Journal: Immunology

Article Title: Lentiviral calnexin-modified dendritic cells promote expansion of high-avidity effector T cells with central memory phenotype.

doi: 10.1111/j.1365-2567.2009.03067.x

Figure Lengend Snippet: Figure 6. Increased CCR7 expression in calnexin–dendritic cell (CNX-DC)-activated T cells. (a) Flow cytometry analysis of memory markers in T cells after coculture with lentiviral vector (LV)-modi- fied DCs. T cells were cocultured with different LV-modified DCs as indicated, restimulated for 7 days and stained with GLC-pentamer together with anti-CD8 antibody and antibodies against CCR7, CD62L, CD28 or CD69. GLC pentamer-positive T cells were CD8- gated and analysed for the expression of differentiation markers. The percentages of different cell population are indicated in the flow graphs. (b) Quantitative analysis of CCR7+ cells in pentamer-positive T cells. Data are representative of four experiments.

Article Snippet: Other fluorochromeconjugated antibodies used in this study included FITC-CCR7 (R&D Systems Inc., Minneapolis, MN), FITC-conjugated human leucocyte antigen DR (HLA-DR) (Caltag Laboratories, Invitrogen, Carlsbad, CA), APC-DCSIGN and APC-CD28 (eBioscience, San Diego, CA).

Techniques: Expressing, Flow Cytometry, Plasmid Preparation, Staining

Journal: Biochemical and biophysical research communications

Article Title: Overexpression of Snail in retinal pigment epithelial triggered epithelial-mesenchymal transition.

doi: 10.1016/j.bbrc.2014.02.119

Figure Lengend Snippet: Fig. 3. Overexpression of Snail in ARPE-19 cells induced EMT. ARPE-19 cells were transfected with pReceiver-Snail or pReceiver-control for 48 h. QRT-PCR and Immunoblotting were used to examine the expression of Snail, E-cadherin, ZO-1, a-SMA and fibronectin. (A) QRT-PCR analysis showed the increased Snail, fibronectin and a- SMA mRNA expression and decreased E-cadherin and ZO-1 mRNA expression. ⁄⁄P < 0.01 vs pReceiver-control. (B) Immunoblotting confirmed the expression of these EMT markers at protein levels.

Article Snippet: The primary antibodies used were as follows: 1:500 E-cadherin antibody and 1:1000 fibronectin antibody (R&D systems, Inc., USA), 1:1000 Snail antibody (Abcam Ltd., Cambridge, USA), 1:1000 a-SMA antibody (Sigma–Aldrich, MO, USA), 1:1000 ZO-1 antibody (Invitrogen, Carlsbad, CA), and 1:5000 GAPDH (Good HERE, Hangzhou, China).

Techniques: Over Expression, Transfection, Control, Quantitative RT-PCR, Western Blot, Expressing

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 3. RSV treatment reduced the levels of inflammatory and angiogenic molecules.Protein levels of VEGF (A), ICAM-1 (B) and MCP-1 (C) were increased 3 days after CNV induction. RSV pretreatment significantly suppressed these increases. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques:

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 5. RSV inhibited NF-κB and inflammatory and angiogenic molecules in vitro. ARPE-19 cells (A–C), b-End3 microvascular endothelial cells (D–F) and RAW264.7 macrophages (G and H) were stimulated with TNF-α. RSV significantly suppressed the phosphorylation (activation) of NF-κB by TNF-α (A, D and G) and the subsequent induction of VEGF (B and H), MCP-1 (C and F) and ICAM-1 (E). All of the effects of RSV were attenuated by an AMPK inhibitor, compound C. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: In Vitro, Phospho-proteomics, Activation Assay

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 7. A model for the preventive effects of RSV on CNV.RSV treatment significantly suppresses CNV development by preserving AMPK activation, which inhibits macrophage recruitment as well as the induction of inflammatory processes, including NF-κB activation and the subsequent up-regulation of inflammatory and angiogenic molecules such as ICAM-1, MCP-1 and VEGF in multiple cell types. PC, laser PC for CNV induction; Vascular EC, vascular endothelial cells.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: Preserving, Activation Assay