Journal: Bio-protocol

Article Title: Mouse Phrenic Nerve Hemidiaphragm Assay (MPN)

doi: 10.21769/BioProtoc.2759

Figure Lengend Snippet: A. Setup overview. 1) Muscle chamber; 2) Stimulator; 3) Tension transducer; 4) Data acquisition system; 5) Computer system with Labscribe software. B. Muscle chamber details. 6) Support system; 7) Static stimulation chamber; 8) Muscle support with oxygen cannula; 9) Micromanipulator; 10) Platinum electrodes; 11) Screw Positioner; 12) Transducer sensor.

Article Snippet: Volumetric flask Clamp (Bulldog Clamp, Diethrich) (Rudolf, catalog number: RU 3934-16; or similar) 2 x scissors (Delicate Surgical Scissors) (Rudolf, catalog number: RU 1503-12) 2 x forceps (Micro Jewelers Forceps, curved) (Rudolf, catalog number: RU 4240-07) Stimulator: 6002 Stimulator (Harvard Apparatus, model: 6002 Basic Stimulator) 2 x micromanipulator (three-dimensional coarse manual manipulator) (NARISHIGE, catalog number: M-152) 2 x Tension transducer: isometric transducer (Harvard Apparatus, catalog number: 72-4494) 2 x Lead Screw Positioner (Harvard Apparatus, catalog number: 53-2082W) 2 x Support system (to hold both stimulation chamber and transducer sensors) 2 x Static stimulation chamber (jacketed, total internal volume 5 ml) Thermostatic bath (Isco, catalog number: GTR190) Recording unit: i-WORX 118 system (iWORX, model: iWORX 118) Computer compatible with the software Gas tank (95% O 2 , 5% CO 2 ) equipped with pressure control (Air Liquide, model: HBS 240-1-2)

Techniques: Software

Journal: STAR Protocols

Article Title: Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system

doi: 10.1016/j.xpro.2023.102623

Figure Lengend Snippet:

Article Snippet: Manipulator (UM-3C) , Narishige Group , Cat# UM-3C.

Techniques: Recombinant, Software, Imaging