Journal: Journal of thermal biology

Article Title: Effects of heat stress exposure on porcine muscle satellite cells.

doi: 10.1016/j.jtherbio.2023.103569

Figure Lengend Snippet: Fig. 4. Expression of myogenic transcription factors in PMSCs exposed to heat stress. (A) Immunostaining images of transcription factors in PMSCs. Cell nuclei were stained with DAPI (blue), Pax7 (green), and MyoD (red). (B, C) Changes in Pax7, MyoD, and MyoG protein levels in PMSCs by heat stress. (D) Gene expression of Pax7, MyoD, and MyoG in PMSCs by heat treatment. Values are presented as the mean ± SE. a-c Different letters represent statistically significant differences among treatment groups (p < 0.01). Scale bar, 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: These membranes were blocked with TBST buffer containing 5% skim milk for 1.5 h at room temperature, and then incubated with primary antibodies against GAPDH (1:5000, monoclonal, MA5-15738, Invitrogen), Bax (1:1000, monoclonal, sc-7480, Santa Cruz), Bcl-2 (1:1000, monoclonal, sc-7382, Santa Cruz), caspase3 (1:1000, monoclonal, NB100-56708, Novusbio), Pax7 (1:1000, monoclonal, DSHB), MyoD (1:1000, polyclonal, 18943-1-AP, Proteintech), MyoG (1:1000, J.

Techniques: Expressing, Immunostaining, Staining, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

doi: 10.3390/ijms21207545

Figure Lengend Snippet: The influence of TiO 2 , PAA, and SiO 2 NPs on L6 cells differentiation and oxidative stress biomarkers. Fold change in gene expression of myosin I (MyhI), myogenin (MyoG), and nuclear factor (erythroid-derived 2)-like 2 (Nrf2), after treating differentiating rat L6 cells for 10 consecutive days with SiO 2 , TiO 2 , and PAA NPs (50 µg/mL). Results are normalized to Actin beta (ACTB) and shown as a fold increase compared to the non-treated control. Means with standard error are shown for three independent experiments, each performed in three replicates. Statistical significance is displayed as follows: * p ≤ 0.05.

Article Snippet: We used TaqMan Universal PCR Master Mix and the following Gene Expression Assays (all from Applied Biosystems): Rn00582415_m1 (for Nrf2), Rn01751056_m1 (for MyhI), and Rn00567418_m1 (for MyoG).

Techniques: Gene Expression, Derivative Assay, Control