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Image Search Results
Journal: Oncogene
Article Title: Disruption of androgen receptor-cofactor interactions by the RNA-binding protein FUS/TLS alters androgen signalling in prostate cancer
doi: 10.1038/s41388-026-03682-3
Figure Lengend Snippet: a COS-1 were transfected with a Gal4 luciferase reporter and β-galactosidase expression plasmid, expression plasmids for Gal4 fused full-length of truncations of FUS and p300, SRC1 or ARA70. b COS-1 were transfected with plasmids encoding the AR, SRC-1, FUS and β-galactosidase, and an AR-responsive luciferase reporter. Luciferase activity was expressed as a % of AR activity in the presence of mibolerone (MIB) and absence of SRC1 and FUS. Mean ± 1SE of 3 independent repeats in duplicate. c Immunoblotting was performed to confirm successful expression of the AR, SRC1 and FUS. d LNCaP FUS were treated ± MIB ± DOX and chromatin immunoprecipitation performed using antibodies specific to the AR, SRC1, RNA Pol II, FUS, or IgG control. Enrichment of the KLK3 enhancer was analysed using qPCR. Mean ± 1SE of 3 independent repeats. ANOVA * p < 0.05, ** p < 0.005, *** p < 0.0005.
Article Snippet: Immunoblotting was performed as previously described [ ] using the following primary antibodies: AR (N-20), FUS (4H11), EWS (G-5) antibodies were from Santa Cruz (CA, USA),
Techniques: Transfection, Luciferase, Expressing, Plasmid Preparation, Activity Assay, Western Blot, Chromatin Immunoprecipitation, Control