Journal: Journal of applied microbiology

Article Title: Lactococcus lactis subsp lactis CRL1655 and Schleiferilactobacillus perolens CRL1724 inhibit the adherence of common bovine mastitis pathogens to mammary gland cells, without causing histological changes in the mammary gland.

doi: 10.1111/jam.15604

Figure Lengend Snippet: FIGURE 2 Associated bacteria to MAC-T cells of (a) Schleiferilactobacillus perolens CRL1724, (b) Lactococcus lactis subsp. lactis CRL1655 and (c) the combination of both strains at different MOIs (d). Black bars: S. perolens CRL1724; light grey bars: L. lactis subsp. lactis CRL1655; dark grey bars: L. lactis subsp. lactis CRL1655 + S. perolens CRL1724. Comparison of the associated bacteria between strains. Data are expressed as the Log10 CFU ml−1 mean ± SE. Different letters indicate statistical differences within each MOI evaluated (p < 0.05)

Article Snippet: On the other hand, Bouchard F I G U R E 3 Inhibition of adherence of bovine mastitis pathogens to MAC- T cells pre- treated with Schleiferilactobacillus perolens CRL1724 or Lactococcus lactis subsp. lactis CRL1655. (a) Staphylococcus aureus RC108. (b) Staphylococcus chromogenes. (c) Escherichia coli ATCC 35218. (d) Streptococcus uberis UT102.

Techniques: Bacteria, Comparison

Journal: Journal of applied microbiology

Article Title: Lactococcus lactis subsp lactis CRL1655 and Schleiferilactobacillus perolens CRL1724 inhibit the adherence of common bovine mastitis pathogens to mammary gland cells, without causing histological changes in the mammary gland.

doi: 10.1111/jam.15604

Figure Lengend Snippet: FIGURE 3 Inhibition of adherence of bovine mastitis pathogens to MAC-T cells pre-treated with Schleiferilactobacillus perolens CRL1724 or Lactococcus lactis subsp. lactis CRL1655. (a) Staphylococcus aureus RC108. (b) Staphylococcus chromogenes. (c) Escherichia coli ATCC 35218. (d) Streptococcus uberis UT102. The adhesion assay of each pathogen alone was used as a reference (black bars). Adherence rates were defined as the adherence of each pathogen in the presence of L. lactis subsp. lactis CRL1655 (light grey bars) or S. perolens CRL1724 (dark grey bars), with respect to the adhered pathogen in the reference experiments. Data are expressed as the mean ± SE. Each experiment was carried out by triplicate, and different letters indicate statistical differences in the adherence of pathogens between the treatments (p < 0.05)

Article Snippet: On the other hand, Bouchard F I G U R E 3 Inhibition of adherence of bovine mastitis pathogens to MAC- T cells pre- treated with Schleiferilactobacillus perolens CRL1724 or Lactococcus lactis subsp. lactis CRL1655. (a) Staphylococcus aureus RC108. (b) Staphylococcus chromogenes. (c) Escherichia coli ATCC 35218. (d) Streptococcus uberis UT102.

Techniques: Inhibition, Cell Adhesion Assay

Journal: Journal of Traditional Chinese Medicine

Article Title: Tongqiao Yizhi granule (通窍益智颗粒) repress the nuclear factor kappa-b/nucleotide oligomerization domain-like receptors 3/caspase-1 pyroptosis pathway in the hippocampus to counter vascular dementia in rats

doi: 10.19852/j.cnki.jtcm.20240515.005

Figure Lengend Snippet: A: expression of caspase-1 protein in rat hippocampus (× 200); A1: Sham; A2: Model; A3: NMDP; A4: TQYZKL of 6.2 g·kg-1·d-1; A5: TQYZKL of 12.4 g·kg-1·d-1; A6: TQYZKL of 24.8 g·kg-1·d-1; B: expression of GSDMD protein in rat hippocampus (× 200), B1: Sham; B2: Model; B3: NMDP; B4: TQYZKL of 6.2 g·kg-1·d-1; B5: TQYZKL of 12.4 g·kg-1·d-1; B6: TQYZKL of 24.8 g·kg-1·d-1; Dyeing method of all pictures are the immunohistochemical method; C: expression of IL-18 and IL-1β in serum of rats. Sham and Model group: treated with physiological saline; NMDP group: treated with nimodipine (6.25 mg·kg-1·d-1); 6.2, 12.4, 24.8 group: treated with TQYZKL (6.2 g·kg-1·d-1), TQYZKL (12.4 g·kg-1·d-1), TQYZKL (24.8 g·kg-1·d-1) respectively. TQYZKL: Tongqiao Yizhi granule; NF-κB: nuclear factor kappa-B; NLRP3: nucleotide oligomerization domain-like receptors 3; IHC: immunohistochemistry; ELISA: enzyme-linked immunosorbent assay; IL-18: interleukin-18; IL-1β: interleukin-1β; NMDP: nimodipine. Data are presented as mean ± standard error of the mean (n = 4). Significant differences compared with Sham group were designated as aP < 0.05, with Model group as bP < 0.05 and with NMDP group with cP < 0.05.

Article Snippet: Anti-Golgi peripheral membrane protein p65 (P65) antibody (8242, CST, Boston, USA); anti-NLRP3 antibody (AF2155, Beyotime, Shanghai, China); anti-caspase-1 antibody (22915-1-AP, Proteintech, Chicago, IL, USA); Anti-Gasdermin D (GSDMD) antibody (AF4012, Affinity Biosciences, Cincinnati, OH, USA); anti-β-actin antibody (4967S, CST, Boston, MA, USA); horseradish peroxidase-conjugated anti-rabbit secondary antibody (7074S, CST, Boston, MA, USA); reverse transcription kit (R2028, UE, Beijing, China); real-time fluorescence quantitative polymerase chain reaction (PCR) kit (S2024, UE, Beijing, China); Tunel kit (C1090, Beyotime, Shanghai, China); Hematoxylin-eosin (HE) staining kit (C0105S, Beyotime, Shanghai, China); immunohistochemical staining kit (PV-9001, Zhongshan Golden Bridge, Beijing, China); enzyme-linked immunosorbent assay (ELISA) rat Interleukin-18 (IL-18) ELISA kit (ERC010.96, eBioscience, San Diego, CA, USA); ELISA rat Interleukin-1β (IL-1β) ELISA kit (ERC007.96, eBioscience, San Diego, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Saline, Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC.

doi: 10.1002/advs.202206878

Figure Lengend Snippet: Figure 1. SQLE is a critical mediator of cisplatin resistance in HNSCC. a) Schematic representation of the in vivo model development for cisplatin resistance using HNSCC cell line, SCC-1, in mice bearing HNSCC xenografts. b) Cholesterol content comparison in vehicle, cisplatin-sensitive, and cisplatin-resistant tumors (n = 6). c) Quantitative real-time PCR (qRT-PCR) analysis of cholesterol-related metabolic enzyme expression in cisplatin- sensitive and cisplatin-resistant tumors (n = 6). d) Western blot analysis of SQLE expression in cisplatin-sensitive and cisplatin-resistant tumors (n = 6).

Article Snippet: Cisplatin (#HY-15925), water-soluble cholesterol (#HY-N0322A), terbinafine (#HY-17395A), and NB598 (HY-16343) were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: In Vivo, Comparison, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC.

doi: 10.1002/advs.202206878

Figure Lengend Snippet: Figure 2. SQLE depletion restores cisplatin sensitivity in cisplatin-resistant HNSCC cells both in vitro and in vivo. a,b) Colony formation potential of SCC-1cisR, SCC-23cisR, and CAL-27cisR cells with SQLE depletion, with or without cisplatin treatment (n = 4). Cisplatin concentrations: SCC-1cisR, 8 μg mL−1; SCC-23cisR, 5 μg mL−1; CAL-27cisR, 8 μg mL−1. SQLE knockdown efficiency determined by western blotting. c) Apoptosis rate of SCC-1cisR and SCC-23cisR cells with SQLE depletion in the presence or absence of cisplatin (n = 4). d) Cell viability of SCC-1cisR, SCC-23cisR, and CAL-27cisR cells with SQLE depletion following dose-gradient cisplatin exposure (n = 4). e–g) Effect of SQLE depletion on tumor growth in SCC-1cisR cells with or without

Article Snippet: Cisplatin (#HY-15925), water-soluble cholesterol (#HY-N0322A), terbinafine (#HY-17395A), and NB598 (HY-16343) were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: In Vitro, In Vivo, Knockdown, Western Blot

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC.

doi: 10.1002/advs.202206878

Figure Lengend Snippet: Figure 8. SQLE is crucial for HNSCC initiation and progression, and pharmacological inhibition of SQLE sensitizes cancer cells to cisplatin in vivo. a) Schematic diagram illustrating the determination of Sqle conditional KO effects in oral epithelial cells on HNSCC initiation. b,c) Representative images of tongue lesions and tumor lesion area in SqleCTRL and SqlecKO mice (n = 12). d) Representative histological images of tongue lesions in SqleCTRL and SqlecKO mice. e) Metastatic lymph nodes in SqleCTRL and SqlecKO groups (n = 12). f) Schematic diagram evaluating the effects of Sqle conditional KO on HNSCC development. g,h) Representative gross and histological images of tongue lesions in SqleCTRL and SqlecKO mice. i,j) Tumor lesion area and

Article Snippet: Cisplatin (#HY-15925), water-soluble cholesterol (#HY-N0322A), terbinafine (#HY-17395A), and NB598 (HY-16343) were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Inhibition, In Vivo