Journal: Nature

Article Title: Control of mitochondrial function and cell growth by the atypical cadherin Fat1.

doi: 10.1038/nature20170

Figure Lengend Snippet: Figure 1 | Fat1 fragments localize to SMC mitochondria and interact with inner mitochondrial membrane proteins. a, Analysis of 30 top-ranked TAP–MS-validated Fat1 ICD interactors. IMM and OMM, inner and outer mitochondrial membrane, respectively (see Extended Data Table 1). b, Mitochondrial cluster in bioinformatic analysis of TAP–MS data (see Extended Data Fig. 1a). c, Fractionation of mouse aortic SMCs, followed by SDS–PAGE and immunoblotting. C, cytoplasmic; Mit, mitochondrial; Ms, microsomal; WL, whole-cell lysate. Arrowhead, full-length Fat1; bracket, Fat1 ICD species; red asterisk, mitochondrial-specific Fat1 ICD fragments; blue asterisk, non-specific signal. d, Mouse SMC confocal imaging. Scale bar, 10 μm. e, Co-immunoprecipitation of Fat1 ICD and NDUFS3 in 293T cells. For gel source data, see Supplementary Fig. 1.

Article Snippet: The following antibodies were from Santa Cruz: complex I NDUFS3 (1:600 for WB, sc374282), c-Myc (1:20,000 for WB, sc789), β -catenin (1:800 for WB, sc7963), PGC1-α (1:150 for WB, sc13067), PGC1-β (1:100 for WB, sc373771), Tfam (1:150 for WB, sc23588), Mfn1 (1:600 for WB, sc50330), Mfn2 (1:600 for WB, sc50331), Acta2 (1:200 for IF, sc32351), nitrotyrosine (1:250 for IF, sc32757), GAPDH (1:8,000 for WB, sc25778), anti-rabbit IgG HRP (1:10,000, sc2030), and anti-goat IgG HRP (1:10,000, sc2056).

Techniques: Membrane, Fractionation, SDS Page, Western Blot, Imaging, Immunoprecipitation

Journal: Metabolism Open

Article Title: Supplementation with aspalathin and sulforaphane protects cultured cardiac cells against dyslipidemia-associated oxidative damage

doi: 10.1016/j.metop.2025.100346

Figure Lengend Snippet: The list of TaqMan probes used in the study.

Article Snippet: NADH: Ubiquinone Oxidoreductase Core Subunit S3 , Ndufs3 , Rn01484390_m1.

Techniques: Gene Assay

Journal: Metabolism Open

Article Title: Supplementation with aspalathin and sulforaphane protects cultured cardiac cells against dyslipidemia-associated oxidative damage

doi: 10.1016/j.metop.2025.100346

Figure Lengend Snippet: Aspalathin and sulforaphane increased the mRNA expression of some markers involved in mitochondrial function in cardiomyoblasts exposed to palmitic acid. Briefly, H9c2 cardiomyoblasts were pretreated with 1 μM aspalathin (Asp) and 10 μM sulforaphane (Sul) as well as 2.5 μM Simvastatin (Simva) which was used as a comparative control. Thereafter, cells were co-treated with 0.25 mM palmitic acid (Pal) for an additional 24 h. The mRNA expression levels of peroxisome proliferator-activated receptor gamma coactivator 1-alpha ( Pprgc1α ), nuclear respiratory factor 1 ( Nrf1 ), NADH: ubiquinone oxidoreductase core subunit S3 ( Ndufs3 ), ubiquinol-cytochrome C reductase complex assembly factor 1 ( Uqcc1 ), and uncoupling protein 2 ( Ucp2 ) were quantified ( A, B, C, D, and E , respectively). Results are presented as the mean ± standard error of the mean (SEM) from three independent experiments, each with three technical repeats (n = 3), relative to the experimental control (Ctrl). Comparisons between groups were performed using student’s t -test (and nonparametric test). Statistical significance was represented by ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared to the experimental control; and # p < 0.05 compared to the palmitic acid control.

Article Snippet: NADH: Ubiquinone Oxidoreductase Core Subunit S3 , Ndufs3 , Rn01484390_m1.

Techniques: Expressing, Control

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Divergent skeletal muscle mitochondrial phenotype between male and female patients with chronic heart failure

doi: 10.1002/jcsm.12488

Figure Lengend Snippet: Relative mRNA expression levels of several key mitochondrial gene transcripts. Compared with controls, female patients with HFrEF have lower relative mRNA expression for OPA1 (A), PGC‐1α (C), SOD2 (D), NDUFS1 (E), and NDUFS3 (F) ( n = 10 per group for each sex). * P < 0.05 using unpaired Student's t ‐tests. ** P < 0.01 using unpaired Student's t ‐tests. FIS1, mitochondrial fission 1; OPA1, optic atrophy 1; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; NDUFS3, NADH:ubiquinone oxidoreductase core subunit S3; PGC‐1α, peroxisome proliferator‐activated receptor γ coactivator‐1α; SOD2, superoxide dismutase 2.

Article Snippet: Primers were purchased from Qiagen including PGC‐1α, superoxide dismutase 2 (SOD2), FIS1, OPA1, NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), and NADH:ubiquinone oxidoreductase core subunit S3 (NDUFS3).

Techniques: Expressing

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Divergent skeletal muscle mitochondrial phenotype between male and female patients with chronic heart failure

doi: 10.1002/jcsm.12488

Figure Lengend Snippet: Correlations between gene transcript expression levels with peak pulmonary oxygen uptake (V̇O 2peak ) and measures of mitochondrial function and content in heart failure with reduced ejection fraction patients

Article Snippet: Primers were purchased from Qiagen including PGC‐1α, superoxide dismutase 2 (SOD2), FIS1, OPA1, NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), and NADH:ubiquinone oxidoreductase core subunit S3 (NDUFS3).

Techniques: Expressing