n370s Search Results


sars-cov-2 (2019-ncov) spike rbd(n370s)-his recombinant protein  (Sino Biological)
N/A
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(N370S)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N370S)) was expressed with a polyhistidine tag at the C-terminus.
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gba n370s/84gg fibroblast cell line  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research gba n370s/84gg fibroblast cell line
Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts <t>(N370S/84GG)</t> derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.
Gba N370s/84gg Fibroblast Cell Line, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n370s/gba+n370s+84gg+fibroblast+cell+line/pmc06467782-451-6-14
Average 90 stars, based on 1 article reviews
gba n370s/84gg fibroblast cell line - by Bioz Stars, 2026-09
90/100 stars
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gba1-n370s mutation  (DWK Life Sciences)
90
DWK Life Sciences gba1-n370s mutation
Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts <t>(N370S/84GG)</t> derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.
Gba1 N370s Mutation, supplied by DWK Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n370s/n370s+mutation/pm37332877-190-9-33
Average 90 stars, based on 1 article reviews
gba1-n370s mutation - by Bioz Stars, 2026-09
90/100 stars
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gba-n370s mutation  (CH Instruments)
90
CH Instruments gba-n370s mutation
Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts <t>(N370S/84GG)</t> derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.
Gba N370s Mutation, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n370s/gba+n370s+mutation/pmc08176903__DAD2___13___e12143___s005-0-4-6
Average 90 stars, based on 1 article reviews
gba-n370s mutation - by Bioz Stars, 2026-09
90/100 stars
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recombinant human glucocerebrosidase  (Genzyme)
86
Genzyme recombinant human glucocerebrosidase
Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts <t>(N370S/84GG)</t> derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.
Recombinant Human Glucocerebrosidase, supplied by Genzyme, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n370s/glucocerebrosidase+n370s+recombinant/us12465586-431-7-10
Average 86 stars, based on 1 article reviews
recombinant human glucocerebrosidase - by Bioz Stars, 2026-09
86/100 stars
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sars cov 2 spike rbd protein n370s mutant mn908947 covid 19 recombinant protein  (OriGene)
N/A
Purified recombinant SARS coronavirus 2 Spike RBD protein Lys310 Leu560 N370S with C terminal His tag secretory expressed in HEK293 cells 50ug
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sars-cov-2 (2019-ncov) spike rbd(n370s)-his recombinant hek293 cell lysate  (Sino Biological)
N/A
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate
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Image Search Results


Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts (N370S/84GG) derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.

Journal: Journal of medicinal chemistry

Article Title: Conversion of Quinazoline Modulators from Inhibitors to Activators of β-Glucocerebrosidase

doi: 10.1021/acs.jmedchem.8b01294

Figure Lengend Snippet: Activator 9q increases GCase protein levels in (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts (N370S/84GG) derived from a GD patient. Cell lysates from fibroblasts treated with vehicle (DMSO), 9q (5 μM, 15 μM), or isofagomine (IFG) were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 4.

Article Snippet: Cell Culture and Compound Treatment The GBA N370S/84GG fibroblast cell line was obtained from Coriell, GM00372,cultured in DMEM medium (Life Tech) including 1% v/v L-glutamine 200 mM (Life Tech), 1% v/v pen strep (Life Tech), 10% FBS (Life Tech) at 37 °C and 5% CO 2 and treated with different compounds at indicated concentrations.

Techniques: Mutagenesis, Derivative Assay, Western Blot

GCase enzyme activity in cell lysates and in the lysosome of 9q-treated cells. GCase activity was measured in lysates from (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts (N370S/84GG). Lysosomal GCase was measured using a live-cell assay in (C) healthy control, (D) homozygous N370S, and (E) homozygous L444P mutant fibroblasts after 3-day treatment with 9q (5 μM, 15 μM), 5 μM isofagomine (IFG), or vehicle (DMSO). The data are presented as the mean ± SEM, n = 3–4; *p<0.05, **p<0,01, ***p<0.001 versus vehicle treatments; one-way ANOVA was followed by the Tukey’s multiple comparisons post hoc test.

Journal: Journal of medicinal chemistry

Article Title: Conversion of Quinazoline Modulators from Inhibitors to Activators of β-Glucocerebrosidase

doi: 10.1021/acs.jmedchem.8b01294

Figure Lengend Snippet: GCase enzyme activity in cell lysates and in the lysosome of 9q-treated cells. GCase activity was measured in lysates from (A) healthy control fibroblasts and (B) compound heterozygous GBA1 mutant fibroblasts (N370S/84GG). Lysosomal GCase was measured using a live-cell assay in (C) healthy control, (D) homozygous N370S, and (E) homozygous L444P mutant fibroblasts after 3-day treatment with 9q (5 μM, 15 μM), 5 μM isofagomine (IFG), or vehicle (DMSO). The data are presented as the mean ± SEM, n = 3–4; *p<0.05, **p<0,01, ***p<0.001 versus vehicle treatments; one-way ANOVA was followed by the Tukey’s multiple comparisons post hoc test.

Article Snippet: Cell Culture and Compound Treatment The GBA N370S/84GG fibroblast cell line was obtained from Coriell, GM00372,cultured in DMEM medium (Life Tech) including 1% v/v L-glutamine 200 mM (Life Tech), 1% v/v pen strep (Life Tech), 10% FBS (Life Tech) at 37 °C and 5% CO 2 and treated with different compounds at indicated concentrations.

Techniques: Activity Assay, Mutagenesis

Activator 9q increases GCase protein levels and enzyme activity in (A) wild-type control and (B) patient-derived heterozygous N370S GBA1 mutant dopaminergic neurons at day 70 of differentiation. Cell lysates from neurons treated with vehicle (DMSO) or 9q (5 μM, 15 μM) for 10 consecutive days were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 3.

Journal: Journal of medicinal chemistry

Article Title: Conversion of Quinazoline Modulators from Inhibitors to Activators of β-Glucocerebrosidase

doi: 10.1021/acs.jmedchem.8b01294

Figure Lengend Snippet: Activator 9q increases GCase protein levels and enzyme activity in (A) wild-type control and (B) patient-derived heterozygous N370S GBA1 mutant dopaminergic neurons at day 70 of differentiation. Cell lysates from neurons treated with vehicle (DMSO) or 9q (5 μM, 15 μM) for 10 consecutive days were analyzed by immunoblotting after no digestion (top), Endo H digestion (middle), and PNGase F digestion (bottom); n = 3.

Article Snippet: Cell Culture and Compound Treatment The GBA N370S/84GG fibroblast cell line was obtained from Coriell, GM00372,cultured in DMEM medium (Life Tech) including 1% v/v L-glutamine 200 mM (Life Tech), 1% v/v pen strep (Life Tech), 10% FBS (Life Tech) at 37 °C and 5% CO 2 and treated with different compounds at indicated concentrations.

Techniques: Activity Assay, Derivative Assay, Mutagenesis, Western Blot

GCase enzyme activity in protein lysates of (A) wild-type control and (B) patient-derived heterozygous N370S GBA1 mutant dopaminergic neurons after 10 days consecutive treatment with vehicle (DMSO) or 9q (day 70 of differentiation). The data are presented as the mean ± SEM, n = 4–7; *p<0.05, **p<0,01 versus vehicle treatments; one-way ANOVA was followed by the Tukey’s multiple comparisons post hoc test.

Journal: Journal of medicinal chemistry

Article Title: Conversion of Quinazoline Modulators from Inhibitors to Activators of β-Glucocerebrosidase

doi: 10.1021/acs.jmedchem.8b01294

Figure Lengend Snippet: GCase enzyme activity in protein lysates of (A) wild-type control and (B) patient-derived heterozygous N370S GBA1 mutant dopaminergic neurons after 10 days consecutive treatment with vehicle (DMSO) or 9q (day 70 of differentiation). The data are presented as the mean ± SEM, n = 4–7; *p<0.05, **p<0,01 versus vehicle treatments; one-way ANOVA was followed by the Tukey’s multiple comparisons post hoc test.

Article Snippet: Cell Culture and Compound Treatment The GBA N370S/84GG fibroblast cell line was obtained from Coriell, GM00372,cultured in DMEM medium (Life Tech) including 1% v/v L-glutamine 200 mM (Life Tech), 1% v/v pen strep (Life Tech), 10% FBS (Life Tech) at 37 °C and 5% CO 2 and treated with different compounds at indicated concentrations.

Techniques: Activity Assay, Derivative Assay, Mutagenesis