Journal: Nature

Article Title: Clearance of senescent glial cells prevents tau -dependent pathology and cognitive decline

doi: 10.1038/s41586-018-0543-y

Figure Lengend Snippet: a–e , Gating strategy ( a ) for FACS isolation of living astrocytes ( b ), microglia ( c ), oligodendrocytes ( d ), and neuron-enriched Cd56 + cells ( e ) from cortices from 6-month-old WT and PS19 mice. b, Astrocyte (Cd11b – ,Cd45 – ,O1 – ,GLAST + ,Cd56 – ) fraction (left) and RT-qPCR analysis (right). c , Microglia (Cd11b + ,Cd45 + ,O1,GLAST + ,Cd56 – ) fraction (left) and RT-qPCR analysis (right). d, Oligodendrocyte (Cd11b – ,Cd45 – ,O1 + ,GLAST – ,Cd56 – ) fraction (left) and RT-qPCR analysis (right). e , Neuron-enriched Cd56 + (Cd11b – ,Cd45 – ,O1 – ,GLAST – ,Cd56 + ) fraction (left) and RT-qPCR analysis (right). p21 is also known as Cdkn1a ; Pai1 is also known as Serpine1 . Individual numbers of independent animal cell population isolations are indicated in the parentheses above p16 Ink4a columns (2 independent experiments). Data are mean ± s.e.m. * P < 0.05; ** P < 0.01 (unpaired two-sided t -tests with Welch’s correction). Exact P values can be found in the accompanying source data file.

Article Snippet: Samples were then incubated with a viability dye, LIVE/DEAD Aqua (Invitrogen, L34966; 1:250) followed by incubation with Cd11b eFluor 450 (eBioscience, 48–0112-80, 1:100), Cd45 APC eFluor 780 (eBioscience, 47–0451-82; 1:200), Glast1 PE (Miltenyi Biotec, 130–095-821; 1:100), O1 AF 700 (R&D Systems, FAB1327N-100UG; 1:100), and Cd56 APC (R&D Systems, FAB7820A; 1:100).

Techniques: Isolation, Quantitative RT-PCR

Journal: Nature Communications

Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer

doi: 10.1038/s41467-025-66800-x

Figure Lengend Snippet: a Schematic of B2M KO generation in murine SCLC primary cell line. Created in BioRender. Meder, L. (2025) https://BioRender.com/4u5k5v3 . b , c Relative MHC-I or PD-L1 expression of WT and MHC-I KO cells analyzed by flow cytometry, determined by mean fluorescence intensity (MFI) normalized to IgG control. Histograms of one representative experiment are shown ( n = 3 biological replicates). d Schematic of experimental setup showing intravenous injection of MHC-I KO and WT cells into immunocompetent C57BL/6/immunodeficient NSG mice, tissue harvest and subsequent IHC and FACS analyses. Created in BioRender. Meder, L. (2025) https://BioRender.com/w6kb2vb . e Representative images of H&E-stained livers and intestinal lymph node tissues from iv. WT and MHC-I KO injected immunocompetent mice. Scale bars, liver 2.5 mm, for lymph nodes 1 mm. Additional FACS based quantification of liver tumor cell infiltration ( n = 4 mice per group). f Representative images of CD45 and CD3 IHC staining in liver tissue from WT and MHC-I KO-injected mice. Quantification was performed using QuPath analysis with 5 regions of interest (ROIs) analyzed per individual ( n = 2 per group). g Representative histograms showing CD107a expression measured by flow cytometry and corresponding quantification (WT n = 4 mice, MHC-I KO n = 5 mice). h Representative images of H&E- and NCAM1-stained liver tissue of MHC-I KO and WT injected immunodeficient NSG mice (MHC-I KO n = 3, WT n = 4) and quantification of tumor cell infiltration in 5 representative ROIs by QuPath analysis. Scale bars, H&E 2.5 mm, NCAM1 100 μm. Statistical analysis was performed using a two-sided, unpaired Student’s t -test. Data in this figure are shown as mean ± SEM. ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.

Article Snippet: The following antibodies and isotype controls were used for staining: CD3 (Alexa-Fluor-700, clone 17A2, Biolegend, Cat. #100216), CD4 (PE-Dazzle 594, clone GK1.5, Biolegend, Cat. # 100456), CD45 (APC-Cy7, clone 30-F11, Biolegend, Cat. # 103116), CTLA-4 (PE, UC10-4B9 Thermo Fisher, Cat. #14-1522-82), CD56 (APC, R&D Systems, clone 809220, Cat. # FAB7820A), CD8a (FITC, clone 53-6.7, Biolegend, Cat. # 100705; Pacific blue, clone 53-6.7, Biolegend, Cat. # 100728), H2Kb (Pacific Blue, clone AF6-88.5, Biolegend, Cat. # 116517), PD-1 (APC, clone 29 F.1A12, Biolegend, Cat. # 135210), PD-L1 (PE-Cy7, clone 10 F.9G2, Biolegend, Cat. # 124313), TIM-3 (PerCP-Cy5.5, clone B8.2C12, Biolegend, Cat. # 134011), Rat IgG2aΚ (Biolegend FITC, Cat. # 400505, PE, Cat. # 400507, PerCP-Cy5.5, Cat. # 400531, APC, Cat. # 400511, Alexa Fluor 700, Cat. # 400528), PE-Dazzle594 Armenian Hamster IgG (PE-Dazzle594, clone HTK888, Biolegend, Cat. # 400951), Rat IgG2bΚ (PE-Cy7, clone RTK4530, Biolegend, Cat. # 400617) and mouse BALB/c IgG2aΚ (Pacific Blue, clone G155-178, BD Biosciences, Cat. # 558118).

Techniques: Expressing, Flow Cytometry, Fluorescence, Control, Injection, Staining, Immunohistochemistry