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Image Search Results
Journal: Molecular cell
Article Title: In vivo evidence for ATPase-dependent DNA translocation by the Bacillus subtilis SMC condensin complex
doi: 10.1016/j.molcel.2018.07.006
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Affinity Purification, Recombinant, Bradford Assay, Activity Assay, Software
Journal: Cell Death Discovery
Article Title: AIM2 regulates autophagy to mitigate oxidative stress in aged mice with acute liver injury
doi: 10.1038/s41420-024-01870-2
Figure Lengend Snippet: A CYP2E1 protein expression and B Immunohistochemical staining of NAPQI-protein adducts were performed, and the images were captured at a 100 µm scale bar. C The mtDNA/nDNA content and D the expression of ND1, TFAM, and CYTB were measured in the liver tissues after 3 h of PBS or APAP supplementation. E Total liver GSH levels were also determined. F Cytoplasmic and mitochondrial proteins from 293 T cells were isolated, and AIM2 was analyzed through western blotting. β-tubulin and VDAC1 served as markers for the cytoplasm (Cy) and mitochondria (Mi), respectively. “Wh” designates the whole lysate. G Co-localization analysis of AIM2 and mitochondria in 293 T cells. AIM2 is highlighted in green, mitochondria in red, DAPI in blue, and co-localized regions are depicted in yellow. Scale bars, 10 µm. H TUNEL staining was conducted 24 h after APAP injection, and the images were captured at a 100 µm scale bar. I The serum IL-6 level and J the relative hepatic mRNA levels were measured in WT and AIM2 −/− mice at 24 h after APAP supplementation. Each group consisted of six mice per time point. Statistical analysis indicated no significance (Ns), * P < 0.05, ** P < 0.01, and *** P < 0.001.
Article Snippet: Antibodies against Cytochrome b (CYTB) (#55090-1-AP), Mitochondrial transcription factor A (TFAM) (#22586-1-AP), VDAC (#55259-1-AP), tubulin (#11224-1-AP), and
Techniques: Expressing, Immunohistochemical staining, Staining, Isolation, Western Blot, TUNEL Assay, Injection