form. (A, F) blue fluorescence of cell nuclei (DAPI). (B, F) green fluorescence of NeuroD1 in nuclei. (C, G) red fluorescence of DR2 in cytoplasm. (D, H) greenish-blue/turquoise fluorescence of NeuroD1 and red fluorescence of DR2 in the same cells; x400.

Article Snippet: Immunohistochemical analysis of pancreatic samples from 35 patients with CHI and 10 patients from the control group was carried out according to a standard method ( ) using antibodies to: chromogranin A (rabbit polyclonal antibody, dilution 1:400, Diagnostic BioSystems, The Netherlands); insulin (mouse monoclonal antibody, clone K36aC10, 1:50 dilution, Diagnostic BioSystems, The Netherlands); Isl1 (rabbit polyclonal antibody, 1:1000 dilution, ThermoFisher, USA); Nkx 2.2 (rabbit monoclonal antibody, clone EPR14638, dilution 1:20, Abcam, UK); somatostatin (SST, rabbit polyclonal antibody, dilution 1:1000, Dako, Denmark); NeuroD1 (mouse monoclonal antibody, clone 3H8, dilution 1:1000, Novus Biologicals, USA); somatostatin receptor type 2 (SSTR2, rabbit monoclonal antibody, clone EP149, dilution 1:200, Abcam, UK); somatostatin receptor type 5 (SSTR5, rabbit monoclonal antibody, clone UMB4, dilution 1:100, Abcam, UK); type 1 dopamine receptor (DR1, rabbit polyclonal antibody, dilution 1:50, Novus Biologicals, USA); type 2 dopamine receptor (DR2, mouse monoclonal antibody, clone B10, dilution 1:100, Santa Cruz Biotechnology, USA); and type 5 dopamine receptor (DR5, rabbit polyclonal antibody, 1:100 dilution, ThermoFisher, USA).

Techniques: Confocal Laser Scanning Microscopy, Fluorescence

Figure 4. Western blot analysis of mitochondrial proteins. A, 5 g of total mitochondrial proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with antibodies specific for ND1, ND4, ND5, ND6, CO1, CYTB, and ATP6 and with VDAC as a loading control, respectively. B, quantification of mitochondrial proteins. The levels of mitochondrial proteins in mutant and control cell lines were determined as described elsewhere (17, 32). The average of three determinations for each cell line is shown. Graph details and symbols are explained in the legend to Fig. 2.

Journal: Journal of Biological Chemistry

Article Title: Contribution of a mitochondrial tyrosyl-tRNA synthetase mutation to the phenotypic expression of the deafness-associated tRNASer(UCN) 7511A>G mutation

doi: 10.1074/jbc.ra119.010598

Figure Lengend Snippet: Figure 4. Western blot analysis of mitochondrial proteins. A, 5 g of total mitochondrial proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with antibodies specific for ND1, ND4, ND5, ND6, CO1, CYTB, and ATP6 and with VDAC as a loading control, respectively. B, quantification of mitochondrial proteins. The levels of mitochondrial proteins in mutant and control cell lines were determined as described elsewhere (17, 32). The average of three determinations for each cell line is shown. Graph details and symbols are explained in the legend to Fig. 2.

Article Snippet: The antibodies used for this investigation were from Abcam (GAPDH (ab8245), ND1 (ab74257), ND5 (ab92624), ND6 (ab81212), CO1 (ab14705), ATP6 (ab101908), NDUFS3 (ab14711), and total OXPHOS human WB antibody mixture (ab110411)), Novus (ND4 (NBP2-47365)), and Proteintech (VDAC (10866-1-AP), CYTB (55090-1-AP), and COX10 (10611-2-AP)).

Techniques: Western Blot, Control, Mutagenesis