Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Cytotoxicity effects after 24 h incubation of increasing concentrations of 7,4′diN(Et) 2 dye with HaCat, SCC-25, and A375 cells. Results are expressed as the mean ± standard error of the mean (SEM). Two-way analysis of variance (two-way ANOVA) was used to determine statistically significant differences between the means of the three cell lines using Sidak’s multiple comparisons tests (**** p value < 0.0001, *** p value < 0.001, ** p value < 0.01, * p value < 0.05).

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Incubation

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: ( A ) Photodynamic effect of 7,4′diN(Et) 2 dye on cellular viability of HaCat, SCC-25, and A374 after a 15-minute irradiation period (white light, 22.5 J/cm 2 ). Results are expressed as the mean ± standard error of the mean (SEM). Two-way analysis of variance (two-way ANOVA) was used to determine statistically significant differences between the three cell lines using Sidak’s multiple comparisons tests (**** p value < 0.0001, *** p value < 0.001, ** p value < 0.01, * p value < 0.05). ( B ) Dose response curves for each cell line, indicating the corresponding IC 50 (half maximal inhibitory concentration) values.

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Irradiation, Concentration Assay

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Photodynamic effect of 7,4′diN(Et) 2 dye at 1.5 µM on cellular viability of HaCat, SCC-25 and A374 after a 15-minute irradiation period (white light, 22.5 J/cm 2 ). Cells were either exposed directly to light in the presence of 7,4′diN(Et) 2 (full colored bars) or washed before irradiation (bars with square pattern). Results are expressed as the mean ± standard error of the mean (SEM). Two-way analysis of variance (two-way ANOVA) was used to determine statistically significant differences between the means of different treatment groups (washed vs. unwashed) within each cell line using Sidak’s multiple comparisons tests (**** p value < 0.0001, *** p value < 0.001, ** p value < 0.01, * p value < 0.05).

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Irradiation

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Uptake efficiency after 30 min of incubation of 7,4′diN(et) 2 with the different cell lines.

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Incubation, Concentration Assay

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Confocal laser scanning microscopy images of HaCat, SCC-25 and A374 after incubation with 3 µM of 7,4′diN(Et) 2 dye. Cells stained with DAPI (blue signal), cellular accumulation of photosensitizer (red signal), and merged images (right panel) showing the localization of the photosensitizer. Scale bar of 20 μm.

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Confocal Laser Scanning Microscopy, Incubation, Staining

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Confocal laser scanning microscopy images of HaCat and A374 CLSM images showing colocalization between lysosomes (marked with LumiTracker ® Lyso Green) and 7,4′diN(Et) 2 after incubation for 30 min. The merged images (right panel) show the lysosomal accumulation of the dye (yellow color). Scale bar of 10 μm.

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Confocal Laser Scanning Microscopy, Incubation

Journal: Scientific Reports

Article Title: Exploring the potential of 7,4′-di(diethylamino)flavylium as a novel photosensitizer for topical photodynamic therapy of skin cancer

doi: 10.1038/s41598-024-80860-x

Figure Lengend Snippet: Confocal laser scanning microscopy images of HaCat, SCC-25 and A374 after being incubated with 3 µM of 7,4′diN(Et) 2 and irradiated (15 min, 22.5 J/cm 2 ). Intracellular ROS generation indicated by the green fluorescence signal of DCDFA. Within each subset of microscopy images, the right panel represents the merge between the DCFA signal and bright field contrast image. Scale bar of 20 μm.

Article Snippet: Human skin keratinocyte cell line (HaCat) was provided by CLS Cell Lines Service GmbH and maintained in DMEM High Glucose Basic (820300a, CLS).

Techniques: Confocal Laser Scanning Microscopy, Incubation, Irradiation, Fluorescence, Microscopy