Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: The effects of mitochondrial haplogroups on the expression of mitochondria-related proteins in the livers of the refed hens. ( A ) Representative images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes from immunoblotting analysis. ( B – F ) Quantification of the content of mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 6. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: The effects of mitochondrial haplogroups on the expression of mitochondria-related proteins in the livers of the fasted hens. ( A ) Representative images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes from immunoblotting analysis. ( B – F ) Quantification of the content of mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 6. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. * denotes p < 0.05.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: Impact of mitochondrial haplogroups on mitochondrial protein content in chicken primary hepatocytes upon glucose treatment. ( A ) Representative immunoblotting images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes. ( B – F ) Quantification of the immunoblots for some mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 3. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. ** denotes p < 0.01.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: Impact of mitochondrial haplogroups on mitochondrial protein content in chicken primary hepatocytes upon oleic acid treatment. ( A ) Representative immunoblotting images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes. ( B – F ) Quantification of immunoblots of some mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 3. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. * denotes p < 0.05, and ** denotes p < 0.01.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Western Blot

Journal: Theranostics

Article Title: H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injury

doi: 10.7150/thno.122991

Figure Lengend Snippet: NAD+ emerges as a therapeutic candidate targeting SPHK1-driven kidney injury via activating SIRT1-mediated delactylation. (A) Schematic of high-throughput drug screening workflow in lactate-treated HK-2LPS cells. (B) Venn diagram identified 22 compounds that coordinately downregulate SPHK1 and KIM-1. (C) Top 3 candidates (NAD+, quercetin, and sanguinarine) ranked by efficacy. (D and E) mRNA and protein levels of KIM-1 and SPHK1 in lactate-treated HK-2LPS cells pretreated with sanguinarine/quercetin/NAD+ (n = 5). (F and G) mRNA and protein levels of KIM-1 and SPHK1 in kidney tissue of CLP mice subjected to lactate pretreated with sanguinarine/quercetin/NAD+ (n = 5). (H) Protein levels of pan-Kla in lactate-treated HK-2 cells pretreated with NAD+, NAD+ + EX-527, or resveratrol (n = 5). (I) mRNA levels of SPHK1 and (J) protein levels of SPHK1 and H3K18la in lactate-treated HK-2 cells pretreated with NAD+, NAD+ + EX-527, or resveratrol (n = 5). Data are mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001; ns, not significant.

Article Snippet: In separate experiments, mice were treated with oxamate (MCE, HY-W013032A, 0.5 g/kg), 2-DG (MCE, HY- D13966 , 0.5 g/kg), AC-YVAD-CMK (MCE, HY-16990, 10 mg/kg), Nigericin (MCE, HY-127019, 1 mg/kg), PF-543 (MCE, HY-15425, 10 mg/kg), C646 (MCE, HY-13823, 10 mg/kg), Sanguinarine (MCE, HY-N0052, 10 mg/kg), Quercetin (MCE, HY-18085, 50 mg/kg), NAD + (MCE, HY-B0445, 100 mg/kg), EX-527 (Selleck, S1541, 10 mg/kg), and Resveratrol (Selleck, S1396, 50 mg/kg).

Techniques: High Throughput Screening Assay, Drug discovery

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 1. Characteristics of changes in serum levels of Sirt3 in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 2. Correlation between Sirt3 and clinical indicators in ICU patients. (a) Correlation between serum Sirt3 and SOFA score in sepsis group, septic shock group, and ICU control group. (b) Correlation between serum Sirt3 and lactate in sepsis group, septic shock group, and ICU control group. (c) Correlation between serum Sirt3 and ScvO2 in sepsis group and septic shock group. (d) Correlation between serum Sirt3 and PCT in sepsis group and septic shock group. P < 0.05 were considered statistically significant. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 3. The ROC curves of indicators for the diagnosis of sepsis and the early detection of sepsis from septic shock. (a) The ROC curves of indicators for the diagnosis of sepsis (PCT, Sirt3, SOFA). (b) The ROC curves of indicators for the early detection of septic shock (lactate, Scvo2, Sirt3, lactate + Sirt3, Lactate + ScvO2, P(V-A)CO2). Calculate the predicted values for lactate + Sirt3 and lactate + ScvO2 by binary logistic regression and then calculate the ROC curve. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin, P(V-A)CO2 central venous-to-arterial blood carbon dioxide partial pressure.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Biomarker Discovery

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 4. The ROC curves of predicting for 28-day survival and Kaplan–Meier estimator analysis of Sirt3 for 28 day survival. (a) The ROC curves of Sirt3 for the predicting 28-day mortality in patients with sepsis. P < 0.05 were considered statistically significant (b) Kaplan–Meier estimator analysis of Sirt3 for 28-day survival. The cut off value of Sirt3 for the estimated 28-day mortality of patients with sepsis was 10.07 pg/ml.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: