Journal: Acta biochimica et biophysica Sinica

Article Title: Germacrone ameliorates acute lung injury induced by intestinal ischemia-reperfusion by regulating macrophage M1 polarization and mitochondrial defects.

doi: 10.3724/abbs.2024164

Figure Lengend Snippet: Figure 1. Germacrone improved I/R-ALI in mice (A) Photographs showing the appearance of the lungs in each group. (B) HE staining of lung tissues from each group. Scale bar: 50 μm. (C) Masson staining of lung tissues from each group. Scale bar: 1000 μm. (D–F) Immunofluorescence staining of collagen III, vimentin, and α-SMA in lung tissues from each group. Scale bar: 50 μm. Red: collagen III-, vimentin-, and α-SMA-positive cells. Blue: DAPI. Sham: control group; I/RI: mice whose superior mesenteric artery was completely clamped; I/RI+Ger: I/RI group administered with germacrone.

Article Snippet: The following primary antibodies were used: TOM20 (1:250, ab186735; Abcam), 8-oxoG (1:100, ab206461; Abcam), Collagen III (1:100, ab184993; Abcam), α-SMA (1:200, 19245S; Cell Signaling Technology, Danvers, USA), Vimentin (1:100, ab92547; Abcam), ICAM-1 (1:50, ab222736; Abcam), and TGF-α (1:100, AF0262; Affinity).

Techniques: Staining, Immunofluorescence, Control

Journal: Journal of nanobiotechnology

Article Title: Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps.

doi: 10.1186/s12951-024-02533-1

Figure Lengend Snippet: Fig. 6 3D-ABs suppress oxidative stress and cell death, and stimulated angiogenesis in ischaemic flaps. (A) F-CHP staining for damaged collagen detec tion in the skin on POD7. Scale bars, 100 μm. (B) The three groups’ quantified F-CHP intensity (n = 6). (C) CD31 and α-SMA IF staining in the flap on POD7. Scale bars, 50 μm. (D) Quantified CD31/α-SMA-positive blood vessel density among the three groups (n = 6). (E) Dead cells in flap tissue sections on POD7 under the detection of TUNEL staining. Scale bar, 20 μm. (F) TUNEL-positive cell percentage in the dermal layer, quantified for each of the three groups (n = 6). (G) DHE staining of the skin tissues on POD7 among the three groups. Scale bars, 50 μm. (H) Quantified DHE among the three groups (n = 6). SEM error bars are used. Significance (*): p value < 0.05; equal variances ANOVA with LSD post hoc analysis or unequal variances Dunnett’s T3 technique

Article Snippet: The primary antibodies utilized were specific to CD31 (1:200), CD68 (1:200), α-SMA (1:200; Proteintech, 67735-1-Ig), iNOS (1:200), and Arg1 (1:200).

Techniques: Staining, TUNEL Assay