negative cd43 isolation kit Search Results


easysep human b cell enrichment kit w/o cd43 depletion  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc easysep human b cell enrichment kit w/o cd43 depletion
Easysep Human B Cell Enrichment Kit W/O Cd43 Depletion, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative cd43 isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec negative cd43 isolation kit

Negative Cd43 Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative selection for cd43  (Miltenyi Biotec)
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Miltenyi Biotec negative selection for cd43

Negative Selection For Cd43, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spn 9001 histostaintm sp kit  (OriGene)
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OriGene spn 9001 histostaintm sp kit

Spn 9001 Histostaintm Sp Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse b cell isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec mouse b cell isolation kit
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Mouse B Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin antibody  (Miltenyi Biotec)
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Miltenyi Biotec biotin antibody
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Biotin Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd43 microbeads b cell enrichment kit  (Becton Dickinson)
90
Becton Dickinson anti-cd43 microbeads b cell enrichment kit
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Anti Cd43 Microbeads B Cell Enrichment Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v (annexin v pe apoptosis detection kit  (Becton Dickinson)
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Becton Dickinson annexin v (annexin v pe apoptosis detection kit
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Annexin V (Annexin V Pe Apoptosis Detection Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd43 microbeads kit  (Miltenyi Biotec)
93
Miltenyi Biotec cd43 microbeads kit
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Cd43 Microbeads Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monarch dna gel extraction kit neb cat  (New England Biolabs)
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New England Biolabs monarch dna gel extraction kit neb cat
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Monarch Dna Gel Extraction Kit Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd43 b cell isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec cd43 b cell isolation kit
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
Cd43 B Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b cell isolation kit ii  (Miltenyi Biotec)
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Miltenyi Biotec b cell isolation kit ii
Tet2 deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of <t>PBs</t> <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.
B Cell Isolation Kit Ii, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell Reports

Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells

doi: 10.1016/j.celrep.2019.10.018

Figure Lengend Snippet:

Article Snippet: Negative CD43- Isolation Kit , Miltenyi Biotec , Cat# 130-049-801.

Techniques: Western Blot, Recombinant, Methylation, Adjuvant, Enzyme-linked Immunosorbent Assay, Isolation, cDNA Synthesis, SYBR Green Assay, DNA Library Preparation, Purification, Bicinchoninic Acid Protein Assay, Microarray, Ex Vivo, Generated, Software, Red Blood Cell Lysis, Staining, Lysis

Tet2 deficiency impairs memory B‐cell and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.

Journal: HemaSphere

Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis

doi: 10.1002/hem3.70296

Figure Lengend Snippet: Tet2 deficiency impairs memory B‐cell and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.

Article Snippet: In vitro ‐iGCB, iPC, and induced memory B cells (iMBC) were generated and defined according to established protocols and nomenclature., Splenic murine naïve B‐cells were isolated from 2‐ to 3‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) according to the manufacturer's instructions (yield >90% CD19+ B220+).

Techniques: Clinical Proteomics, Cell Differentiation, Cytometry, Expressing, Two Tailed Test