nec495a001mc Search Results


90
Revvity c 2dg
(A) Western blots of long bones from 8-week-old Prx1-Cre Notch2fl/fl (PN2) mice and Notch2fl/fl (N2) littermates. Each lane is for a separate mouse. (B) Glucose consumption and lactate production in N2 and PN2 BMSCs with or without Jagged1 treatment for 48 hours. n = 3. (C–I) Analyses of 7-week-old N2 or PN2 mice that received 3PO or DMSO for 4 weeks starting at 3 weeks of age, including body weight (C), blood glucose (D), <t>14C-2DG</t> uptake in bone (E), μCT images (F), μCT quantification (G), and dynamic histomorphometry (H) of trabecular bone in the proximal tibia, as well as serum CTX-I levels (I). n = 3. *P < 0.05, by 2-way ANOVA followed by Bonferroni’s post hoc test (C–E, H, and I) or 2-tailed Student’s t test (B and G). BV, bone volume; DPM, disintegrations per minute; TV, total volume.
C 2dg, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nec495a001mc/Deoxy-D-glucose%2C+2-%5B1-14C%5D-%2C+Aqueous+Sol%2C+Specific+Activity%3A+45-60+mCi+(1%2E66-2%2E22+GBq)%2Fmmol%2C%3E97%25/pmc06264656-452-13-14
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(A) Western blots of long bones from 8-week-old Prx1-Cre Notch2fl/fl (PN2) mice and Notch2fl/fl (N2) littermates. Each lane is for a separate mouse. (B) Glucose consumption and lactate production in N2 and PN2 BMSCs with or without Jagged1 treatment for 48 hours. n = 3. (C–I) Analyses of 7-week-old N2 or PN2 mice that received 3PO or DMSO for 4 weeks starting at 3 weeks of age, including body weight (C), blood glucose (D), 14C-2DG uptake in bone (E), μCT images (F), μCT quantification (G), and dynamic histomorphometry (H) of trabecular bone in the proximal tibia, as well as serum CTX-I levels (I). n = 3. *P < 0.05, by 2-way ANOVA followed by Bonferroni’s post hoc test (C–E, H, and I) or 2-tailed Student’s t test (B and G). BV, bone volume; DPM, disintegrations per minute; TV, total volume.

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Figure Lengend Snippet: (A) Western blots of long bones from 8-week-old Prx1-Cre Notch2fl/fl (PN2) mice and Notch2fl/fl (N2) littermates. Each lane is for a separate mouse. (B) Glucose consumption and lactate production in N2 and PN2 BMSCs with or without Jagged1 treatment for 48 hours. n = 3. (C–I) Analyses of 7-week-old N2 or PN2 mice that received 3PO or DMSO for 4 weeks starting at 3 weeks of age, including body weight (C), blood glucose (D), 14C-2DG uptake in bone (E), μCT images (F), μCT quantification (G), and dynamic histomorphometry (H) of trabecular bone in the proximal tibia, as well as serum CTX-I levels (I). n = 3. *P < 0.05, by 2-way ANOVA followed by Bonferroni’s post hoc test (C–E, H, and I) or 2-tailed Student’s t test (B and G). BV, bone volume; DPM, disintegrations per minute; TV, total volume.

Article Snippet: The mice were rested for 30 minutes before being injected i.p. with 14 C-2DG (PerkinElmer, NEC495A001MC) at 10 μCi per 20 g body weight and then sacrificed after 2 hours for collection of tibiae and femurs.

Techniques: Western Blot