Journal: Microorganisms

Article Title: Monosodium Glutamate Inhibits Pseudomonas aeruginosa -Induced Acute Lung Injury by Targeting the Type III Secretion Systems and Modulating Host Immunity

doi: 10.3390/microorganisms14030725

Figure Lengend Snippet: KEGG pathway enrichment analysis and key protein expression in P. aeruginosa -induced acute lung injury (ALI) in mice. ( A ) KEGG pathway enrichment of differentially expressed genes between the ALI model and control groups; ( B ) protein levels of phospho-JNK, p-p38, p-p65, TLR4, and MyD88 in lung tissues compared between the ALI model and control groups by Western blot analysis (β-actin loading control); ( C ) comparison of protein levels (p-JNK, p-p38, p-p65, TLR4, MyD88) in lung tissues between the model and MSG-treated groups, as detected by Western blot (β-actin control) (n = 12 samples per group; data represent means ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The antibodies for phospho-NF-κB p65 (82335-1-RR), p-65 (80979-1-RR), phospho-P38 (14064-1-AP), phospho-JNK (80024-1-RR), MyD88 (23230-1-AP), TLR-4 (19811-1-AP), P38 (14064-1-AP), JNK (51153-1-AP), and β-actin (66009-1-Ig) were purchased from ProteinTech Group (Wuhan, China).

Techniques: Expressing, Control, Western Blot, Comparison

Journal: Biomaterials Research

Article Title: Versatile Nanotherapeutics for Enhancing Sonodynamic Therapy/Chemotherapy of Thyroid Cancer through Remodeling Tumor Microenvironment and Synergistic Reactive Oxygen Species Augment

doi: 10.34133/bmr.0338

Figure Lengend Snippet: FL@M enhances oxidative stress and induces apoptosis. (A) ROS production in 8505C cells after different treatments was detected by DCFH-DA. (B) Quantification of intracellular fluorescence intensity of DCFH-DA shown in (A). (C) Lipid oxidation levels in 8505C cells after different treatments were detected by BODIPY. (D) Quantification of intracellular fluorescence intensity of lipid oxidation level shown in (C). (E) The changes in the MMP of 8505C cells after different treatments were detected by JC-1. (F) Quantification of intracellular fluorescence intensity of MMP shown in (E). (G) Flow cytometry was used to assess ROS production after different treatments detected by DCFH-DA. (H) Flow cytometry was used to assess lipid oxidation levels after different treatments detected by BODIPY. (I) Flow cytometry was used to assess MMP after different treatments detected by JC-1. (J) Apoptosis of 8505C cells was assessed by flow cytometry with Annexin V-PE/7AAD staining. (K) Western blotting analysis of GPX4, MMP2, MMP9, MMP1, and E-Cad protein in 8505C cells with various treatments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The primary antibodies used in this study were as follows: GPX4 (ab125066, Abcam), MMP2 (10373-2-AP, Proteintech), MMP9 (10375-2-AP, Proteintech), MMP1 (10371-2-AP, Proteintech), E-cadherin (20874-1-AP, Proteintech), HIF-1α (20960-1-AP, Proteintech), Galectin-3 (82024-1-RR, Proteintech), Vinculin (A2752, ABclonal), and ACTB (AC026, ABclonal).

Techniques: Fluorescence, Flow Cytometry, Staining, Western Blot